Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004963                                                              18    1135
XP_005251512                                                           18    1135
NP_945148                                                              17    3301
NP_689714                                                              17    3301
NP_945147                                                              17    3301
NP_945146                                                              17    3301
XP_006711254                                                           17    3301
NP_066969                                                              15    9605
NP_055591                                                              15    9605
NP_631940                                                              15    9605
NP_476511                                                              15    9605
NP_001128686                                                           15    9605
NP_001128685                                                           15    9605
NP_476510                                                              15    9605
XP_006719777                                                           15    9605
XP_006719776                                                           15    9605
XP_006719775                                                           15    9605
XP_006719774                                                           15    9605
XP_006719773                                                           15    9605
XP_005254057                                                           15    9605
XP_006719772                                                           15    9605
XP_006719771                                                           15    9605
XP_006719770                                                           15    9605
XP_005254054                                                           15    9605
>NP_004963
          Length = 1132

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  NMSALMSET 9
          NM ALMSET
Sbjct: 83 NMFALMSET 91
>XP_005251512
          Length = 1132

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  NMSALMSET 9
          NM ALMSET
Sbjct: 83 NMFALMSET 91
>NP_945148
          Length = 452

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   NMSALMSE 8
           NMS LMSE
Sbjct: 375 NMSKLMSE 382
>NP_689714
          Length = 492

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   NMSALMSE 8
           NMS LMSE
Sbjct: 384 NMSKLMSE 391
>NP_945147
          Length = 461

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   NMSALMSE 8
           NMS LMSE
Sbjct: 384 NMSKLMSE 391
>NP_945146
          Length = 483

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   NMSALMSE 8
           NMS LMSE
Sbjct: 375 NMSKLMSE 382
>XP_006711254
          Length = 492

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   NMSALMSE 8
           NMS LMSE
Sbjct: 384 NMSKLMSE 391
>NP_066969
          Length = 346

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  NMSALMSE 8
          N+S+L+SE
Sbjct: 58 NLSSLLSE 65
>NP_055591
          Length = 679

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NMSALMSET 9
           N SAL++ET
Sbjct: 303 NHSALVTET 311
>NP_631940
          Length = 471

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NMSALMSET 9
           N SAL++ET
Sbjct: 303 NHSALVTET 311
>NP_476511
          Length = 631

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NMSALMSET 9
           N SAL++ET
Sbjct: 303 NHSALVTET 311
>NP_001128686
          Length = 729

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NMSALMSET 9
           N SAL++ET
Sbjct: 303 NHSALVTET 311
>NP_001128685
          Length = 681

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NMSALMSET 9
           N SAL++ET
Sbjct: 305 NHSALVTET 313
>NP_476510
          Length = 759

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NMSALMSET 9
           N SAL++ET
Sbjct: 303 NHSALVTET 311
>XP_006719777
          Length = 421

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NMSALMSET 9
           N SAL++ET
Sbjct: 303 NHSALVTET 311
>XP_006719776
          Length = 456

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NMSALMSET 9
           N SAL++ET
Sbjct: 303 NHSALVTET 311
>XP_006719775
          Length = 596

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NMSALMSET 9
           N SAL++ET
Sbjct: 303 NHSALVTET 311
>XP_006719774
          Length = 626

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NMSALMSET 9
           N SAL++ET
Sbjct: 303 NHSALVTET 311
>XP_006719773
          Length = 674

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NMSALMSET 9
           N SAL++ET
Sbjct: 303 NHSALVTET 311
>XP_005254057
          Length = 708

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NMSALMSET 9
           N SAL++ET
Sbjct: 302 NHSALVTET 310
>XP_006719772
          Length = 709

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NMSALMSET 9
           N SAL++ET
Sbjct: 303 NHSALVTET 311
>XP_006719771
          Length = 714

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NMSALMSET 9
           N SAL++ET
Sbjct: 303 NHSALVTET 311
>XP_006719770
          Length = 744

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NMSALMSET 9
           N SAL++ET
Sbjct: 303 NHSALVTET 311
>XP_005254054
          Length = 758

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NMSALMSET 9
           N SAL++ET
Sbjct: 302 NHSALVTET 310
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.297    0.100    0.223 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 212,476
Number of extensions: 231
Number of successful extensions: 24
Number of sequences better than 10000.0: 24
Number of HSP's gapped: 24
Number of HSP's successfully gapped: 24
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)

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