Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              17    2528
NP_671714                                                              17    2528
XP_005250160                                                           17    2528
XP_005250159                                                           17    2528
NP_055660                                                              16    7355
NP_078789                                                              16    7355
XP_006715685                                                           16    7355
XP_005265541                                                           16    7355
XP_006713398                                                           16    7355
XP_006713397                                                           16    7355
XP_006713396                                                           16    7355
NP_055877                                                              15    9605
NP_001275656                                                           15    9605
NP_689805                                                              15    9605
NP_001275657                                                           15    9605
NP_004125                                                              15    9605
NP_001164120                                                           15    9605
NP_001164121                                                           15    9605
NP_112483                                                              15    9605
NP_001013725                                                           15    9605
NP_803882                                                              15    9605
NP_006860                                                              15    9605
NP_001271240                                                           15    9605
NP_001271239                                                           15    9605
NP_001271238                                                           15    9605
NP_001271237                                                           15    9605
NP_619636                                                              15    9605
XP_005262363                                                           15    9605
XP_005262362                                                           15    9605
XP_005262361                                                           15    9605
XP_005262360                                                           15    9605
XP_005262358                                                           15    9605
XP_005254450                                                           15    9605
XP_005254449                                                           15    9605
XP_006720129                                                           15    9605
XP_006720128                                                           15    9605
XP_006720126                                                           15    9605
XP_005267433                                                           15    9605
XP_005267434                                                           15    9605
XP_006717794                                                           15    9605
XP_005269715                                                           15    9605
XP_006717793                                                           15    9605
XP_005269713                                                           15    9605
XP_005246758                                                           15    9605
XP_005246757                                                           15    9605
XP_005246756                                                           15    9605
XP_005246755                                                           15    9605
XP_006712707                                                           15    9605
NP_001010893                                                           15    9605
>NP_005742
          Length = 3907

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1    NLVSERE 7
            NLVSERE
Sbjct: 1647 NLVSERE 1653
>NP_671714
          Length = 3899

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1    NLVSERE 7
            NLVSERE
Sbjct: 1647 NLVSERE 1653
>XP_005250160
          Length = 3939

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1    NLVSERE 7
            NLVSERE
Sbjct: 1647 NLVSERE 1653
>XP_005250159
          Length = 3952

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1    NLVSERE 7
            NLVSERE
Sbjct: 1647 NLVSERE 1653
>NP_055660
          Length = 907

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NLVSERE 7
           NLV ERE
Sbjct: 376 NLVKERE 382
>NP_078789
          Length = 1478

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SEREGA 9
           SEREGA
Sbjct: 849 SEREGA 854
>XP_006715685
          Length = 761

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NLVSERE 7
           NLV ERE
Sbjct: 230 NLVKERE 236
>XP_005265541
          Length = 1080

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SEREGA 9
           SEREGA
Sbjct: 849 SEREGA 854
>XP_006713398
          Length = 1478

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SEREGA 9
           SEREGA
Sbjct: 849 SEREGA 854
>XP_006713397
          Length = 1478

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SEREGA 9
           SEREGA
Sbjct: 849 SEREGA 854
>XP_006713396
          Length = 1478

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SEREGA 9
           SEREGA
Sbjct: 849 SEREGA 854
>NP_055877
          Length = 1664

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LVSEREGA 9
           LVS REG+
Sbjct: 155 LVSPREGS 162
>NP_001275656
          Length = 1400

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LVSEREGA 9
           LVS REG+
Sbjct: 155 LVSPREGS 162
>NP_689805
          Length = 975

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NLVSEREGA 9
           NL+ E EG+
Sbjct: 794 NLLQEEEGS 802
>NP_001275657
          Length = 1542

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  LVSEREGA 9
          LVS REG+
Sbjct: 35 LVSPREGS 42
>NP_004125
          Length = 679

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LVSEREGA 9
           + SEREG+
Sbjct: 655 MASEREGS 662
>NP_001164120
          Length = 717

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   NLVSERE 7
           NL+SER+
Sbjct: 124 NLISERK 130
>NP_001164121
          Length = 675

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  NLVSERE 7
          NL+SER+
Sbjct: 82 NLISERK 88
>NP_112483
          Length = 734

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   NLVSERE 7
           NL+SER+
Sbjct: 124 NLISERK 130
>NP_001013725
          Length = 1649

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    LVSEREGA 9
            LVS++EG+
Sbjct: 1453 LVSDKEGS 1460
>NP_803882
          Length = 462

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   NLVSEREG 8
           N++ ER+G
Sbjct: 237 NIIKERKG 244
>NP_006860
          Length = 374

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LVSEREGA 9
           +++EREGA
Sbjct: 152 VLTEREGA 159
>NP_001271240
          Length = 279

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  LVSEREGA 9
          +++EREGA
Sbjct: 57 VLTEREGA 64
>NP_001271239
          Length = 302

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  LVSEREGA 9
          +++EREGA
Sbjct: 80 VLTEREGA 87
>NP_001271238
          Length = 302

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  LVSEREGA 9
          +++EREGA
Sbjct: 80 VLTEREGA 87
>NP_001271237
          Length = 385

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LVSEREGA 9
           +++EREGA
Sbjct: 163 VLTEREGA 170
>NP_619636
          Length = 760

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   VSEREGA 9
           VSE EGA
Sbjct: 127 VSENEGA 133
>XP_005262363
          Length = 489

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   NLVSERE 7
           NL+SER+
Sbjct: 124 NLISERK 130
>XP_005262362
          Length = 490

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   NLVSERE 7
           NL+SER+
Sbjct: 124 NLISERK 130
>XP_005262361
          Length = 536

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   NLVSERE 7
           NL+SER+
Sbjct: 124 NLISERK 130
>XP_005262360
          Length = 557

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   NLVSERE 7
           NL+SER+
Sbjct: 124 NLISERK 130
>XP_005262358
          Length = 733

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   NLVSERE 7
           NL+SER+
Sbjct: 124 NLISERK 130
>XP_005254450
          Length = 1505

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    LVSEREGA 9
            LVS++EG+
Sbjct: 1453 LVSDKEGS 1460
>XP_005254449
          Length = 1626

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    LVSEREGA 9
            LVS++EG+
Sbjct: 1453 LVSDKEGS 1460
>XP_006720129
          Length = 587

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NLVSEREGA 9
           NL+ E EG+
Sbjct: 406 NLLQEEEGS 414
>XP_006720128
          Length = 934

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NLVSEREGA 9
           NL+ E EG+
Sbjct: 794 NLLQEEEGS 802
>XP_006720126
          Length = 975

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NLVSEREGA 9
           NL+ E EG+
Sbjct: 794 NLLQEEEGS 802
>XP_005267433
          Length = 975

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NLVSEREGA 9
           NL+ E EG+
Sbjct: 794 NLLQEEEGS 802
>XP_005267434
          Length = 975

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NLVSEREGA 9
           NL+ E EG+
Sbjct: 794 NLLQEEEGS 802
>XP_006717794
          Length = 1194

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LVSEREGA 9
           LVS REG+
Sbjct: 155 LVSPREGS 162
>XP_005269715
          Length = 1230

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LVSEREGA 9
           LVS REG+
Sbjct: 155 LVSPREGS 162
>XP_006717793
          Length = 1603

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LVSEREGA 9
           LVS REG+
Sbjct: 155 LVSPREGS 162
>XP_005269713
          Length = 1646

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LVSEREGA 9
           LVS REG+
Sbjct: 155 LVSPREGS 162
>XP_005246758
          Length = 234

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1  NLVSEREG 8
          N++ ER+G
Sbjct: 9  NIIKERKG 16
>XP_005246757
          Length = 234

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1  NLVSEREG 8
          N++ ER+G
Sbjct: 9  NIIKERKG 16
>XP_005246756
          Length = 360

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   NLVSEREG 8
           N++ ER+G
Sbjct: 135 NIIKERKG 142
>XP_005246755
          Length = 360

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   NLVSEREG 8
           N++ ER+G
Sbjct: 135 NIIKERKG 142
>XP_006712707
          Length = 444

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   NLVSEREG 8
           N++ ER+G
Sbjct: 219 NIIKERKG 226
>NP_001010893
          Length = 438

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  NLVSEREG 8
          NLV++ EG
Sbjct: 89 NLVTDEEG 96
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.298    0.121    0.297 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 543,120
Number of extensions: 841
Number of successful extensions: 49
Number of sequences better than 10000.0: 49
Number of HSP's gapped: 49
Number of HSP's successfully gapped: 49
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.1 bits)
S2: 28 (15.4 bits)

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