Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_958441                                                              20     390
NP_958439                                                              20     390
NP_958440                                                              20     390
NP_005219                                                              20     390
NP_542391                                                              17    3301
XP_005250749                                                           17    3301
XP_006716239                                                           17    3301
NP_064504                                                              17    4312
NP_078901                                                              17    4312
XP_005250558                                                           17    4312
NP_001973                                                              16    5631
NP_599023                                                              16    7355
NP_599022                                                              16    7355
NP_689969                                                              16    7355
NP_001272368                                                           16    7355
NP_775099                                                              16    7355
NP_056472                                                              16    7355
NP_599024                                                              16    7355
NP_002934                                                              16    7355
NP_071926                                                              16    7355
XP_005254641                                                           16    7355
XP_005268557                                                           16    7355
XP_006714856                                                           16    7355
XP_005268556                                                           16    7355
XP_006714855                                                           16    7355
XP_006714854                                                           16    7355
XP_005268555                                                           16    7355
XP_005268554                                                           16    7355
XP_006714526                                                           16    7355
NP_001036064                                                           15    9605
NP_005226                                                              15    9605
NP_003627                                                              15    9605
NP_001186791                                                           15    9605
NP_001013433                                                           15    9605
NP_742128                                                              15    9605
NP_001186792                                                           15    9605
NP_060785                                                              15    9605
NP_361014                                                              15    9605
NP_001186789                                                           15    9605
NP_001186790                                                           15    9605
NP_001036009                                                           15    9605
NP_001036010                                                           15    9605
NP_003564                                                              15    9605
XP_005260237                                                           15    9605
XP_005246434                                                           15    9605
XP_005246433                                                           15    9605
XP_005246432                                                           15    9605
XP_006712427                                                           15    9605
XP_005263571                                                           15    9605
XP_005263570                                                           15    9605
XP_005263569                                                           15    9605
XP_005263568                                                           15    9605
NP_478126                                                              15    9605
>NP_958441
          Length = 705

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   LTKIICAQ 9
           LTKIICAQ
Sbjct: 210 LTKIICAQ 217
>NP_958439
          Length = 628

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   LTKIICAQ 9
           LTKIICAQ
Sbjct: 210 LTKIICAQ 217
>NP_958440
          Length = 405

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   LTKIICAQ 9
           LTKIICAQ
Sbjct: 210 LTKIICAQ 217
>NP_005219
          Length = 1210

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   LTKIICAQ 9
           LTKIICAQ
Sbjct: 210 LTKIICAQ 217
>NP_542391
          Length = 300

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  LTKIICA 8
          LTK++CA
Sbjct: 10 LTKVLCA 16
>XP_005250749
          Length = 226

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  LTKIICA 8
          LTK++CA
Sbjct: 10 LTKVLCA 16
>XP_006716239
          Length = 276

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  LTKIICA 8
          LTK++CA
Sbjct: 10 LTKVLCA 16
>NP_064504
          Length = 902

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  LTKIICA 8
          +TKI+CA
Sbjct: 10 ITKILCA 16
>NP_078901
          Length = 699

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  LTKIICA 8
          +TKI+CA
Sbjct: 10 ITKILCA 16
>XP_005250558
          Length = 1024

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  LTKIICA 8
          +TKI+CA
Sbjct: 10 ITKILCA 16
>NP_001973
          Length = 1342

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   LTKIICA 8
           LTK ICA
Sbjct: 205 LTKTICA 211
>NP_599023
          Length = 523

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   LTKIIC 7
           LTK+IC
Sbjct: 467 LTKLIC 472
>NP_599022
          Length = 556

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   LTKIIC 7
           LTK+IC
Sbjct: 500 LTKLIC 505
>NP_689969
          Length = 1708

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1  NLTKIIC 7
          N TK++C
Sbjct: 62 NFTKLLC 68
>NP_001272368
          Length = 1732

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1  NLTKIIC 7
          N TK++C
Sbjct: 62 NFTKLLC 68
>NP_775099
          Length = 2595

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   NLTKIIC 7
           NLT+++C
Sbjct: 435 NLTELLC 441
>NP_056472
          Length = 2277

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   NLTKIIC 7
           NLT+++C
Sbjct: 117 NLTELLC 123
>NP_599024
          Length = 468

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   LTKIIC 7
           LTK+IC
Sbjct: 412 LTKLIC 417
>NP_002934
          Length = 548

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   LTKIIC 7
           LTK+IC
Sbjct: 492 LTKLIC 497
>NP_071926
          Length = 1544

 Score = 15.8 bits (29), Expect =  7355
 Identities = 3/9 (33%), Positives = 8/9 (88%)

Query: 1   NLTKIICAQ 9
           N+T+++C +
Sbjct: 632 NMTQLLCVE 640
>XP_005254641
          Length = 477

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   LTKIIC 7
           LTK+IC
Sbjct: 421 LTKLIC 426
>XP_005268557
          Length = 934

 Score = 15.8 bits (29), Expect =  7355
 Identities = 3/9 (33%), Positives = 8/9 (88%)

Query: 1   NLTKIICAQ 9
           N+T+++C +
Sbjct: 632 NMTQLLCVE 640
>XP_006714856
          Length = 1346

 Score = 15.8 bits (29), Expect =  7355
 Identities = 3/9 (33%), Positives = 8/9 (88%)

Query: 1   NLTKIICAQ 9
           N+T+++C +
Sbjct: 434 NMTQLLCVE 442
>XP_005268556
          Length = 1466

 Score = 15.8 bits (29), Expect =  7355
 Identities = 3/9 (33%), Positives = 8/9 (88%)

Query: 1   NLTKIICAQ 9
           N+T+++C +
Sbjct: 554 NMTQLLCVE 562
>XP_006714855
          Length = 1473

 Score = 15.8 bits (29), Expect =  7355
 Identities = 3/9 (33%), Positives = 8/9 (88%)

Query: 1   NLTKIICAQ 9
           N+T+++C +
Sbjct: 632 NMTQLLCVE 640
>XP_006714854
          Length = 1486

 Score = 15.8 bits (29), Expect =  7355
 Identities = 3/9 (33%), Positives = 8/9 (88%)

Query: 1   NLTKIICAQ 9
           N+T+++C +
Sbjct: 574 NMTQLLCVE 582
>XP_005268555
          Length = 1524

 Score = 15.8 bits (29), Expect =  7355
 Identities = 3/9 (33%), Positives = 8/9 (88%)

Query: 1   NLTKIICAQ 9
           N+T+++C +
Sbjct: 632 NMTQLLCVE 640
>XP_005268554
          Length = 1531

 Score = 15.8 bits (29), Expect =  7355
 Identities = 3/9 (33%), Positives = 8/9 (88%)

Query: 1   NLTKIICAQ 9
           N+T+++C +
Sbjct: 632 NMTQLLCVE 640
>XP_006714526
          Length = 1670

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1  NLTKIIC 7
          N TK++C
Sbjct: 62 NFTKLLC 68
>NP_001036064
          Length = 1292

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   LTKIICAQ 9
           LT+ +CA+
Sbjct: 208 LTRTVCAE 215
>NP_005226
          Length = 1308

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   LTKIICAQ 9
           LT+ +CA+
Sbjct: 208 LTRTVCAE 215
>NP_003627
          Length = 367

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   NLTKIICAQ 9
           NLT  IC Q
Sbjct: 206 NLTPPICEQ 214
>NP_001186791
          Length = 415

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   NLTKIICAQ 9
           NLT  IC Q
Sbjct: 254 NLTPPICEQ 262
>NP_001013433
          Length = 589

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 3   TKIICA 8
           TK++CA
Sbjct: 562 TKLVCA 567
>NP_742128
          Length = 353

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   NLTKIICAQ 9
           NLT  IC Q
Sbjct: 192 NLTPPICEQ 200
>NP_001186792
          Length = 300

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   NLTKIICAQ 9
           NLT  IC Q
Sbjct: 139 NLTPPICEQ 147
>NP_060785
          Length = 627

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 3   TKIICA 8
           TK++CA
Sbjct: 600 TKLVCA 605
>NP_361014
          Length = 707

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 3   TKIICA 8
           TK++CA
Sbjct: 680 TKLVCA 685
>NP_001186789
          Length = 367

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   NLTKIICAQ 9
           NLT  IC Q
Sbjct: 206 NLTPPICEQ 214
>NP_001186790
          Length = 367

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   NLTKIICAQ 9
           NLT  IC Q
Sbjct: 206 NLTPPICEQ 214
>NP_001036009
          Length = 1624

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   NLTKIIC 7
           N+TK IC
Sbjct: 425 NITKQIC 431
>NP_001036010
          Length = 1557

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   NLTKIIC 7
           N+TK IC
Sbjct: 358 NITKQIC 364
>NP_003564
          Length = 1587

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   NLTKIIC 7
           N+TK IC
Sbjct: 388 NITKQIC 394
>XP_005260237
          Length = 356

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  KIICAQ 9
          KIICA+
Sbjct: 65 KIICAE 70
>XP_005246434
          Length = 1282

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   LTKIICAQ 9
           LT+ +CA+
Sbjct: 208 LTRTVCAE 215
>XP_005246433
          Length = 1298

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   LTKIICAQ 9
           LT+ +CA+
Sbjct: 208 LTRTVCAE 215
>XP_005246432
          Length = 1307

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   LTKIICAQ 9
           LT+ +CA+
Sbjct: 208 LTRTVCAE 215
>XP_006712427
          Length = 1323

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   LTKIICAQ 9
           LT+ +CA+
Sbjct: 208 LTRTVCAE 215
>XP_005263571
          Length = 353

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   NLTKIICAQ 9
           NLT  IC Q
Sbjct: 192 NLTPPICEQ 200
>XP_005263570
          Length = 395

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   NLTKIICAQ 9
           NLT  IC Q
Sbjct: 206 NLTPPICEQ 214
>XP_005263569
          Length = 395

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   NLTKIICAQ 9
           NLT  IC Q
Sbjct: 206 NLTPPICEQ 214
>XP_005263568
          Length = 395

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   NLTKIICAQ 9
           NLT  IC Q
Sbjct: 206 NLTPPICEQ 214
>NP_478126
          Length = 272

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 3  TKIICA 8
          TK++CA
Sbjct: 60 TKVLCA 65
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.329    0.135    0.404 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 559,106
Number of extensions: 757
Number of successful extensions: 53
Number of sequences better than 10000.0: 53
Number of HSP's gapped: 53
Number of HSP's successfully gapped: 53
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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