Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              19     869
NP_001075109                                                           19     869
XP_006723719                                                           18    1482
NP_001010872                                                           16    7355
XP_006715085                                                           16    7355
XP_006714730                                                           16    7355
NP_002535                                                              16    7355
NP_004658                                                              15    9605
NP_060208                                                              15    9605
NP_001181966                                                           15    9605
NP_001071174                                                           15    9605
XP_005260640                                                           15    9605
XP_005260639                                                           15    9605
XP_006720790                                                           15    9605
XP_005268334                                                           15    9605
XP_005268333                                                           15    9605
XP_006720789                                                           15    9605
XP_006716856                                                           15    9605
XP_005251549                                                           15    9605
XP_006715535                                                           15    9605
NP_001004692                                                           15    9605
NP_003601                                                              15    9605
NP_001015885                                                           15    9605
>NP_008835
          Length = 4128

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   NLSSNKAIS 9
           NLSSN+AIS
Sbjct: 839 NLSSNEAIS 847
>NP_001075109
          Length = 4097

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   NLSSNKAIS 9
           NLSSN+AIS
Sbjct: 839 NLSSNEAIS 847
>XP_006723719
          Length = 311

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   NLSSNKAIS 9
           NLSSNK+I+
Sbjct: 272 NLSSNKSIT 280
>NP_001010872
          Length = 1011

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NLSSNKA 7
           NLS NKA
Sbjct: 788 NLSKNKA 794
>XP_006715085
          Length = 1011

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NLSSNKA 7
           NLS NKA
Sbjct: 788 NLSKNKA 794
>XP_006714730
          Length = 338

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NLSSNKAI 8
           NLS NKA+
Sbjct: 300 NLSLNKAL 307
>NP_002535
          Length = 440

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NLSSNK 6
           NLSSNK
Sbjct: 152 NLSSNK 157
>NP_004658
          Length = 4834

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    NLSSNKAI 8
            NL+SN++I
Sbjct: 3742 NLASNRSI 3749
>NP_060208
          Length = 1406

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NLSSNKAIS 9
           N+S+NK  S
Sbjct: 485 NISANKGFS 493
>NP_001181966
          Length = 904

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LSSNKAI 8
           LSSN+AI
Sbjct: 600 LSSNRAI 606
>NP_001071174
          Length = 904

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LSSNKAI 8
           LSSN+AI
Sbjct: 600 LSSNRAI 606
>XP_005260640
          Length = 406

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   NLSSNKAI 8
           +LSSN+AI
Sbjct: 152 DLSSNQAI 159
>XP_005260639
          Length = 434

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   NLSSNKAI 8
           +LSSN+AI
Sbjct: 180 DLSSNQAI 187
>XP_006720790
          Length = 4748

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    NLSSNKAI 8
            NL+SN++I
Sbjct: 3656 NLASNRSI 3663
>XP_005268334
          Length = 4796

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    NLSSNKAI 8
            NL+SN++I
Sbjct: 3704 NLASNRSI 3711
>XP_005268333
          Length = 4796

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    NLSSNKAI 8
            NL+SN++I
Sbjct: 3704 NLASNRSI 3711
>XP_006720789
          Length = 4829

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    NLSSNKAI 8
            NL+SN++I
Sbjct: 3737 NLASNRSI 3744
>XP_006716856
          Length = 1343

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NLSSNKAIS 9
           N+S+NK  S
Sbjct: 422 NISANKGFS 430
>XP_005251549
          Length = 1405

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NLSSNKAIS 9
           N+S+NK  S
Sbjct: 485 NISANKGFS 493
>XP_006715535
          Length = 946

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LSSNKAI 8
           LSSN+AI
Sbjct: 642 LSSNRAI 648
>NP_001004692
          Length = 320

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  LSSNKAIS 9
          L+ NKAIS
Sbjct: 84 LTGNKAIS 91
>NP_003601
          Length = 368

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   NLSSNKAI 8
           +LSSN+AI
Sbjct: 114 DLSSNQAI 121
>NP_001015885
          Length = 368

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   NLSSNKAI 8
           +LSSN+AI
Sbjct: 114 DLSSNQAI 121
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.287    0.102    0.225 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 341,888
Number of extensions: 538
Number of successful extensions: 23
Number of sequences better than 10000.0: 23
Number of HSP's gapped: 23
Number of HSP's successfully gapped: 23
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.9 bits)
S2: 28 (15.4 bits)

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