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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_444256                                                              19     869
NP_444254                                                              19     869
NP_444253                                                              19     869
NP_444255                                                              19     869
XP_005247551                                                           19     869
XP_005247550                                                           19     869
XP_006713712                                                           19     869
XP_005247549                                                           19     869
NP_065145                                                              17    3301
NP_002606                                                              16    7355
NP_055178                                                              16    7355
NP_004335                                                              16    7355
NP_001264984                                                           16    7355
XP_005266395                                                           16    7355
NP_056991                                                              15    9605
NP_665699                                                              15    9605
NP_066941                                                              15    9605
XP_005262143                                                           15    9605
XP_005258294                                                           15    9605
XP_006722495                                                           15    9605
XP_005258293                                                           15    9605
NP_005870                                                              15    9605

>NP_444256
          Length = 1794

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   NLKSASKE 8
           NLKSASKE
Sbjct: 989 NLKSASKE 996



>NP_444254
          Length = 1845

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   NLKSASKE 8
           NLKSASKE
Sbjct: 989 NLKSASKE 996



>NP_444253
          Length = 1914

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    NLKSASKE 8
            NLKSASKE
Sbjct: 1058 NLKSASKE 1065



>NP_444255
          Length = 1863

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    NLKSASKE 8
            NLKSASKE
Sbjct: 1058 NLKSASKE 1065



>XP_005247551
          Length = 1855

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    NLKSASKE 8
            NLKSASKE
Sbjct: 999  NLKSASKE 1006



>XP_005247550
          Length = 1856

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    NLKSASKE 8
            NLKSASKE
Sbjct: 1068 NLKSASKE 1075



>XP_006713712
          Length = 1923

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    NLKSASKE 8
            NLKSASKE
Sbjct: 1068 NLKSASKE 1075



>XP_005247549
          Length = 1924

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    NLKSASKE 8
            NLKSASKE
Sbjct: 1068 NLKSASKE 1075



>NP_065145
          Length = 199

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  NLKSASKEL 9
          NLK A+KEL
Sbjct: 10 NLKFAAKEL 18



>NP_002606
          Length = 418

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   NLKSASK 7
           NLKSAS+
Sbjct: 135 NLKSASR 141



>NP_055178
          Length = 4579

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NLKSASKEL 9
           NLK  SKE+
Sbjct: 232 NLKDDSKEI 240



>NP_004335
          Length = 172

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NLKSASKEL 9
           NLK  +KEL
Sbjct: 125 NLKRVAKEL 133



>NP_001264984
          Length = 4432

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  NLKSASKEL 9
          NLK  SKE+
Sbjct: 85 NLKDDSKEI 93



>XP_005266395
          Length = 4588

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NLKSASKEL 9
           NLK  SKE+
Sbjct: 232 NLKDDSKEI 240



>NP_056991
          Length = 519

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NLKSASKE 8
           NLKSAS +
Sbjct: 222 NLKSASSK 229



>NP_665699
          Length = 333

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NLKSASKE 8
           N+KSAS++
Sbjct: 195 NVKSASRQ 202



>NP_066941
          Length = 651

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NLKSASK 7
           NLK ASK
Sbjct: 386 NLKKASK 392



>XP_005262143
          Length = 650

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NLKSASK 7
           NLK ASK
Sbjct: 385 NLKKASK 391



>XP_005258294
          Length = 335

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NLKSASKE 8
           N+KSAS++
Sbjct: 195 NVKSASRQ 202



>XP_006722495
          Length = 335

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NLKSASKE 8
           N+KSAS++
Sbjct: 195 NVKSASRQ 202



>XP_005258293
          Length = 335

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NLKSASKE 8
           N+KSAS++
Sbjct: 195 NVKSASRQ 202



>NP_005870
          Length = 469

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LKSASKEL 9
           LKSA K+L
Sbjct: 250 LKSAQKDL 257



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.287    0.106    0.232 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 334,255
Number of extensions: 530
Number of successful extensions: 22
Number of sequences better than 10000.0: 22
Number of HSP's gapped: 22
Number of HSP's successfully gapped: 22
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.8 bits)
S2: 28 (15.4 bits)