Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000258                                                              19     665
NP_001035957                                                           19     665
XP_006721990                                                           19     665
XP_006721989                                                           19     665
XP_005258041                                                           19     665
XP_006721988                                                           19     665
XP_006721987                                                           19     665
XP_006721986                                                           19     665
XP_005258040                                                           19     665
XP_006721985                                                           19     665
NP_006260                                                              17    4312
NP_002653                                                              16    5631
NP_473362                                                              16    5631
NP_000484                                                              16    5631
XP_006715396                                                           16    5631
XP_006715395                                                           16    5631
XP_005247590                                                           16    5631
NP_604391                                                              16    7355
NP_004370                                                              16    7355
NP_001185708                                                           16    7355
NP_003582                                                              16    7355
NP_006381                                                              16    7355
NP_001005739                                                           16    7355
NP_060687                                                              16    7355
NP_057600                                                              16    7355
NP_001185707                                                           16    7355
NP_001185706                                                           16    7355
XP_005257785                                                           16    7355
XP_006712092                                                           16    7355
NP_001138497                                                           16    7355
NP_115510                                                              16    7355
NP_000350                                                              15    9605
NP_001743                                                              15    9605
NP_071328                                                              15    9605
NP_009146                                                              15    9605
NP_003322                                                              15    9605
NP_055798                                                              15    9605
NP_055421                                                              15    9605
NP_115545                                                              15    9605
NP_001258531                                                           15    9605
NP_002504                                                              15    9605
NP_919288                                                              15    9605
NP_065856                                                              15    9605
XP_006722575                                                           15    9605
XP_006721714                                                           15    9605
XP_006714830                                                           15    9605
XP_006714829                                                           15    9605
XP_006714828                                                           15    9605
XP_005263389                                                           15    9605
XP_005263388                                                           15    9605
XP_005263387                                                           15    9605
XP_006714468                                                           15    9605
XP_005263386                                                           15    9605
XP_005263385                                                           15    9605
XP_005263384                                                           15    9605
XP_006712548                                                           15    9605
XP_005246130                                                           15    9605
>NP_000258
          Length = 2818

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NLIHGIVQS 9
            N IHGIVQS
Sbjct: 2658 NPIHGIVQS 2666
>NP_001035957
          Length = 2839

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NLIHGIVQS 9
            N IHGIVQS
Sbjct: 2679 NPIHGIVQS 2687
>XP_006721990
          Length = 2823

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NLIHGIVQS 9
            N IHGIVQS
Sbjct: 2689 NPIHGIVQS 2697
>XP_006721989
          Length = 2823

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NLIHGIVQS 9
            N IHGIVQS
Sbjct: 2689 NPIHGIVQS 2697
>XP_005258041
          Length = 2836

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NLIHGIVQS 9
            N IHGIVQS
Sbjct: 2658 NPIHGIVQS 2666
>XP_006721988
          Length = 2846

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NLIHGIVQS 9
            N IHGIVQS
Sbjct: 2668 NPIHGIVQS 2676
>XP_006721987
          Length = 2849

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NLIHGIVQS 9
            N IHGIVQS
Sbjct: 2689 NPIHGIVQS 2697
>XP_006721986
          Length = 2854

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NLIHGIVQS 9
            N IHGIVQS
Sbjct: 2676 NPIHGIVQS 2684
>XP_005258040
          Length = 2857

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NLIHGIVQS 9
            N IHGIVQS
Sbjct: 2679 NPIHGIVQS 2687
>XP_006721985
          Length = 2867

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NLIHGIVQS 9
            N IHGIVQS
Sbjct: 2689 NPIHGIVQS 2697
>NP_006260
          Length = 2156

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    NLIHGIVQ 8
            N+IHG +Q
Sbjct: 1895 NMIHGTLQ 1902
>NP_002653
          Length = 1074

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NLIHGI 6
           NLIHG+
Sbjct: 595 NLIHGL 600
>NP_473362
          Length = 508

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  NLIHGIVQS 9
          +L HGI++S
Sbjct: 27 SLAHGIIRS 35
>NP_000484
          Length = 680

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1  NLIHGI 6
          NL+HG+
Sbjct: 14 NLVHGV 19
>XP_006715396
          Length = 680

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1  NLIHGI 6
          NL+HG+
Sbjct: 14 NLVHGV 19
>XP_006715395
          Length = 680

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1  NLIHGI 6
          NL+HG+
Sbjct: 14 NLVHGV 19
>XP_005247590
          Length = 1074

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NLIHGI 6
           NLIHG+
Sbjct: 595 NLIHGL 600
>NP_604391
          Length = 341

 Score = 15.8 bits (29), Expect =  7355
 Identities = 3/7 (42%), Positives = 7/7 (100%)

Query: 3  IHGIVQS 9
          +HG++Q+
Sbjct: 66 VHGVIQA 72
>NP_004370
          Length = 327

 Score = 15.8 bits (29), Expect =  7355
 Identities = 3/7 (42%), Positives = 7/7 (100%)

Query: 3  IHGIVQS 9
          +HG++Q+
Sbjct: 66 VHGVIQA 72
>NP_001185708
          Length = 758

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   LIHGIVQS 9
           +IHG++ S
Sbjct: 193 VIHGVINS 200
>NP_003582
          Length = 745

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   LIHGIVQS 9
           +IHG++ S
Sbjct: 180 VIHGVINS 187
>NP_006381
          Length = 1037

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   NLIHGIVQS 9
           N++ GI++S
Sbjct: 95  NIVEGIIRS 103
>NP_001005739
          Length = 965

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NLIHGIVQS 9
           N+IH +V S
Sbjct: 458 NIIHSVVLS 466
>NP_060687
          Length = 578

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   NLIHGI 6
           NL+HG+
Sbjct: 177 NLLHGV 182
>NP_057600
          Length = 977

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NLIHGIVQS 9
           N+IH +V S
Sbjct: 470 NIIHSVVLS 478
>NP_001185707
          Length = 764

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   LIHGIVQS 9
           +IHG++ S
Sbjct: 199 VIHGVINS 206
>NP_001185706
          Length = 745

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   LIHGIVQS 9
           +IHG++ S
Sbjct: 180 VIHGVINS 187
>XP_005257785
          Length = 1887

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   HGIVQS 9
           HG+VQS
Sbjct: 708 HGLVQS 713
>XP_006712092
          Length = 941

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NLIHGIVQS 9
           N+IH +V S
Sbjct: 434 NIIHSVVLS 442
>NP_001138497
          Length = 541

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   NLIHGI 6
           NL+HG+
Sbjct: 140 NLLHGV 145
>NP_115510
          Length = 1663

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   HGIVQS 9
           HG+VQS
Sbjct: 542 HGLVQS 547
>NP_000350
          Length = 817

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/6 (50%), Positives = 6/6 (100%)

Query: 3   IHGIVQ 8
           +HG++Q
Sbjct: 778 VHGVIQ 783
>NP_001743
          Length = 527

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   NLIHGIVQS 9
           N IH  VQS
Sbjct: 507 NAIHTFVQS 515
>NP_071328
          Length = 904

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   NLIHG 5
           NLIHG
Sbjct: 431 NLIHG 435
>NP_009146
          Length = 485

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   NLIHGIVQ 8
           NL+HG ++
Sbjct: 184 NLLHGALE 191
>NP_003322
          Length = 1187

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   NLIHGIV 7
           NL+HG V
Sbjct: 729 NLVHGNV 735
>NP_055798
          Length = 1292

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    IHGIVQS 9
            IH IVQS
Sbjct: 1248 IHDIVQS 1254
>NP_055421
          Length = 1050

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NLIHGIVQ 8
           N I+G+VQ
Sbjct: 421 NTINGVVQ 428
>NP_115545
          Length = 780

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   NLIHG 5
           NLIHG
Sbjct: 217 NLIHG 221
>NP_001258531
          Length = 932

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NLIHGIVQ 8
           N I+G+VQ
Sbjct: 303 NTINGVVQ 310
>NP_002504
          Length = 169

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   NLIHG 5
           NLIHG
Sbjct: 132 NLIHG 136
>NP_919288
          Length = 1005

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 3   IHGIVQ 8
           +HG+VQ
Sbjct: 610 VHGLVQ 615
>NP_065856
          Length = 628

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1  NLIHGI 6
          NL+HG+
Sbjct: 18 NLLHGL 23
>XP_006722575
          Length = 672

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1  NLIHGI 6
          NL+HG+
Sbjct: 62 NLLHGL 67
>XP_006721714
          Length = 330

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   NLIHGIVQ 8
           NL+HG ++
Sbjct: 184 NLLHGALE 191
>XP_006714830
          Length = 1007

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   IHGIVQS 9
           IH IVQS
Sbjct: 963 IHDIVQS 969
>XP_006714829
          Length = 1093

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    IHGIVQS 9
            IH IVQS
Sbjct: 1049 IHDIVQS 1055
>XP_006714828
          Length = 1126

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    IHGIVQS 9
            IH IVQS
Sbjct: 1082 IHDIVQS 1088
>XP_005263389
          Length = 896

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NLIHGIVQ 8
           N I+G+VQ
Sbjct: 421 NTINGVVQ 428
>XP_005263388
          Length = 903

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NLIHGIVQ 8
           N I+G+VQ
Sbjct: 421 NTINGVVQ 428
>XP_005263387
          Length = 993

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NLIHGIVQ 8
           N I+G+VQ
Sbjct: 421 NTINGVVQ 428
>XP_006714468
          Length = 996

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NLIHGIVQ 8
           N I+G+VQ
Sbjct: 367 NTINGVVQ 374
>XP_005263386
          Length = 1042

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NLIHGIVQ 8
           N I+G+VQ
Sbjct: 421 NTINGVVQ 428
>XP_005263385
          Length = 1050

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NLIHGIVQ 8
           N I+G+VQ
Sbjct: 421 NTINGVVQ 428
>XP_005263384
          Length = 1050

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NLIHGIVQ 8
           N I+G+VQ
Sbjct: 421 NTINGVVQ 428
>XP_006712548
          Length = 637

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 3   IHGIVQ 8
           +HG+VQ
Sbjct: 242 VHGLVQ 247
>XP_005246130
          Length = 637

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 3   IHGIVQ 8
           +HG+VQ
Sbjct: 242 VHGLVQ 247
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.323    0.141    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 499,367
Number of extensions: 736
Number of successful extensions: 57
Number of sequences better than 10000.0: 57
Number of HSP's gapped: 57
Number of HSP's successfully gapped: 57
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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