Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_057502                                                              21     229
NP_861969                                                              21     229
NP_000259                                                              21     229
NP_861968                                                              21     229
NP_861966                                                              21     229
NP_861970                                                              21     229
NP_861546                                                              21     229
NP_861967                                                              21     229
NP_588613                                                              17    2528
NP_001008738                                                           17    2528
NP_001035026                                                           17    3301
NP_036273                                                              17    3301
XP_005266398                                                           17    3301
XP_005266397                                                           17    3301
XP_006717717                                                           17    4312
XP_006717716                                                           17    4312
XP_006717715                                                           17    4312
XP_006717714                                                           17    4312
XP_006717713                                                           17    4312
XP_006717712                                                           17    4312
XP_006717711                                                           17    4312
XP_006717710                                                           17    4312
XP_006717709                                                           17    4312
XP_006717708                                                           17    4312
NP_001185924                                                           16    7355
NP_001269695                                                           16    7355
NP_071935                                                              16    7355
NP_056416                                                              16    7355
NP_001265115                                                           16    7355
NP_001265114                                                           16    7355
XP_006717816                                                           16    7355
XP_006717815                                                           16    7355
XP_006717814                                                           16    7355
XP_005251604                                                           16    7355
NP_071362                                                              16    7355
NP_001005174                                                           16    7355
NP_115859                                                              15    9605
NP_061978                                                              15    9605
NP_000843                                                              15    9605
NP_055519                                                              15    9605
NP_000203                                                              15    9605
NP_115582                                                              15    9605
NP_055421                                                              15    9605
NP_001258531                                                           15    9605
NP_060787                                                              15    9605
NP_060733                                                              15    9605
NP_001136336                                                           15    9605
XP_006721417                                                           15    9605
XP_006721416                                                           15    9605
XP_006721363                                                           15    9605
XP_005256258                                                           15    9605
XP_005256257                                                           15    9605
XP_005256256                                                           15    9605
XP_006721362                                                           15    9605
XP_006721361                                                           15    9605
XP_005256254                                                           15    9605
XP_005256253                                                           15    9605
XP_005256252                                                           15    9605
XP_005256251                                                           15    9605
XP_006721360                                                           15    9605
XP_005256250                                                           15    9605
XP_005263389                                                           15    9605
XP_005263388                                                           15    9605
XP_006714468                                                           15    9605
XP_005263386                                                           15    9605
XP_005263385                                                           15    9605
XP_005263384                                                           15    9605
XP_005248229                                                           15    9605
XP_006714030                                                           15    9605
XP_006712103                                                           15    9605
XP_006712102                                                           15    9605
XP_005244872                                                           15    9605
>NP_057502
          Length = 590

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   NLIGDSMSF 9
           NLIGDS+SF
Sbjct: 499 NLIGDSLSF 507
>NP_861969
          Length = 507

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   NLIGDSMSF 9
           NLIGDS+SF
Sbjct: 416 NLIGDSLSF 424
>NP_000259
          Length = 595

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   NLIGDSMSF 9
           NLIGDS+SF
Sbjct: 499 NLIGDSLSF 507
>NP_861968
          Length = 507

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   NLIGDSMSF 9
           NLIGDS+SF
Sbjct: 416 NLIGDSLSF 424
>NP_861966
          Length = 548

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   NLIGDSMSF 9
           NLIGDS+SF
Sbjct: 457 NLIGDSLSF 465
>NP_861970
          Length = 590

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   NLIGDSMSF 9
           NLIGDS+SF
Sbjct: 499 NLIGDSLSF 507
>NP_861546
          Length = 590

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   NLIGDSMSF 9
           NLIGDS+SF
Sbjct: 499 NLIGDSLSF 507
>NP_861967
          Length = 549

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   NLIGDSMSF 9
           NLIGDS+SF
Sbjct: 458 NLIGDSLSF 466
>NP_588613
          Length = 1166

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   LIGDSMS 8
           LIGDSMS
Sbjct: 754 LIGDSMS 760
>NP_001008738
          Length = 1138

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   LIGDSMS 8
           LIGDSMS
Sbjct: 726 LIGDSMS 732
>NP_001035026
          Length = 874

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NLIGDSMSF 9
           NLI DSM +
Sbjct: 425 NLIADSMEY 433
>NP_036273
          Length = 887

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NLIGDSMSF 9
           NLI DSM +
Sbjct: 438 NLIADSMEY 446
>XP_005266398
          Length = 455

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  NLIGDSMSF 9
          NLI DSM +
Sbjct: 6  NLIADSMEY 14
>XP_005266397
          Length = 709

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NLIGDSMSF 9
           NLI DSM +
Sbjct: 260 NLIADSMEY 268
>XP_006717717
          Length = 859

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   GDSMSF 9
           GDSMSF
Sbjct: 203 GDSMSF 208
>XP_006717716
          Length = 1619

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   GDSMSF 9
           GDSMSF
Sbjct: 203 GDSMSF 208
>XP_006717715
          Length = 1660

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   GDSMSF 9
           GDSMSF
Sbjct: 203 GDSMSF 208
>XP_006717714
          Length = 1665

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   GDSMSF 9
           GDSMSF
Sbjct: 203 GDSMSF 208
>XP_006717713
          Length = 1666

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   GDSMSF 9
           GDSMSF
Sbjct: 203 GDSMSF 208
>XP_006717712
          Length = 1670

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   GDSMSF 9
           GDSMSF
Sbjct: 203 GDSMSF 208
>XP_006717711
          Length = 1677

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   GDSMSF 9
           GDSMSF
Sbjct: 203 GDSMSF 208
>XP_006717710
          Length = 1706

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   GDSMSF 9
           GDSMSF
Sbjct: 203 GDSMSF 208
>XP_006717709
          Length = 1712

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   GDSMSF 9
           GDSMSF
Sbjct: 203 GDSMSF 208
>XP_006717708
          Length = 1717

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   GDSMSF 9
           GDSMSF
Sbjct: 203 GDSMSF 208
>NP_001185924
          Length = 414

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  LIGDSMS 8
          LIGD MS
Sbjct: 39 LIGDGMS 45
>NP_001269695
          Length = 349

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NLIGDSM 7
           NLI DSM
Sbjct: 256 NLISDSM 262
>NP_071935
          Length = 419

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NLIGDSM 7
           NLI DSM
Sbjct: 326 NLISDSM 332
>NP_056416
          Length = 1049

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   NLIGDSM 7
           N++GD+M
Sbjct: 709 NIVGDAM 715
>NP_001265115
          Length = 794

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   NLIGDSM 7
           N++GD+M
Sbjct: 454 NIVGDAM 460
>NP_001265114
          Length = 979

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   NLIGDSM 7
           N++GD+M
Sbjct: 717 NIVGDAM 723
>XP_006717816
          Length = 853

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   NLIGDSM 7
           N++GD+M
Sbjct: 717 NIVGDAM 723
>XP_006717815
          Length = 936

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   NLIGDSM 7
           N++GD+M
Sbjct: 596 NIVGDAM 602
>XP_006717814
          Length = 1057

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   NLIGDSM 7
           N++GD+M
Sbjct: 717 NIVGDAM 723
>XP_005251604
          Length = 481

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NLIGDSM 7
           NLI DSM
Sbjct: 388 NLISDSM 394
>NP_071362
          Length = 1057

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   NLIGDSM 7
           N++GD+M
Sbjct: 717 NIVGDAM 723
>NP_001005174
          Length = 321

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LIGDSMSF 9
           L+GD +SF
Sbjct: 310 LLGDKVSF 317
>NP_115859
          Length = 673

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   GDSMSF 9
           GDSMS+
Sbjct: 586 GDSMSY 591
>NP_061978
          Length = 4242

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    GDSMSF 9
            GDSMS+
Sbjct: 4155 GDSMSY 4160
>NP_000843
          Length = 210

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   LIGDSMSF 9
           ++GD +SF
Sbjct: 144 IVGDQISF 151
>NP_055519
          Length = 925

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   NLIGDSMSF 9
           N+IGD   F
Sbjct: 149 NIIGDPADF 157
>NP_000203
          Length = 788

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   IGDSMSF 9
           IGD++SF
Sbjct: 417 IGDTVSF 423
>NP_115582
          Length = 1866

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    NLIGDS 6
            NL+GDS
Sbjct: 1253 NLLGDS 1258
>NP_055421
          Length = 1050

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   NLIGDSM 7
           NL+GD++
Sbjct: 710 NLVGDAL 716
>NP_001258531
          Length = 932

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   NLIGDSM 7
           NL+GD++
Sbjct: 592 NLVGDAL 598
>NP_060787
          Length = 526

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   GDSMSF 9
           GDS+SF
Sbjct: 173 GDSLSF 178
>NP_060733
          Length = 1435

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3    IGDSMSF 9
            +GD MSF
Sbjct: 1354 VGDVMSF 1360
>NP_001136336
          Length = 2521

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    NLIGDSMSF 9
            N+IG  M+F
Sbjct: 1005 NVIGQRMNF 1013
>XP_006721417
          Length = 2497

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    NLIGDSMSF 9
            N+IG  M+F
Sbjct: 1005 NVIGQRMNF 1013
>XP_006721416
          Length = 2504

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NLIGDSMSF 9
           N+IG  M+F
Sbjct: 988 NVIGQRMNF 996
>XP_006721363
          Length = 1385

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    NLIGDS 6
            NL+GDS
Sbjct: 1253 NLLGDS 1258
>XP_005256258
          Length = 1397

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    NLIGDS 6
            NL+GDS
Sbjct: 1222 NLLGDS 1227
>XP_005256257
          Length = 1405

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    NLIGDS 6
            NL+GDS
Sbjct: 1253 NLLGDS 1258
>XP_005256256
          Length = 1427

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    NLIGDS 6
            NL+GDS
Sbjct: 1252 NLLGDS 1257
>XP_006721362
          Length = 1810

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    NLIGDS 6
            NL+GDS
Sbjct: 1253 NLLGDS 1258
>XP_006721361
          Length = 1836

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    NLIGDS 6
            NL+GDS
Sbjct: 1223 NLLGDS 1228
>XP_005256254
          Length = 1837

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    NLIGDS 6
            NL+GDS
Sbjct: 1224 NLLGDS 1229
>XP_005256253
          Length = 1838

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    NLIGDS 6
            NL+GDS
Sbjct: 1225 NLLGDS 1230
>XP_005256252
          Length = 1865

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    NLIGDS 6
            NL+GDS
Sbjct: 1252 NLLGDS 1257
>XP_005256251
          Length = 1866

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    NLIGDS 6
            NL+GDS
Sbjct: 1253 NLLGDS 1258
>XP_006721360
          Length = 1866

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    NLIGDS 6
            NL+GDS
Sbjct: 1253 NLLGDS 1258
>XP_005256250
          Length = 1866

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    NLIGDS 6
            NL+GDS
Sbjct: 1253 NLLGDS 1258
>XP_005263389
          Length = 896

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   NLIGDSM 7
           NL+GD++
Sbjct: 710 NLVGDAL 716
>XP_005263388
          Length = 903

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   NLIGDSM 7
           NL+GD++
Sbjct: 710 NLVGDAL 716
>XP_006714468
          Length = 996

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   NLIGDSM 7
           NL+GD++
Sbjct: 656 NLVGDAL 662
>XP_005263386
          Length = 1042

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   NLIGDSM 7
           NL+GD++
Sbjct: 702 NLVGDAL 708
>XP_005263385
          Length = 1050

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   NLIGDSM 7
           NL+GD++
Sbjct: 710 NLVGDAL 716
>XP_005263384
          Length = 1050

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   NLIGDSM 7
           NL+GD++
Sbjct: 710 NLVGDAL 716
>XP_005248229
          Length = 461

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   GDSMSF 9
           GDS+SF
Sbjct: 108 GDSLSF 113
>XP_006714030
          Length = 495

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   GDSMSF 9
           GDS+SF
Sbjct: 142 GDSLSF 147
>XP_006712103
          Length = 1175

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3    IGDSMSF 9
            +GD MSF
Sbjct: 1094 VGDVMSF 1100
>XP_006712102
          Length = 1313

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3    IGDSMSF 9
            +GD MSF
Sbjct: 1232 VGDVMSF 1238
>XP_005244872
          Length = 961

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   NLIGDSMSF 9
           N+IGD   F
Sbjct: 185 NIIGDPADF 193
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.322    0.138    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 459,972
Number of extensions: 683
Number of successful extensions: 72
Number of sequences better than 10000.0: 72
Number of HSP's gapped: 72
Number of HSP's successfully gapped: 72
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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