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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001121619                                                           19     869
NP_000258                                                              19     869
NP_001035957                                                           19     869
XP_006721991                                                           19     869
XP_006721990                                                           19     869
XP_006721989                                                           19     869
XP_005258041                                                           19     869
XP_006721988                                                           19     869
XP_006721987                                                           19     869
XP_006721986                                                           19     869
XP_005258040                                                           19     869
XP_006721985                                                           19     869
NP_689691                                                              18    1935
XP_005258616                                                           18    1935
XP_005258615                                                           18    1935
NP_001153332                                                           18    1935
NP_001153333                                                           18    1935
NP_065810                                                              17    3301
XP_006720684                                                           17    3301
NP_004517                                                              16    5631
NP_077720                                                              16    7355
NP_037438                                                              16    7355
NP_680086                                                              16    7355
NP_079062                                                              15    9605
NP_078953                                                              15    9605
XP_006726480                                                           15    9605
XP_005248427                                                           15    9605
XP_005248426                                                           15    9605
NP_803875                                                              15    9605

>NP_001121619
          Length = 593

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  NLHLSQLII 9
          NL+LSQLII
Sbjct: 78 NLYLSQLII 86



>NP_000258
          Length = 2818

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  NLHLSQLII 9
          NL+LSQLII
Sbjct: 78 NLYLSQLII 86



>NP_001035957
          Length = 2839

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  NLHLSQLII 9
          NL+LSQLII
Sbjct: 78 NLYLSQLII 86



>XP_006721991
          Length = 1632

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  NLHLSQLII 9
          NL+LSQLII
Sbjct: 78 NLYLSQLII 86



>XP_006721990
          Length = 2823

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  NLHLSQLII 9
          NL+LSQLII
Sbjct: 78 NLYLSQLII 86



>XP_006721989
          Length = 2823

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  NLHLSQLII 9
          NL+LSQLII
Sbjct: 78 NLYLSQLII 86



>XP_005258041
          Length = 2836

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  NLHLSQLII 9
          NL+LSQLII
Sbjct: 78 NLYLSQLII 86



>XP_006721988
          Length = 2846

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  NLHLSQLII 9
          NL+LSQLII
Sbjct: 78 NLYLSQLII 86



>XP_006721987
          Length = 2849

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  NLHLSQLII 9
          NL+LSQLII
Sbjct: 78 NLYLSQLII 86



>XP_006721986
          Length = 2854

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  NLHLSQLII 9
          NL+LSQLII
Sbjct: 65 NLYLSQLII 73



>XP_005258040
          Length = 2857

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  NLHLSQLII 9
          NL+LSQLII
Sbjct: 78 NLYLSQLII 86



>XP_006721985
          Length = 2867

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  NLHLSQLII 9
          NL+LSQLII
Sbjct: 78 NLYLSQLII 86



>NP_689691
          Length = 569

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   LHLSQLII 9
           +HLSQLII
Sbjct: 137 VHLSQLII 144



>XP_005258616
          Length = 526

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   LHLSQLII 9
           +HLSQLII
Sbjct: 94  VHLSQLII 101



>XP_005258615
          Length = 570

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   LHLSQLII 9
           +HLSQLII
Sbjct: 138 VHLSQLII 145



>NP_001153332
          Length = 569

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   LHLSQLII 9
           +HLSQLII
Sbjct: 137 VHLSQLII 144



>NP_001153333
          Length = 569

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   LHLSQLII 9
           +HLSQLII
Sbjct: 137 VHLSQLII 144



>NP_065810
          Length = 4700

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    NLHLSQLI 8
            +LHLSQL+
Sbjct: 3681 SLHLSQLL 3688



>XP_006720684
          Length = 4713

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    NLHLSQLI 8
            +LHLSQL+
Sbjct: 3694 SLHLSQLL 3701



>NP_004517
          Length = 904

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LHLSQLI 8
           LHL+QLI
Sbjct: 300 LHLNQLI 306



>NP_077720
          Length = 126

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2 LHLSQLI 8
         +HLSQL+
Sbjct: 1 MHLSQLL 7



>NP_037438
          Length = 342

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   LHLSQL 7
           LHLSQL
Sbjct: 335 LHLSQL 340



>NP_680086
          Length = 256

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   LHLSQL 7
           LHLSQL
Sbjct: 249 LHLSQL 254



>NP_079062
          Length = 412

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LHLSQLII 9
           +HL QL+I
Sbjct: 249 VHLGQLLI 256



>NP_078953
          Length = 718

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   HLSQLII 9
           HLS LII
Sbjct: 122 HLSSLII 128



>XP_006726480
          Length = 653

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LHLSQLII 9
           +HL QL+I
Sbjct: 494 VHLGQLLI 501



>XP_005248427
          Length = 236

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  LHLSQLII 9
          +HL QL+I
Sbjct: 73 VHLGQLLI 80



>XP_005248426
          Length = 412

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LHLSQLII 9
           +HL QL+I
Sbjct: 249 VHLGQLLI 256



>NP_803875
          Length = 4243

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    NLHLSQLI 8
            +LH SQLI
Sbjct: 2978 DLHQSQLI 2985



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.333    0.147    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 518,336
Number of extensions: 840
Number of successful extensions: 29
Number of sequences better than 10000.0: 29
Number of HSP's gapped: 29
Number of HSP's successfully gapped: 29
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)