Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_073713                                                              19     665
NP_036385                                                              17    3301
NP_001139406                                                           17    3301
NP_001139405                                                           17    3301
NP_071372                                                              16    5631
NP_775899                                                              16    5631
XP_005245872                                                           16    5631
XP_005245870                                                           16    5631
XP_005245869                                                           16    5631
XP_005245866                                                           16    5631
NP_005261                                                              15    9605
XP_006712486                                                           15    9605
XP_006712485                                                           15    9605

>NP_073713
          Length = 985

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   NLGQARVL 8
           NLGQARVL
Sbjct: 335 NLGQARVL 342



>NP_036385
          Length = 596

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   LGQARVLR 9
           +GQA+VLR
Sbjct: 463 IGQAKVLR 470



>NP_001139406
          Length = 540

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   LGQARVLR 9
           +GQA+VLR
Sbjct: 407 IGQAKVLR 414



>NP_001139405
          Length = 590

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   LGQARVLR 9
           +GQA+VLR
Sbjct: 457 IGQAKVLR 464



>NP_071372
          Length = 1180

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NLGQARVLR 9
           ++GQ RVLR
Sbjct: 156 SVGQKRVLR 164



>NP_775899
          Length = 4462

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NLGQARVLR 9
           NLGQ  +LR
Sbjct: 655 NLGQPLILR 663



>XP_005245872
          Length = 1163

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NLGQARVLR 9
           ++GQ RVLR
Sbjct: 156 SVGQKRVLR 164



>XP_005245870
          Length = 1182

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NLGQARVLR 9
           ++GQ RVLR
Sbjct: 156 SVGQKRVLR 164



>XP_005245869
          Length = 1193

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NLGQARVLR 9
           ++GQ RVLR
Sbjct: 156 SVGQKRVLR 164



>XP_005245866
          Length = 1202

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NLGQARVLR 9
           ++GQ RVLR
Sbjct: 156 SVGQKRVLR 164



>NP_005261
          Length = 1586

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    NLGQARVLR 9
            NLG  +V+R
Sbjct: 1462 NLGSCQVMR 1470



>XP_006712486
          Length = 1504

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    NLGQARVLR 9
            NLG  +V+R
Sbjct: 1380 NLGSCQVMR 1388



>XP_006712485
          Length = 1569

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    NLGQARVLR 9
            NLG  +V+R
Sbjct: 1445 NLGSCQVMR 1453



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.329    0.142    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 372,181
Number of extensions: 399
Number of successful extensions: 13
Number of sequences better than 10000.0: 13
Number of HSP's gapped: 13
Number of HSP's successfully gapped: 13
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)