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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000042                                                              19     869
XP_005271621                                                           19     869
XP_006718907                                                           19     869
XP_006718906                                                           19     869
XP_005271619                                                           19     869
XP_005271618                                                           19     869
NP_002462                                                              17    4312
NP_000248                                                              17    4312
XP_005267753                                                           17    4312
XP_006720215                                                           17    4312
XP_006720214                                                           17    4312
XP_005267751                                                           17    4312
NP_001243793                                                           16    5631
NP_001243794                                                           16    5631
NP_055602                                                              16    5631
NP_055796                                                              16    5631
NP_001139693                                                           16    7355
XP_006719087                                                           16    7355
NP_078963                                                              15    9605
NP_056261                                                              15    9605
NP_001186131                                                           15    9605
NP_001186130                                                           15    9605
NP_001003681                                                           15    9605
XP_006724164                                                           15    9605
XP_006724163                                                           15    9605
XP_005247836                                                           15    9605
XP_006713811                                                           15    9605
XP_006713810                                                           15    9605
XP_005247834                                                           15    9605
XP_006711822                                                           15    9605
XP_006711821                                                           15    9605

>NP_000042
          Length = 3056

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   NLESSQKS 8
           NLESSQKS
Sbjct: 471 NLESSQKS 478



>XP_005271621
          Length = 2708

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   NLESSQKS 8
           NLESSQKS
Sbjct: 123 NLESSQKS 130



>XP_006718907
          Length = 3050

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   NLESSQKS 8
           NLESSQKS
Sbjct: 471 NLESSQKS 478



>XP_006718906
          Length = 3056

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   NLESSQKS 8
           NLESSQKS
Sbjct: 471 NLESSQKS 478



>XP_005271619
          Length = 3056

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   NLESSQKS 8
           NLESSQKS
Sbjct: 471 NLESSQKS 478



>XP_005271618
          Length = 3056

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   NLESSQKS 8
           NLESSQKS
Sbjct: 471 NLESSQKS 478



>NP_002462
          Length = 1939

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    LESSQKSA 9
            LESSQK A
Sbjct: 1469 LESSQKEA 1476



>NP_000248
          Length = 1935

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    LESSQKSA 9
            LESSQK A
Sbjct: 1467 LESSQKEA 1474



>XP_005267753
          Length = 1935

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    LESSQKSA 9
            LESSQK A
Sbjct: 1467 LESSQKEA 1474



>XP_006720215
          Length = 1968

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    LESSQKSA 9
            LESSQK A
Sbjct: 1498 LESSQKEA 1505



>XP_006720214
          Length = 1978

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    LESSQKSA 9
            LESSQK A
Sbjct: 1508 LESSQKEA 1515



>XP_005267751
          Length = 1979

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    LESSQKSA 9
            LESSQK A
Sbjct: 1509 LESSQKEA 1516



>NP_001243793
          Length = 970

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NLESSQKSA 9
           NLE  QK+A
Sbjct: 829 NLEGKQKAA 837



>NP_001243794
          Length = 900

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NLESSQKSA 9
           NLE  QK+A
Sbjct: 759 NLEGKQKAA 767



>NP_055602
          Length = 913

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NLESSQKSA 9
           NLE  QK+A
Sbjct: 772 NLEGKQKAA 780



>NP_055796
          Length = 1946

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    NLESSQK 7
            NLESSQ+
Sbjct: 1080 NLESSQR 1086



>NP_001139693
          Length = 340

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  NLESSQK 7
          NLE SQK
Sbjct: 44 NLEESQK 50



>XP_006719087
          Length = 295

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  NLESSQK 7
          NLE SQK
Sbjct: 44 NLEESQK 50



>NP_078963
          Length = 800

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LESSQKS 8
           LES+QKS
Sbjct: 513 LESNQKS 519



>NP_056261
          Length = 2275

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    NLESSQ 6
            NLESSQ
Sbjct: 1962 NLESSQ 1967



>NP_001186131
          Length = 771

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LESSQKS 8
           LES+QKS
Sbjct: 484 LESNQKS 490



>NP_001186130
          Length = 839

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LESSQKS 8
           LES+QKS
Sbjct: 513 LESNQKS 519



>NP_001003681
          Length = 601

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  NLESSQK 7
          +LESSQK
Sbjct: 90 SLESSQK 96



>XP_006724164
          Length = 603

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   NLESSQK 7
           +LESSQK
Sbjct: 133 SLESSQK 139



>XP_006724163
          Length = 644

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   NLESSQK 7
           +LESSQK
Sbjct: 133 SLESSQK 139



>XP_005247836
          Length = 627

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LESSQKS 8
           LES+QKS
Sbjct: 301 LESNQKS 307



>XP_006713811
          Length = 800

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LESSQKS 8
           LES+QKS
Sbjct: 513 LESNQKS 519



>XP_006713810
          Length = 838

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LESSQKS 8
           LES+QKS
Sbjct: 512 LESNQKS 518



>XP_005247834
          Length = 839

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LESSQKS 8
           LES+QKS
Sbjct: 513 LESNQKS 519



>XP_006711822
          Length = 2266

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    NLESSQ 6
            NLESSQ
Sbjct: 1953 NLESSQ 1958



>XP_006711821
          Length = 2301

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    NLESSQ 6
            NLESSQ
Sbjct: 1988 NLESSQ 1993



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.271   0.0893    0.185 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 261,953
Number of extensions: 314
Number of successful extensions: 31
Number of sequences better than 10000.0: 31
Number of HSP's gapped: 31
Number of HSP's successfully gapped: 31
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 18 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.4 bits)
S2: 28 (15.4 bits)