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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_002430                                                              20     390
NP_775797                                                              17    4312
XP_005267429                                                           17    4312
XP_006720123                                                           17    4312
NP_009032                                                              16    5631
NP_001128179                                                           16    5631
XP_006717054                                                           16    5631
XP_006717053                                                           16    5631
XP_005272227                                                           16    5631
NP_004817                                                              16    7355
NP_001277716                                                           16    7355
NP_001005609                                                           16    7355
NP_001005612                                                           16    7355
NP_001390                                                              16    7355
NP_036265                                                              16    7355
XP_006724693                                                           16    7355
NP_073572                                                              15    9605
NP_001073890                                                           15    9605
XP_006722755                                                           15    9605
NP_002064                                                              15    9605

>NP_002430
          Length = 1137

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   NKRSKSIY 8
           NKRSKSIY
Sbjct: 222 NKRSKSIY 229



>NP_775797
          Length = 422

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  NKRSKSIYM 9
          N+R +SIY+
Sbjct: 55 NRRGESIYL 63



>XP_005267429
          Length = 398

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  NKRSKSIYM 9
          N+R +SIY+
Sbjct: 55 NRRGESIYL 63



>XP_006720123
          Length = 425

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  NKRSKSIYM 9
          N+R +SIY+
Sbjct: 55 NRRGESIYL 63



>NP_009032
          Length = 918

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   NKRSKSIY 8
           NKR K IY
Sbjct: 911 NKRVKGIY 918



>NP_001128179
          Length = 918

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   NKRSKSIY 8
           NKR K IY
Sbjct: 911 NKRVKGIY 918



>XP_006717054
          Length = 918

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   NKRSKSIY 8
           NKR K IY
Sbjct: 911 NKRVKGIY 918



>XP_006717053
          Length = 918

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   NKRSKSIY 8
           NKR K IY
Sbjct: 911 NKRVKGIY 918



>XP_005272227
          Length = 918

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   NKRSKSIY 8
           NKR K IY
Sbjct: 911 NKRVKGIY 918



>NP_004817
          Length = 775

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   KRSKSIYM 9
           +RS+SIY+
Sbjct: 723 RRSQSIYL 730



>NP_001277716
          Length = 773

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   KRSKSIYM 9
           +RS+SIY+
Sbjct: 721 RRSQSIYL 728



>NP_001005609
          Length = 389

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NKRSKS 6
           NKRSKS
Sbjct: 157 NKRSKS 162



>NP_001005612
          Length = 386

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NKRSKS 6
           NKRSKS
Sbjct: 157 NKRSKS 162



>NP_001390
          Length = 391

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NKRSKS 6
           NKRSKS
Sbjct: 157 NKRSKS 162



>NP_036265
          Length = 871

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NKRSKSIYM 9
           NK S S+Y+
Sbjct: 819 NKNSHSLYL 827



>XP_006724693
          Length = 388

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NKRSKS 6
           NKRSKS
Sbjct: 157 NKRSKS 162



>NP_073572
          Length = 528

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   RSKSIY 8
           RSKS+Y
Sbjct: 516 RSKSVY 521



>NP_001073890
          Length = 1703

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KRSKSIY 8
           KR+K+IY
Sbjct: 710 KRTKTIY 716



>XP_006722755
          Length = 1722

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KRSKSIY 8
           KR+K+IY
Sbjct: 710 KRTKTIY 716



>NP_002064
          Length = 355

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   NKRSKSIY 8
           NK +K IY
Sbjct: 314 NKETKEIY 321



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.312    0.120    0.303 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 383,898
Number of extensions: 523
Number of successful extensions: 20
Number of sequences better than 10000.0: 20
Number of HSP's gapped: 20
Number of HSP's successfully gapped: 20
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)