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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001257436                                                           22     134
NP_001257437                                                           22     134
NP_006281                                                              22     134
XP_006715618                                                           22     134
XP_005267176                                                           22     134
NP_036250                                                              17    3301
NP_064621                                                              17    3301
XP_005257785                                                           16    5631
XP_005250515                                                           16    5631
XP_005250514                                                           16    5631
NP_002088                                                              16    5631
NP_061877                                                              16    5631
NP_001269952                                                           16    7355
NP_059830                                                              16    7355
XP_006714051                                                           16    7355
NP_005103                                                              16    7355
NP_000279                                                              15    9605
NP_005384                                                              15    9605
NP_061146                                                              15    9605
NP_002883                                                              15    9605
NP_075377                                                              15    9605
NP_075376                                                              15    9605
NP_001156729                                                           15    9605
NP_954872                                                              15    9605
XP_005268022                                                           15    9605
XP_005268021                                                           15    9605
XP_005267076                                                           15    9605

>NP_001257436
          Length = 790

 Score = 21.6 bits (44), Expect =   134
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   NKHFASASG 9
           NKHFA+ASG
Sbjct: 634 NKHFAAASG 642



>NP_001257437
          Length = 790

 Score = 21.6 bits (44), Expect =   134
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   NKHFASASG 9
           NKHFA+ASG
Sbjct: 634 NKHFAAASG 642



>NP_006281
          Length = 790

 Score = 21.6 bits (44), Expect =   134
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   NKHFASASG 9
           NKHFA+ASG
Sbjct: 634 NKHFAAASG 642



>XP_006715618
          Length = 577

 Score = 21.6 bits (44), Expect =   134
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   NKHFASASG 9
           NKHFA+ASG
Sbjct: 421 NKHFAAASG 429



>XP_005267176
          Length = 790

 Score = 21.6 bits (44), Expect =   134
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   NKHFASASG 9
           NKHFA+ASG
Sbjct: 634 NKHFAAASG 642



>NP_036250
          Length = 431

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KHFASAS 8
           KHFAS+S
Sbjct: 335 KHFASSS 341



>NP_064621
          Length = 325

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  NKHFASAS 8
          N+HFA+A+
Sbjct: 40 NRHFAAAT 47



>XP_005257785
          Length = 1887

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KHFASASG 9
           +HF+ ASG
Sbjct: 132 QHFSQASG 139



>XP_005250515
          Length = 1083

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   HFASASG 9
           H+A+ASG
Sbjct: 150 HYAAASG 156



>XP_005250514
          Length = 1089

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   HFASASG 9
           H+A+ASG
Sbjct: 150 HYAAASG 156



>NP_002088
          Length = 365

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   KHFASAS 8
           KHFAS S
Sbjct: 171 KHFASPS 177



>NP_061877
          Length = 1089

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   HFASASG 9
           H+A+ASG
Sbjct: 150 HYAAASG 156



>NP_001269952
          Length = 320

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  KHFASASG 9
          KH A++SG
Sbjct: 69 KHLAASSG 76



>NP_059830
          Length = 606

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NKHFAS 6
           N+HFAS
Sbjct: 544 NEHFAS 549



>XP_006714051
          Length = 480

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NKHFAS 6
           N+HFAS
Sbjct: 418 NEHFAS 423



>NP_005103
          Length = 466

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NKHFAS 6
           N+HFAS
Sbjct: 404 NEHFAS 409



>NP_000279
          Length = 323

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   FASASG 9
           FASASG
Sbjct: 171 FASASG 176



>NP_005384
          Length = 1909

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    FASASG 9
            FASASG
Sbjct: 1133 FASASG 1138



>NP_061146
          Length = 852

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   KHFASASG 9
           +HF  ASG
Sbjct: 710 EHFQEASG 717



>NP_002883
          Length = 1257

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NKHFASAS 8
           NK+F+SA+
Sbjct: 835 NKNFSSAT 842



>NP_075377
          Length = 1188

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NKHFASAS 8
           NK+F+SA+
Sbjct: 835 NKNFSSAT 842



>NP_075376
          Length = 1203

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NKHFASAS 8
           NK+F+SA+
Sbjct: 835 NKNFSSAT 842



>NP_001156729
          Length = 1932

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    FASASG 9
            FASASG
Sbjct: 1156 FASASG 1161



>NP_954872
          Length = 799

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   KHFASASG 9
           +HF  ASG
Sbjct: 657 EHFQEASG 664



>XP_005268022
          Length = 1208

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NKHFASAS 8
           NK+F+SA+
Sbjct: 855 NKNFSSAT 862



>XP_005268021
          Length = 1277

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NKHFASAS 8
           NK+F+SA+
Sbjct: 855 NKNFSSAT 862



>XP_005267076
          Length = 285

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   FASASG 9
           FASASG
Sbjct: 133 FASASG 138



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.307    0.114    0.326 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 513,421
Number of extensions: 679
Number of successful extensions: 27
Number of sequences better than 10000.0: 27
Number of HSP's gapped: 27
Number of HSP's successfully gapped: 27
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)