Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              19     869
NP_001075109                                                           19     869
NP_001165948                                                           16    5631
NP_001164263                                                           16    5631
NP_001164262                                                           16    5631
XP_005264218                                                           16    5631
NP_001005487                                                           16    7355
NP_001933                                                              15    9605
NP_055994                                                              15    9605
NP_001138586                                                           15    9605
NP_001128643                                                           15    9605
NP_001128642                                                           15    9605
NP_114117                                                              15    9605
NP_817123                                                              15    9605
NP_001271266                                                           15    9605
XP_005266332                                                           15    9605
XP_006717155                                                           15    9605
XP_006717154                                                           15    9605
XP_005252009                                                           15    9605
XP_006717151                                                           15    9605
XP_005265873                                                           15    9605
XP_005265872                                                           15    9605
NP_001127925                                                           15    9605
NP_114114                                                              15    9605
>NP_008835
          Length = 4128

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NKELSIGIR 9
           NKELSI IR
Sbjct: 356 NKELSIAIR 364
>NP_001075109
          Length = 4097

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NKELSIGIR 9
           NKELSI IR
Sbjct: 356 NKELSIAIR 364
>NP_001165948
          Length = 423

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  KELSIGIR 9
          KEL++G+R
Sbjct: 61 KELTLGLR 68
>NP_001164263
          Length = 410

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  NKELSIGI 8
          NKEL +GI
Sbjct: 6  NKELILGI 13
>NP_001164262
          Length = 410

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  NKELSIGI 8
          NKEL +GI
Sbjct: 6  NKELILGI 13
>XP_005264218
          Length = 415

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  NKELSIGI 8
          NKEL +GI
Sbjct: 6  NKELILGI 13
>NP_001005487
          Length = 307

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NKELSIGIR 9
           N+E+  GIR
Sbjct: 291 NREMQAGIR 299
>NP_001933
          Length = 1049

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ELSIGIR 9
           +LSIG+R
Sbjct: 346 QLSIGVR 352
>NP_055994
          Length = 1297

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KELSIGIR 9
           KE+S+G R
Sbjct: 867 KEVSVGAR 874
>NP_001138586
          Length = 1236

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KELSIGIR 9
           KE+S+G R
Sbjct: 806 KEVSVGAR 813
>NP_001128643
          Length = 289

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NKELSIGI 8
           NK L+IG+
Sbjct: 203 NKHLAIGL 210
>NP_001128642
          Length = 296

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NKELSIGI 8
           NK L+IG+
Sbjct: 210 NKHLAIGL 217
>NP_114117
          Length = 289

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NKELSIGI 8
           NK L+IG+
Sbjct: 203 NKHLAIGL 210
>NP_817123
          Length = 1040

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NKELSIGIR 9
           N +LSIG++
Sbjct: 344 NIQLSIGVK 352
>NP_001271266
          Length = 1197

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KELSIGIR 9
           KE+S+G R
Sbjct: 767 KEVSVGAR 774
>XP_005266332
          Length = 426

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KELSIGI 8
           K+LS+GI
Sbjct: 406 KQLSVGI 412
>XP_006717155
          Length = 145

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  NKELSIGIR 9
          ++ELS G+R
Sbjct: 24 SEELSTGVR 32
>XP_006717154
          Length = 145

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  NKELSIGIR 9
          ++ELS G+R
Sbjct: 24 SEELSTGVR 32
>XP_005252009
          Length = 358

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  NKELSIGIR 9
          ++ELS G+R
Sbjct: 24 SEELSTGVR 32
>XP_006717151
          Length = 399

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  NKELSIGIR 9
          ++ELS G+R
Sbjct: 24 SEELSTGVR 32
>XP_005265873
          Length = 262

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NKELSIGI 8
           NK L+IG+
Sbjct: 182 NKHLAIGL 189
>XP_005265872
          Length = 303

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NKELSIGI 8
           NK L+IG+
Sbjct: 223 NKHLAIGL 230
>NP_001127925
          Length = 1059

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NKELSIGIR 9
           N +LSIG++
Sbjct: 344 NIQLSIGVK 352
>NP_114114
          Length = 330

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NKELSIGI 8
           NK L+IG+
Sbjct: 250 NKHLAIGL 257
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.314    0.139    0.354 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 488,349
Number of extensions: 820
Number of successful extensions: 24
Number of sequences better than 10000.0: 24
Number of HSP's gapped: 24
Number of HSP's successfully gapped: 24
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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