Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001017981                                                           17    4312
NP_438170                                                              16    5631
NP_077015                                                              16    5631
NP_001244899                                                           16    5631
NP_002961                                                              16    7355
NP_001013682                                                           16    7355
XP_005270918                                                           16    7355
NP_037361                                                              15    9605
NP_079184                                                              15    9605
NP_863651                                                              15    9605
NP_001151                                                              15    9605
NP_863658                                                              15    9605
NP_064536                                                              15    9605
NP_872393                                                              15    9605
XP_005254738                                                           15    9605
XP_006712515                                                           15    9605
XP_006712514                                                           15    9605
XP_006712513                                                           15    9605
XP_006712512                                                           15    9605
XP_006712511                                                           15    9605
XP_006712510                                                           15    9605
XP_006712509                                                           15    9605
XP_005246533                                                           15    9605
XP_006712508                                                           15    9605
NP_115893                                                              15    9605
>NP_001017981
          Length = 377

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NISQMALRP 9
           +ISQ+ LRP
Sbjct: 169 DISQLLLRP 177
>NP_438170
          Length = 434

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   QMALRP 9
           QMALRP
Sbjct: 369 QMALRP 374
>NP_077015
          Length = 1004

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   QMALRP 9
           QMALRP
Sbjct: 606 QMALRP 611
>NP_001244899
          Length = 740

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   QMALRP 9
           QMALRP
Sbjct: 606 QMALRP 611
>NP_002961
          Length = 171

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NISQMALR 8
           N+SQ+A+R
Sbjct: 112 NLSQVAMR 119
>NP_001013682
          Length = 626

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  ISQMALRP 9
          +SQM  RP
Sbjct: 53 VSQMTFRP 60
>XP_005270918
          Length = 592

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  ISQMALRP 9
          +SQM  RP
Sbjct: 53 VSQMTFRP 60
>NP_037361
          Length = 1237

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   NISQMALRP 9
           N+S++ +RP
Sbjct: 594 NLSRLVVRP 602
>NP_079184
          Length = 626

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  NISQMALRP 9
          NI+ ++LRP
Sbjct: 29 NIAYVSLRP 37
>NP_863651
          Length = 1248

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   NISQMALRP 9
           N+S++ +RP
Sbjct: 605 NLSRLVVRP 613
>NP_001151
          Length = 1194

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   NISQMALRP 9
           N+S++ +RP
Sbjct: 594 NLSRLVVRP 602
>NP_863658
          Length = 1205

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   NISQMALRP 9
           N+S++ +RP
Sbjct: 605 NLSRLVVRP 613
>NP_064536
          Length = 370

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   SQMALRP 9
           SQM +RP
Sbjct: 269 SQMVIRP 275
>NP_872393
          Length = 3234

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    NISQMALR 8
            NIS +ALR
Sbjct: 1510 NISSVALR 1517
>XP_005254738
          Length = 472

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  NISQMALRP 9
          NI+ ++LRP
Sbjct: 29 NIAYVSLRP 37
>XP_006712515
          Length = 3232

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    NISQMALR 8
            NIS +ALR
Sbjct: 1508 NISSVALR 1515
>XP_006712514
          Length = 2496

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    NISQMALR 8
            NIS +ALR
Sbjct: 1581 NISSVALR 1588
>XP_006712513
          Length = 3220

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    NISQMALR 8
            NIS +ALR
Sbjct: 1477 NISSVALR 1484
>XP_006712512
          Length = 3237

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    NISQMALR 8
            NIS +ALR
Sbjct: 1513 NISSVALR 1520
>XP_006712511
          Length = 3256

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    NISQMALR 8
            NIS +ALR
Sbjct: 1513 NISSVALR 1520
>XP_006712510
          Length = 3305

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    NISQMALR 8
            NIS +ALR
Sbjct: 1581 NISSVALR 1588
>XP_006712509
          Length = 3319

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    NISQMALR 8
            NIS +ALR
Sbjct: 1576 NISSVALR 1583
>XP_005246533
          Length = 3323

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    NISQMALR 8
            NIS +ALR
Sbjct: 1581 NISSVALR 1588
>XP_006712508
          Length = 3324

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    NISQMALR 8
            NIS +ALR
Sbjct: 1581 NISSVALR 1588
>NP_115893
          Length = 3258

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    NISQMALR 8
            NIS +ALR
Sbjct: 1515 NISSVALR 1522
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.321    0.128    0.346 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 313,560
Number of extensions: 471
Number of successful extensions: 25
Number of sequences better than 10000.0: 25
Number of HSP's gapped: 25
Number of HSP's successfully gapped: 25
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

CANCERTOPE    |     Raghava's Group    |     IMTECH    |     CSIR    |     CRDD    |     GPSR Package