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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_477512                                                              17    4312
XP_005260209                                                           17    4312
NP_001360                                                              16    7355
NP_005227                                                              16    7355
NP_079520                                                              16    7355
NP_878915                                                              16    7355
XP_005248319                                                           16    7355
XP_006712845                                                           16    7355
XP_005264086                                                           16    7355
XP_005264084                                                           16    7355
XP_005264083                                                           16    7355
XP_006712844                                                           16    7355
XP_005264082                                                           16    7355
XP_005264081                                                           16    7355
XP_005264080                                                           16    7355
XP_006712843                                                           16    7355
XP_005264079                                                           16    7355
NP_001275685                                                           15    9605
NP_001028721                                                           15    9605
NP_054892                                                              15    9605

>NP_477512
          Length = 454

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  NISQKMEI 8
          N+SQ ME+
Sbjct: 74 NVSQSMEV 81



>XP_005260209
          Length = 478

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NISQKMEI 8
           N+SQ ME+
Sbjct: 98  NVSQSMEV 105



>NP_001360
          Length = 4624

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    ISQKMEIS 9
            ++QK+EIS
Sbjct: 3795 VTQKLEIS 3802



>NP_005227
          Length = 916

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ISQKMEI 8
           IS+KMEI
Sbjct: 357 ISEKMEI 363



>NP_079520
          Length = 698

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NISQKMEIS 9
           NI+Q+ EIS
Sbjct: 249 NIAQREEIS 257



>NP_878915
          Length = 698

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NISQKMEIS 9
           NI+Q+ EIS
Sbjct: 249 NIAQREEIS 257



>XP_005248319
          Length = 4609

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    ISQKMEIS 9
            ++QK+EIS
Sbjct: 3780 VTQKLEIS 3787



>XP_006712845
          Length = 698

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NISQKMEIS 9
           NI+Q+ EIS
Sbjct: 249 NIAQREEIS 257



>XP_005264086
          Length = 698

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NISQKMEIS 9
           NI+Q+ EIS
Sbjct: 249 NIAQREEIS 257



>XP_005264084
          Length = 698

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NISQKMEIS 9
           NI+Q+ EIS
Sbjct: 249 NIAQREEIS 257



>XP_005264083
          Length = 698

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NISQKMEIS 9
           NI+Q+ EIS
Sbjct: 249 NIAQREEIS 257



>XP_006712844
          Length = 698

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NISQKMEIS 9
           NI+Q+ EIS
Sbjct: 249 NIAQREEIS 257



>XP_005264082
          Length = 698

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NISQKMEIS 9
           NI+Q+ EIS
Sbjct: 249 NIAQREEIS 257



>XP_005264081
          Length = 698

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NISQKMEIS 9
           NI+Q+ EIS
Sbjct: 249 NIAQREEIS 257



>XP_005264080
          Length = 698

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NISQKMEIS 9
           NI+Q+ EIS
Sbjct: 249 NIAQREEIS 257



>XP_006712843
          Length = 891

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NISQKMEIS 9
           NI+Q+ EIS
Sbjct: 449 NIAQREEIS 457



>XP_005264079
          Length = 893

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NISQKMEIS 9
           NI+Q+ EIS
Sbjct: 444 NIAQREEIS 452



>NP_001275685
          Length = 329

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NISQKM 6
           N+SQKM
Sbjct: 122 NVSQKM 127



>NP_001028721
          Length = 329

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NISQKM 6
           N+SQKM
Sbjct: 122 NVSQKM 127



>NP_054892
          Length = 329

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NISQKM 6
           N+SQKM
Sbjct: 122 NVSQKM 127



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.300    0.114    0.252 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 364,000
Number of extensions: 354
Number of successful extensions: 20
Number of sequences better than 10000.0: 20
Number of HSP's gapped: 20
Number of HSP's successfully gapped: 20
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)