Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              18    1482
NP_671714                                                              18    1482
XP_006715890                                                           18    1482
XP_005250160                                                           18    1482
XP_005250159                                                           18    1482
NP_001039                                                              17    2528
NP_443139                                                              17    4312
NP_001254656                                                           17    4312
NP_001139187                                                           17    4312
NP_490595                                                              17    4312
NP_055775                                                              17    4312
XP_006721843                                                           17    4312
XP_006721841                                                           17    4312
XP_006721840                                                           17    4312
XP_005257227                                                           17    4312
XP_006721839                                                           17    4312
XP_006719283                                                           17    4312
XP_006719282                                                           17    4312
XP_006716050                                                           17    4312
XP_005263887                                                           17    4312
NP_001232                                                              17    4312
NP_075556                                                              16    5631
XP_006723676                                                           16    5631
XP_006723675                                                           16    5631
XP_006723674                                                           16    5631
XP_006723673                                                           16    5631
NP_001892                                                              16    7355
NP_060391                                                              16    7355
NP_054799                                                              16    7355
NP_005217                                                              16    7355
XP_005254478                                                           16    7355
NP_002448                                                              15    9605
NP_001181926                                                           15    9605
NP_001014437                                                           15    9605
NP_919226                                                              15    9605
NP_001136105                                                           15    9605
NP_001245330                                                           15    9605
NP_001035049                                                           15    9605
NP_055123                                                              15    9605
NP_258416                                                              15    9605
NP_203134                                                              15    9605
NP_203133                                                              15    9605
NP_150284                                                              15    9605
NP_005705                                                              15    9605
NP_064710                                                              15    9605
NP_001192200                                                           15    9605
XP_006725192                                                           15    9605
XP_006723736                                                           15    9605
XP_005256637                                                           15    9605
XP_005256636                                                           15    9605
XP_005255493                                                           15    9605
XP_006721128                                                           15    9605
XP_005255492                                                           15    9605
XP_006718403                                                           15    9605
XP_006716315                                                           15    9605
>NP_005742
          Length = 3907

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NISCRLQAA 9
            NIS RLQAA
Sbjct: 1845 NISSRLQAA 1853
>NP_671714
          Length = 3899

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NISCRLQAA 9
            NIS RLQAA
Sbjct: 1845 NISSRLQAA 1853
>XP_006715890
          Length = 3860

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NISCRLQAA 9
            NIS RLQAA
Sbjct: 1798 NISSRLQAA 1806
>XP_005250160
          Length = 3939

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NISCRLQAA 9
            NIS RLQAA
Sbjct: 1877 NISSRLQAA 1885
>XP_005250159
          Length = 3952

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NISCRLQAA 9
            NIS RLQAA
Sbjct: 1877 NISSRLQAA 1885
>NP_001039
          Length = 116

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2 ISCRLQAA 9
         +SCRLQ A
Sbjct: 2 LSCRLQCA 9
>NP_443139
          Length = 1078

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SCRLQAA 9
           SCRLQ A
Sbjct: 173 SCRLQGA 179
>NP_001254656
          Length = 525

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ISCRLQAA 9
           I+CR QAA
Sbjct: 518 IACRCQAA 525
>NP_001139187
          Length = 516

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   ISCRLQAA 9
           ISC LQAA
Sbjct: 434 ISCILQAA 441
>NP_490595
          Length = 730

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  ISCRLQAA 9
          +SCR QAA
Sbjct: 34 LSCRQQAA 41
>NP_055775
          Length = 525

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ISCRLQAA 9
           I+CR QAA
Sbjct: 518 IACRCQAA 525
>XP_006721843
          Length = 276

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ISCRLQAA 9
           I+CR QAA
Sbjct: 269 IACRCQAA 276
>XP_006721841
          Length = 524

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ISCRLQAA 9
           I+CR QAA
Sbjct: 517 IACRCQAA 524
>XP_006721840
          Length = 525

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ISCRLQAA 9
           I+CR QAA
Sbjct: 518 IACRCQAA 525
>XP_005257227
          Length = 525

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ISCRLQAA 9
           I+CR QAA
Sbjct: 518 IACRCQAA 525
>XP_006721839
          Length = 525

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ISCRLQAA 9
           I+CR QAA
Sbjct: 518 IACRCQAA 525
>XP_006719283
          Length = 968

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  SCRLQAA 9
          SCRLQ A
Sbjct: 63 SCRLQGA 69
>XP_006719282
          Length = 968

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  SCRLQAA 9
          SCRLQ A
Sbjct: 63 SCRLQGA 69
>XP_006716050
          Length = 516

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   ISCRLQAA 9
           ISC LQAA
Sbjct: 434 ISCILQAA 441
>XP_005263887
          Length = 709

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  ISCRLQAA 9
          +SCR QAA
Sbjct: 34 LSCRQQAA 41
>NP_001232
          Length = 663

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  ISCRLQAA 9
          +SCR QAA
Sbjct: 34 LSCRQQAA 41
>NP_075556
          Length = 1709

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    NISCRL 6
            N+SCRL
Sbjct: 1462 NLSCRL 1467
>XP_006723676
          Length = 1385

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    NISCRL 6
            N+SCRL
Sbjct: 1138 NLSCRL 1143
>XP_006723675
          Length = 1623

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    NISCRL 6
            N+SCRL
Sbjct: 1376 NLSCRL 1381
>XP_006723674
          Length = 1701

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    NISCRL 6
            N+SCRL
Sbjct: 1462 NLSCRL 1467
>XP_006723673
          Length = 1709

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    NISCRL 6
            N+SCRL
Sbjct: 1462 NLSCRL 1467
>NP_001892
          Length = 349

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   NISCRLQ 7
           N SCRL+
Sbjct: 225 NASCRLE 231
>NP_060391
          Length = 608

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   NISCRLQA 8
           NISC++++
Sbjct: 185 NISCKIKS 192
>NP_054799
          Length = 1548

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NISCRL 6
           NISCR+
Sbjct: 959 NISCRV 964
>NP_005217
          Length = 378

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   NISCRLQA 8
           +++CR+QA
Sbjct: 266 DVACRVQA 273
>XP_005254478
          Length = 1548

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NISCRL 6
           NISCR+
Sbjct: 959 NISCRV 964
>NP_002448
          Length = 5179

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1    NISCR 5
            NISCR
Sbjct: 1820 NISCR 1824
>NP_001181926
          Length = 809

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  CRLQAA 9
          CRLQA+
Sbjct: 87 CRLQAS 92
>NP_001014437
          Length = 831

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  CRLQAA 9
          CRLQA+
Sbjct: 87 CRLQAS 92
>NP_919226
          Length = 1209

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   NISCRLQ 7
           NISC+++
Sbjct: 550 NISCKIR 556
>NP_001136105
          Length = 880

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   ISCRLQA 8
           + CRLQA
Sbjct: 214 LHCRLQA 220
>NP_001245330
          Length = 1040

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1    NISCR 5
            NISCR
Sbjct: 1001 NISCR 1005
>NP_001035049
          Length = 1093

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1    NISCR 5
            NISCR
Sbjct: 1054 NISCR 1058
>NP_055123
          Length = 880

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   ISCRLQA 8
           + CRLQA
Sbjct: 214 LHCRLQA 220
>NP_258416
          Length = 252

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  SCRLQA 8
          +CRLQA
Sbjct: 36 ACRLQA 41
>NP_203134
          Length = 252

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  SCRLQA 8
          +CRLQA
Sbjct: 36 ACRLQA 41
>NP_203133
          Length = 307

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  SCRLQA 8
          +CRLQA
Sbjct: 36 ACRLQA 41
>NP_150284
          Length = 79

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  NISCRLQ 7
          + SCRLQ
Sbjct: 25 SFSCRLQ 31
>NP_005705
          Length = 257

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  SCRLQA 8
          +CRLQA
Sbjct: 36 ACRLQA 41
>NP_064710
          Length = 1052

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SCRLQA 8
           SCRL+A
Sbjct: 108 SCRLEA 113
>NP_001192200
          Length = 174

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1  NISCRL 6
          N+SCR+
Sbjct: 38 NVSCRV 43
>XP_006725192
          Length = 769

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  CRLQAA 9
          CRLQA+
Sbjct: 87 CRLQAS 92
>XP_006723736
          Length = 215

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   CRLQAA 9
           CRLQA+
Sbjct: 189 CRLQAS 194
>XP_005256637
          Length = 879

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   ISCRLQA 8
           + CRLQA
Sbjct: 214 LHCRLQA 220
>XP_005256636
          Length = 880

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   ISCRLQA 8
           + CRLQA
Sbjct: 214 LHCRLQA 220
>XP_005255493
          Length = 959

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SCRLQA 8
           SCRL+A
Sbjct: 108 SCRLEA 113
>XP_006721128
          Length = 985

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SCRLQA 8
           SCRL+A
Sbjct: 108 SCRLEA 113
>XP_005255492
          Length = 1026

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SCRLQA 8
           SCRL+A
Sbjct: 108 SCRLEA 113
>XP_006718403
          Length = 769

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  CRLQAA 9
          CRLQA+
Sbjct: 87 CRLQAS 92
>XP_006716315
          Length = 442

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   NISCR 5
           NISCR
Sbjct: 348 NISCR 352
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.328    0.126    0.374 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 618,610
Number of extensions: 603
Number of successful extensions: 55
Number of sequences better than 10000.0: 55
Number of HSP's gapped: 55
Number of HSP's successfully gapped: 55
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)

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