Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006209                                                              20     390
XP_006713721                                                           20     390
NP_001714                                                              17    3301
XP_005249383                                                           17    3301
NP_001274268                                                           17    4312
NP_001073138                                                           17    4312
NP_112187                                                              17    4312
NP_001274266                                                           17    4312
NP_001274267                                                           17    4312
NP_001124322                                                           17    4312
NP_001124321                                                           17    4312
NP_037490                                                              17    4312
NP_057373                                                              17    4312
NP_001186068                                                           16    7355
NP_067032                                                              16    7355
NP_001186067                                                           16    7355
NP_055523                                                              16    7355
NP_056150                                                              16    7355
NP_004661                                                              16    7355
NP_001015880                                                           16    7355
XP_006719769                                                           16    7355
XP_006719370                                                           16    7355
XP_006719369                                                           16    7355
XP_006719368                                                           16    7355
XP_006712965                                                           16    7355
NP_009044                                                              15    9605
NP_001156806                                                           15    9605
NP_620135                                                              15    9605
NP_001156807                                                           15    9605
NP_085125                                                              15    9605
NP_001008781                                                           15    9605
NP_001363                                                              15    9605
NP_004653                                                              15    9605
>NP_006209
          Length = 1068

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   ILNREIGF 9
           ILNREIGF
Sbjct: 112 ILNREIGF 119
>XP_006713721
          Length = 1068

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   ILNREIGF 9
           ILNREIGF
Sbjct: 112 ILNREIGF 119
>NP_001714
          Length = 2649

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    NILNREIG 8
            NI+N+E+G
Sbjct: 2545 NIINKEMG 2552
>XP_005249383
          Length = 3186

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    NILNREIG 8
            NI+N+E+G
Sbjct: 3082 NIINKEMG 3089
>NP_001274268
          Length = 337

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NILNREIG 8
           NIL R+IG
Sbjct: 103 NILRRQIG 110
>NP_001073138
          Length = 337

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NILNREIG 8
           NIL R+IG
Sbjct: 103 NILRRQIG 110
>NP_112187
          Length = 337

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NILNREIG 8
           NIL R+IG
Sbjct: 103 NILRRQIG 110
>NP_001274266
          Length = 337

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NILNREIG 8
           NIL R+IG
Sbjct: 103 NILRRQIG 110
>NP_001274267
          Length = 337

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NILNREIG 8
           NIL R+IG
Sbjct: 103 NILRRQIG 110
>NP_001124322
          Length = 341

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NILNREIG 8
           NIL R+IG
Sbjct: 104 NILRRQIG 111
>NP_001124321
          Length = 341

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NILNREIG 8
           NIL R+IG
Sbjct: 104 NILRRQIG 111
>NP_037490
          Length = 663

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NILNREIG 8
           N+L RE+G
Sbjct: 552 NVLKRELG 559
>NP_057373
          Length = 341

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NILNREIG 8
           NIL R+IG
Sbjct: 104 NILRRQIG 111
>NP_001186068
          Length = 1024

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  NILNRE 6
          N+LNRE
Sbjct: 31 NVLNRE 36
>NP_067032
          Length = 1024

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  NILNRE 6
          N+LNRE
Sbjct: 31 NVLNRE 36
>NP_001186067
          Length = 1024

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  NILNRE 6
          N+LNRE
Sbjct: 31 NVLNRE 36
>NP_055523
          Length = 2209

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   NILNREI 7
           N+LN+EI
Sbjct: 855 NLLNKEI 861
>NP_056150
          Length = 2210

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2    ILNREIGF 9
            I+NR++G+
Sbjct: 1167 IMNRKLGY 1174
>NP_004661
          Length = 614

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  LNREIGF 9
          LNR +GF
Sbjct: 85 LNRNLGF 91
>NP_001015880
          Length = 619

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  LNREIGF 9
          LNR +GF
Sbjct: 85 LNRNLGF 91
>XP_006719769
          Length = 2209

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   NILNREI 7
           N+LN+EI
Sbjct: 855 NLLNKEI 861
>XP_006719370
          Length = 2076

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2    ILNREIGF 9
            I+NR++G+
Sbjct: 1033 IMNRKLGY 1040
>XP_006719369
          Length = 2200

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2    ILNREIGF 9
            I+NR++G+
Sbjct: 1157 IMNRKLGY 1164
>XP_006719368
          Length = 2209

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2    ILNREIGF 9
            I+NR++G+
Sbjct: 1166 IMNRKLGY 1173
>XP_006712965
          Length = 423

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  NILNREIGF 9
          N+L R+I F
Sbjct: 2  NLLQRQIAF 10
>NP_009044
          Length = 1943

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  NILNREIG 8
          N+L RE G
Sbjct: 35 NLLEREFG 42
>NP_001156806
          Length = 752

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   NILNREIGF 9
           ++++RE GF
Sbjct: 273 SVISREYGF 281
>NP_620135
          Length = 730

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   NILNREIGF 9
           ++++RE GF
Sbjct: 273 SVISREYGF 281
>NP_001156807
          Length = 730

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   NILNREIGF 9
           ++++RE GF
Sbjct: 273 SVISREYGF 281
>NP_085125
          Length = 504

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   LNREIG 8
           LNRE+G
Sbjct: 332 LNRELG 337
>NP_001008781
          Length = 4557

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   ILNREI 7
           ILNREI
Sbjct: 116 ILNREI 121
>NP_001363
          Length = 4486

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    NILNREI 7
            NIL REI
Sbjct: 4263 NILTREI 4269
>NP_004653
          Length = 798

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NILNREI 7
           NIL REI
Sbjct: 575 NILTREI 581
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.329    0.155    0.446 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 450,491
Number of extensions: 720
Number of successful extensions: 33
Number of sequences better than 10000.0: 33
Number of HSP's gapped: 33
Number of HSP's successfully gapped: 33
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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