Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000305                                                              19     869
XP_006717990                                                           19     869
XP_006717989                                                           19     869
NP_057196                                                              17    2528
NP_001240766                                                           17    2528
NP_065979                                                              17    4312
NP_001243208                                                           17    4312
NP_062537                                                              17    4312
NP_065934                                                              17    4312
XP_006719809                                                           17    4312
XP_005266240                                                           17    4312
XP_005266239                                                           17    4312
XP_006719808                                                           17    4312
XP_006719807                                                           17    4312
NP_612567                                                              16    5631
NP_003257                                                              16    5631
NP_612564                                                              16    5631
NP_057349                                                              16    5631
NP_008887                                                              16    5631
NP_060572                                                              16    5631
NP_001257426                                                           16    5631
NP_037523                                                              16    5631
XP_006723880                                                           16    5631
XP_005260504                                                           16    5631
XP_006723879                                                           16    5631
XP_006714661                                                           16    5631
XP_006714660                                                           16    5631
NP_006437                                                              16    7355
NP_689821                                                              16    7355
NP_079493                                                              16    7355
NP_001230280                                                           16    7355
NP_057202                                                              16    7355
NP_001129666                                                           16    7355
NP_001129665                                                           16    7355
NP_001186253                                                           16    7355
NP_067645                                                              16    7355
NP_940882                                                              16    7355
XP_005250069                                                           16    7355
XP_005250068                                                           16    7355
XP_005250067                                                           16    7355
XP_005267207                                                           16    7355
XP_005273137                                                           16    7355
NP_055744                                                              16    7355
NP_057347                                                              15    9605
NP_001009562                                                           15    9605
XP_005259630                                                           15    9605
NP_001129670                                                           15    9605
NP_001129669                                                           15    9605
>NP_000305
          Length = 403

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  NIIAMGFHA 9
          NIIAMGF A
Sbjct: 31 NIIAMGFPA 39
>XP_006717990
          Length = 175

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  NIIAMGFHA 9
          NIIAMGF A
Sbjct: 31 NIIAMGFPA 39
>XP_006717989
          Length = 388

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  NIIAMGFHA 9
          NIIAMGF A
Sbjct: 31 NIIAMGFPA 39
>NP_057196
          Length = 805

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NIIAMGFH 8
           +I+A+GFH
Sbjct: 397 SIVAVGFH 404
>NP_001240766
          Length = 758

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NIIAMGFH 8
           +I+A+GFH
Sbjct: 350 SIVAVGFH 357
>NP_065979
          Length = 1215

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   NIIAMGFH 8
           N++ +GFH
Sbjct: 221 NVLQVGFH 228
>NP_001243208
          Length = 566

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1  NIIAMGFH 8
          N+I++G+H
Sbjct: 82 NLISVGYH 89
>NP_062537
          Length = 533

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1  NIIAMGFH 8
          N+I++G+H
Sbjct: 49 NLISVGYH 56
>NP_065934
          Length = 1185

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   NIIAMGFH 8
           N++ +GFH
Sbjct: 194 NVLQVGFH 201
>XP_006719809
          Length = 513

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1  NIIAMGFH 8
          N+I++G+H
Sbjct: 29 NLISVGYH 36
>XP_005266240
          Length = 513

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1  NIIAMGFH 8
          N+I++G+H
Sbjct: 29 NLISVGYH 36
>XP_005266239
          Length = 513

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1  NIIAMGFH 8
          N+I++G+H
Sbjct: 29 NLISVGYH 36
>XP_006719808
          Length = 513

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1  NIIAMGFH 8
          N+I++G+H
Sbjct: 29 NLISVGYH 36
>XP_006719807
          Length = 513

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1  NIIAMGFH 8
          N+I++G+H
Sbjct: 29 NLISVGYH 36
>NP_612567
          Length = 639

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   NIIAMGFH 8
           NII  GFH
Sbjct: 521 NIIHEGFH 528
>NP_003257
          Length = 799

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   NIIAMGFH 8
           NII  GFH
Sbjct: 681 NIIHEGFH 688
>NP_612564
          Length = 839

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   NIIAMGFH 8
           NII  GFH
Sbjct: 721 NIIHEGFH 728
>NP_057349
          Length = 697

 Score = 16.2 bits (30), Expect =  5631
 Identities = 3/8 (37%), Positives = 8/8 (100%)

Query: 1  NIIAMGFH 8
          N++++G+H
Sbjct: 43 NLVSVGYH 50
>NP_008887
          Length = 659

 Score = 16.2 bits (30), Expect =  5631
 Identities = 3/8 (37%), Positives = 8/8 (100%)

Query: 1  NIIAMGFH 8
          N++++G+H
Sbjct: 43 NLVSVGYH 50
>NP_060572
          Length = 680

 Score = 16.2 bits (30), Expect =  5631
 Identities = 3/8 (37%), Positives = 8/8 (100%)

Query: 1  NIIAMGFH 8
          N++++G+H
Sbjct: 45 NLVSVGYH 52
>NP_001257426
          Length = 679

 Score = 16.2 bits (30), Expect =  5631
 Identities = 3/8 (37%), Positives = 8/8 (100%)

Query: 1  NIIAMGFH 8
          N++++G+H
Sbjct: 44 NLVSVGYH 51
>NP_037523
          Length = 866

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   AMGFHA 9
           +MGFHA
Sbjct: 483 SMGFHA 488
>XP_006723880
          Length = 680

 Score = 16.2 bits (30), Expect =  5631
 Identities = 3/8 (37%), Positives = 8/8 (100%)

Query: 1  NIIAMGFH 8
          N++++G+H
Sbjct: 45 NLVSVGYH 52
>XP_005260504
          Length = 680

 Score = 16.2 bits (30), Expect =  5631
 Identities = 3/8 (37%), Positives = 8/8 (100%)

Query: 1  NIIAMGFH 8
          N++++G+H
Sbjct: 45 NLVSVGYH 52
>XP_006723879
          Length = 703

 Score = 16.2 bits (30), Expect =  5631
 Identities = 3/8 (37%), Positives = 8/8 (100%)

Query: 1  NIIAMGFH 8
          N++++G+H
Sbjct: 68 NLVSVGYH 75
>XP_006714661
          Length = 486

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   AMGFHA 9
           +MGFHA
Sbjct: 103 SMGFHA 108
>XP_006714660
          Length = 774

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   AMGFHA 9
           +MGFHA
Sbjct: 483 SMGFHA 488
>NP_006437
          Length = 691

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   IAMGFHA 9
           +A+GFH+
Sbjct: 570 LALGFHS 576
>NP_689821
          Length = 451

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5   MGFHA 9
           MGFHA
Sbjct: 244 MGFHA 248
>NP_079493
          Length = 246

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 2   IIAMGFH 8
           ++AM FH
Sbjct: 177 VVAMSFH 183
>NP_001230280
          Length = 639

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   IIAMGFH 8
           ++AMG+H
Sbjct: 258 MLAMGYH 264
>NP_057202
          Length = 625

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   IIAMGFH 8
           ++AMG+H
Sbjct: 258 MLAMGYH 264
>NP_001129666
          Length = 710

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4   AMGFH 8
           AMGFH
Sbjct: 664 AMGFH 668
>NP_001129665
          Length = 710

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4   AMGFH 8
           AMGFH
Sbjct: 664 AMGFH 668
>NP_001186253
          Length = 742

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  NIIAMGFHA 9
          N++A+G+ A
Sbjct: 43 NLVAVGYQA 51
>NP_067645
          Length = 532

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  NIIAMGFHA 9
          N++A+G+ A
Sbjct: 43 NLVAVGYQA 51
>NP_940882
          Length = 731

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  NIIAMGFHA 9
          N++A+G+ A
Sbjct: 43 NLVAVGYQA 51
>XP_005250069
          Length = 590

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   IIAMGFH 8
           ++AMG+H
Sbjct: 258 MLAMGYH 264
>XP_005250068
          Length = 615

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   IIAMGFH 8
           ++AMG+H
Sbjct: 234 MLAMGYH 240
>XP_005250067
          Length = 638

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   IIAMGFH 8
           ++AMG+H
Sbjct: 257 MLAMGYH 263
>XP_005267207
          Length = 246

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 2   IIAMGFH 8
           ++AM FH
Sbjct: 177 VVAMSFH 183
>XP_005273137
          Length = 466

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5   MGFHA 9
           MGFHA
Sbjct: 259 MGFHA 263
>NP_055744
          Length = 710

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4   AMGFH 8
           AMGFH
Sbjct: 664 AMGFH 668
>NP_057347
          Length = 493

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   NIIAMGFH 8
           N++A+G H
Sbjct: 242 NLVAVGTH 249
>NP_001009562
          Length = 640

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   IAMGFHA 9
           +A+GFH+
Sbjct: 523 LAIGFHS 529
>XP_005259630
          Length = 496

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   NIIAMGFH 8
           N++A+G H
Sbjct: 242 NLVAVGTH 249
>NP_001129670
          Length = 496

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   NIIAMGFH 8
           N++A+G H
Sbjct: 242 NLVAVGTH 249
>NP_001129669
          Length = 404

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   NIIAMGFH 8
           N++A+G H
Sbjct: 153 NLVAVGTH 160
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.339    0.145    0.453 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 492,199
Number of extensions: 714
Number of successful extensions: 48
Number of sequences better than 10000.0: 48
Number of HSP's gapped: 48
Number of HSP's successfully gapped: 48
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.5 bits)
S2: 28 (15.4 bits)

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