Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_115803                                                              20     299
NP_705838                                                              16    5631
NP_001371                                                              16    5631
NP_001277152                                                           16    5631
NP_859067                                                              16    5631
NP_573420                                                              16    5631
NP_573419                                                              16    5631
NP_573418                                                              16    5631
NP_006042                                                              16    5631
NP_001035519                                                           16    5631
XP_005266415                                                           16    5631
XP_005266414                                                           16    5631
XP_006717746                                                           16    5631
XP_006717745                                                           16    5631
NP_004454                                                              16    7355
NP_001018066                                                           15    9605
NP_003374                                                              15    9605
NP_055778                                                              15    9605
NP_001093592                                                           15    9605
NP_001034485                                                           15    9605
XP_005259577                                                           15    9605
XP_005259576                                                           15    9605
XP_006716928                                                           15    9605
XP_006716927                                                           15    9605
>NP_115803
          Length = 1156

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   NIDLGRQSQ 9
           NIDLG+QSQ
Sbjct: 341 NIDLGQQSQ 349
>NP_705838
          Length = 4069

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    DLGRQS 8
            DLGRQS
Sbjct: 1644 DLGRQS 1649
>NP_001371
          Length = 1865

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NIDLGRQSQ 9
           NID+ RQ++
Sbjct: 208 NIDINRQAK 216
>NP_001277152
          Length = 1886

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NIDLGRQSQ 9
           NID+ RQ++
Sbjct: 208 NIDINRQAK 216
>NP_859067
          Length = 272

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NIDLGRQSQ 9
           N+DLG Q +
Sbjct: 105 NLDLGEQRE 113
>NP_573420
          Length = 484

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   DLGRQS 8
           DLGRQS
Sbjct: 377 DLGRQS 382
>NP_573419
          Length = 486

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   DLGRQS 8
           DLGRQS
Sbjct: 379 DLGRQS 384
>NP_573418
          Length = 491

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   DLGRQS 8
           DLGRQS
Sbjct: 384 DLGRQS 389
>NP_006042
          Length = 493

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   DLGRQS 8
           DLGRQS
Sbjct: 386 DLGRQS 391
>NP_001035519
          Length = 1159

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    NIDLGRQS 8
            N DLGR+S
Sbjct: 1125 NRDLGRES 1132
>XP_005266415
          Length = 1158

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    NIDLGRQS 8
            N DLGR+S
Sbjct: 1124 NRDLGRES 1131
>XP_005266414
          Length = 1159

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    NIDLGRQS 8
            N DLGR+S
Sbjct: 1125 NRDLGRES 1132
>XP_006717746
          Length = 950

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NIDLGRQSQ 9
           NID+ RQ++
Sbjct: 208 NIDINRQAK 216
>XP_006717745
          Length = 1046

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NIDLGRQSQ 9
           NID+ RQ++
Sbjct: 208 NIDINRQAK 216
>NP_004454
          Length = 961

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   NIDLGRQS 8
           N+DLG+++
Sbjct: 717 NVDLGKRA 724
>NP_001018066
          Length = 845

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NIDLGRQS 8
           +ID+GR S
Sbjct: 818 SIDIGRHS 825
>NP_003374
          Length = 873

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NIDLGRQS 8
           +ID+GR S
Sbjct: 846 SIDIGRHS 853
>NP_055778
          Length = 1366

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    DLGRQSQ 9
            ++GRQSQ
Sbjct: 1097 NIGRQSQ 1103
>NP_001093592
          Length = 1309

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    DLGRQSQ 9
            ++GRQSQ
Sbjct: 1040 NIGRQSQ 1046
>NP_001034485
          Length = 716

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1  NIDLGR 6
          N+D+GR
Sbjct: 56 NVDMGR 61
>XP_005259577
          Length = 1366

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    DLGRQSQ 9
            ++GRQSQ
Sbjct: 1097 NIGRQSQ 1103
>XP_005259576
          Length = 1366

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    DLGRQSQ 9
            ++GRQSQ
Sbjct: 1097 NIGRQSQ 1103
>XP_006716928
          Length = 850

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NIDLGRQS 8
           +ID+GR S
Sbjct: 823 SIDIGRHS 830
>XP_006716927
          Length = 878

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NIDLGRQS 8
           +ID+GR S
Sbjct: 851 SIDIGRHS 858
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.311    0.132    0.349 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 477,818
Number of extensions: 753
Number of successful extensions: 24
Number of sequences better than 10000.0: 24
Number of HSP's gapped: 24
Number of HSP's successfully gapped: 24
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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