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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              20     509
NP_671714                                                              20     509
XP_006715890                                                           20     509
XP_005250160                                                           20     509
XP_005250159                                                           20     509
NP_000466                                                              15    9605
NP_958843                                                              15    9605
NP_660201                                                              15    9605
XP_006719710                                                           15    9605
XP_006719709                                                           15    9605
XP_006719708                                                           15    9605
XP_006719707                                                           15    9605
XP_006719706                                                           15    9605
XP_005274171                                                           15    9605
XP_006718703                                                           15    9605

>NP_005742
          Length = 3907

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NIASSKQAH 9
            N ASSKQAH
Sbjct: 1470 NTASSKQAH 1478



>NP_671714
          Length = 3899

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NIASSKQAH 9
            N ASSKQAH
Sbjct: 1470 NTASSKQAH 1478



>XP_006715890
          Length = 3860

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NIASSKQAH 9
            N ASSKQAH
Sbjct: 1470 NTASSKQAH 1478



>XP_005250160
          Length = 3939

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NIASSKQAH 9
            N ASSKQAH
Sbjct: 1470 NTASSKQAH 1478



>XP_005250159
          Length = 3952

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NIASSKQAH 9
            N ASSKQAH
Sbjct: 1470 NTASSKQAH 1478



>NP_000466
          Length = 470

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   IASSKQAH 9
           + SSKQ H
Sbjct: 150 LTSSKQTH 157



>NP_958843
          Length = 684

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NIASSKQ 7
           NIA SKQ
Sbjct: 269 NIAKSKQ 275



>NP_660201
          Length = 275

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  ASSKQAH 9
          ASS+Q+H
Sbjct: 91 ASSRQSH 97



>XP_006719710
          Length = 523

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NIASSKQ 7
           NIA SKQ
Sbjct: 108 NIAKSKQ 114



>XP_006719709
          Length = 632

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NIASSKQ 7
           NIA SKQ
Sbjct: 269 NIAKSKQ 275



>XP_006719708
          Length = 634

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NIASSKQ 7
           NIA SKQ
Sbjct: 269 NIAKSKQ 275



>XP_006719707
          Length = 682

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NIASSKQ 7
           NIA SKQ
Sbjct: 269 NIAKSKQ 275



>XP_006719706
          Length = 684

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NIASSKQ 7
           NIA SKQ
Sbjct: 269 NIAKSKQ 275



>XP_005274171
          Length = 182

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  ASSKQAH 9
          ASS+Q+H
Sbjct: 91 ASSRQSH 97



>XP_006718703
          Length = 227

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  ASSKQAH 9
          ASS+Q+H
Sbjct: 91 ASSRQSH 97



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.298    0.102    0.251 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 310,396
Number of extensions: 342
Number of successful extensions: 15
Number of sequences better than 10000.0: 15
Number of HSP's gapped: 15
Number of HSP's successfully gapped: 15
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)