Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_002430                                                              21     229
NP_001001419                                                           17    2528
NP_005894                                                              17    2528
NP_001001420                                                           17    2528
NP_001243888                                                           17    3301
NP_001277                                                              17    3301
NP_001756                                                              17    3301
XP_005245636                                                           17    3301
NP_003319                                                              17    4312
NP_803238                                                              17    4312
NP_789787                                                              16    5631
NP_001094891                                                           16    5631
NP_001185787                                                           16    7355
NP_001238903                                                           16    7355
NP_694539                                                              16    7355
NP_055696                                                              16    7355
NP_001180404                                                           16    7355
NP_001258745                                                           16    7355
NP_001238902                                                           16    7355
NP_001129101                                                           16    7355
XP_005275603                                                           16    7355
XP_005272864                                                           16    7355
XP_005274912                                                           16    7355
XP_006718153                                                           16    7355
XP_005249108                                                           16    7355
XP_006715011                                                           16    7355
NP_000227                                                              15    9605
NP_694996                                                              15    9605
NP_056132                                                              15    9605
NP_001269719                                                           15    9605
NP_055491                                                              15    9605
NP_001265227                                                           15    9605
NP_001018494                                                           15    9605
XP_006720565                                                           15    9605
XP_005254431                                                           15    9605
XP_006720564                                                           15    9605
XP_005254429                                                           15    9605
XP_005262667                                                           15    9605
XP_005262666                                                           15    9605
XP_006712037                                                           15    9605
XP_006712036                                                           15    9605
XP_006712035                                                           15    9605
XP_005262664                                                           15    9605
>NP_002430
          Length = 1137

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   NHAFQAVTE 9
           +HAFQAVTE
Sbjct: 466 SHAFQAVTE 474
>NP_001001419
          Length = 465

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NHAFQAVTE 9
           NH F+AV E
Sbjct: 397 NHGFEAVYE 405
>NP_005894
          Length = 465

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NHAFQAVTE 9
           NH F+AV E
Sbjct: 397 NHGFEAVYE 405
>NP_001001420
          Length = 465

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NHAFQAVTE 9
           NH F+AV E
Sbjct: 397 NHGFEAVYE 405
>NP_001243888
          Length = 847

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NHAFQAVTE 9
           +HAF  VTE
Sbjct: 608 HHAFPVVTE 616
>NP_001277
          Length = 869

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NHAFQAVTE 9
           +HAF  VTE
Sbjct: 630 HHAFPVVTE 638
>NP_001756
          Length = 333

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NHAFQAVTE 9
           NH ++ VTE
Sbjct: 167 NHQYEGVTE 175
>XP_005245636
          Length = 346

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NHAFQAVTE 9
           NH ++ VTE
Sbjct: 167 NHQYEGVTE 175
>NP_003319
          Length = 527

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   HAFQAVTE 9
           HAFQ++ E
Sbjct: 219 HAFQSIPE 226
>NP_803238
          Length = 189

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NHAFQAVTE 9
           N+AFQA+ E
Sbjct: 92  NNAFQALRE 100
>NP_789787
          Length = 333

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1  NHAFQAV 7
          NH++QA+
Sbjct: 89 NHSYQAI 95
>NP_001094891
          Length = 1022

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   HAFQAVTE 9
           H F+AVT+
Sbjct: 958 HVFEAVTK 965
>NP_001185787
          Length = 227

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  FQAVTE 9
          FQAVTE
Sbjct: 75 FQAVTE 80
>NP_001238903
          Length = 243

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  AFQAVTE 9
          AFQA+T+
Sbjct: 45 AFQAITD 51
>NP_694539
          Length = 769

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   HAFQAVTE 9
           H F+++TE
Sbjct: 320 HLFESITE 327
>NP_055696
          Length = 1040

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NHAFQAVT 8
           N AF+AVT
Sbjct: 860 NTAFEAVT 867
>NP_001180404
          Length = 738

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   HAFQAVTE 9
           H F+++TE
Sbjct: 320 HLFESITE 327
>NP_001258745
          Length = 1040

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NHAFQAVT 8
           N AF+AVT
Sbjct: 860 NTAFEAVT 867
>NP_001238902
          Length = 243

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  AFQAVTE 9
          AFQA+T+
Sbjct: 45 AFQAITD 51
>NP_001129101
          Length = 780

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   HAFQAVTE 9
           H F+++TE
Sbjct: 320 HLFESITE 327
>XP_005275603
          Length = 275

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  FQAVTE 9
          FQAVTE
Sbjct: 75 FQAVTE 80
>XP_005272864
          Length = 275

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  FQAVTE 9
          FQAVTE
Sbjct: 75 FQAVTE 80
>XP_005274912
          Length = 275

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  FQAVTE 9
          FQAVTE
Sbjct: 75 FQAVTE 80
>XP_006718153
          Length = 1040

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NHAFQAVT 8
           N AF+AVT
Sbjct: 860 NTAFEAVT 867
>XP_005249108
          Length = 275

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  FQAVTE 9
          FQAVTE
Sbjct: 75 FQAVTE 80
>XP_006715011
          Length = 243

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  AFQAVTE 9
          AFQA+T+
Sbjct: 45 AFQAITD 51
>NP_000227
          Length = 499

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   HAFQAVTE 9
           H F A+T+
Sbjct: 266 HGFNAITQ 273
>NP_694996
          Length = 2526

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    NHAFQAVTE 9
            N  FQ VTE
Sbjct: 2189 NDGFQFVTE 2197
>NP_056132
          Length = 1064

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1 NHAFQAV 7
         NH FQA+
Sbjct: 2 NHDFQAL 8
>NP_001269719
          Length = 985

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1 NHAFQAV 7
         NH FQA+
Sbjct: 2 NHDFQAL 8
>NP_055491
          Length = 1186

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   NHAFQAV 7
           NH FQA+
Sbjct: 129 NHDFQAL 135
>NP_001265227
          Length = 2614

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    NHAFQAVTE 9
            N  FQ VTE
Sbjct: 2189 NDGFQFVTE 2197
>NP_001018494
          Length = 1188

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   NHAFQAV 7
           NH FQA+
Sbjct: 129 NHDFQAL 135
>XP_006720565
          Length = 452

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   HAFQAVTE 9
           H F A+T+
Sbjct: 219 HGFNAITQ 226
>XP_005254431
          Length = 478

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   HAFQAVTE 9
           H F A+T+
Sbjct: 245 HGFNAITQ 252
>XP_006720564
          Length = 499

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   HAFQAVTE 9
           H F A+T+
Sbjct: 266 HGFNAITQ 273
>XP_005254429
          Length = 499

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   HAFQAVTE 9
           H F A+T+
Sbjct: 266 HGFNAITQ 273
>XP_005262667
          Length = 985

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1 NHAFQAV 7
         NH FQA+
Sbjct: 2 NHDFQAL 8
>XP_005262666
          Length = 987

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1 NHAFQAV 7
         NH FQA+
Sbjct: 2 NHDFQAL 8
>XP_006712037
          Length = 1066

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1 NHAFQAV 7
         NH FQA+
Sbjct: 2 NHDFQAL 8
>XP_006712036
          Length = 1066

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1 NHAFQAV 7
         NH FQA+
Sbjct: 2 NHDFQAL 8
>XP_006712035
          Length = 1066

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1 NHAFQAV 7
         NH FQA+
Sbjct: 2 NHDFQAL 8
>XP_005262664
          Length = 1066

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1 NHAFQAV 7
         NH FQA+
Sbjct: 2 NHDFQAL 8
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.316    0.118    0.333 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 343,300
Number of extensions: 436
Number of successful extensions: 43
Number of sequences better than 10000.0: 43
Number of HSP's gapped: 43
Number of HSP's successfully gapped: 43
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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