Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_061987                                                              17    2528
XP_006717209                                                           17    2528
XP_006717208                                                           17    2528
XP_006717207                                                           17    2528
NP_060516                                                              17    4312
NP_060393                                                              17    4312
NP_839955                                                              16    5631
NP_653184                                                              16    5631
NP_001034451                                                           16    5631
NP_001020095                                                           16    5631
XP_005265807                                                           16    5631
XP_006714105                                                           16    5631
XP_005265805                                                           16    5631
XP_005265804                                                           16    5631
NP_060731                                                              16    5631
NP_852478                                                              16    7355
NP_001949                                                              16    7355
NP_004789                                                              16    7355
NP_004791                                                              16    7355
NP_005774                                                              16    7355
NP_001243556                                                           15    9605
NP_003948                                                              15    9605
NP_001243559                                                           15    9605
NP_001243558                                                           15    9605
NP_003595                                                              15    9605
NP_001269147                                                           15    9605
NP_001093143                                                           15    9605
NP_001093142                                                           15    9605
NP_001093141                                                           15    9605
NP_001093140                                                           15    9605
XP_005262277                                                           15    9605
XP_006724776                                                           15    9605
XP_005253277                                                           15    9605
XP_006718442                                                           15    9605
XP_006718441                                                           15    9605
XP_006718440                                                           15    9605
XP_006718439                                                           15    9605
XP_005253275                                                           15    9605
XP_006718438                                                           15    9605
XP_006718437                                                           15    9605
NP_060499                                                              15    9605
>NP_061987
          Length = 900

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   FVNVEKKS 9
           FVN+ KKS
Sbjct: 634 FVNINKKS 641
>XP_006717209
          Length = 812

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   FVNVEKKS 9
           FVN+ KKS
Sbjct: 634 FVNINKKS 641
>XP_006717208
          Length = 838

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   FVNVEKKS 9
           FVN+ KKS
Sbjct: 634 FVNINKKS 641
>XP_006717207
          Length = 861

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   FVNVEKKS 9
           FVN+ KKS
Sbjct: 634 FVNINKKS 641
>NP_060516
          Length = 714

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NFVNVEKKS 9
           NF N++KK+
Sbjct: 312 NFANMKKKA 320
>NP_060393
          Length = 272

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  NFVNVEKKS 9
          NF+N+ KKS
Sbjct: 9  NFLNMWKKS 17
>NP_839955
          Length = 879

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   FVNVEK 7
           FVNVEK
Sbjct: 695 FVNVEK 700
>NP_653184
          Length = 381

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NFVNVEKKS 9
           N  N+EKKS
Sbjct: 148 NLENLEKKS 156
>NP_001034451
          Length = 427

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NFVNVEKKS 9
           N  N+EKKS
Sbjct: 148 NLENLEKKS 156
>NP_001020095
          Length = 894

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   FVNVEK 7
           FVNVEK
Sbjct: 710 FVNVEK 715
>XP_005265807
          Length = 879

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   FVNVEK 7
           FVNVEK
Sbjct: 695 FVNVEK 700
>XP_006714105
          Length = 897

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   FVNVEK 7
           FVNVEK
Sbjct: 713 FVNVEK 718
>XP_005265805
          Length = 912

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   FVNVEK 7
           FVNVEK
Sbjct: 728 FVNVEK 733
>XP_005265804
          Length = 912

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   FVNVEK 7
           FVNVEK
Sbjct: 728 FVNVEK 733
>NP_060731
          Length = 894

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   FVNVEK 7
           FVNVEK
Sbjct: 710 FVNVEK 715
>NP_852478
          Length = 1179

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NFVNVEKKS 9
           N VN++KK+
Sbjct: 679 NKVNIQKKN 687
>NP_001949
          Length = 463

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NVEKKS 9
           NVEKKS
Sbjct: 440 NVEKKS 445
>NP_004789
          Length = 747

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NFVNVEKKS 9
           NF+ +E+KS
Sbjct: 589 NFIPLEEKS 597
>NP_004791
          Length = 663

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  NFVNVEKKS 9
          NF + EKKS
Sbjct: 45 NFCDEEKKS 53
>NP_005774
          Length = 226

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NFVNVEKKS 9
           NF  +EKK+
Sbjct: 205 NFTKLEKKT 213
>NP_001243556
          Length = 736

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   NFVNVEKK 8
           NF+++EK+
Sbjct: 524 NFISLEKE 531
>NP_003948
          Length = 668

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   NFVNVEKK 8
           NF+++EK+
Sbjct: 524 NFISLEKE 531
>NP_001243559
          Length = 766

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   NFVNVEKK 8
           NF+++EK+
Sbjct: 570 NFISLEKE 577
>NP_001243558
          Length = 674

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   NFVNVEKK 8
           NF+++EK+
Sbjct: 524 NFISLEKE 531
>NP_003595
          Length = 1257

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NFVNVE 6
           N+VNVE
Sbjct: 958 NYVNVE 963
>NP_001269147
          Length = 614

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   NFVNVEKK 8
           NF+++EK+
Sbjct: 464 NFISLEKE 471
>NP_001093143
          Length = 100

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  FVNVEKK 8
          FVNV+K+
Sbjct: 54 FVNVQKE 60
>NP_001093142
          Length = 100

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  FVNVEKK 8
          FVNV+K+
Sbjct: 54 FVNVQKE 60
>NP_001093141
          Length = 100

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  FVNVEKK 8
          FVNV+K+
Sbjct: 54 FVNVQKE 60
>NP_001093140
          Length = 100

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  FVNVEKK 8
          FVNV+K+
Sbjct: 54 FVNVQKE 60
>XP_005262277
          Length = 1256

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NFVNVE 6
           N+VNVE
Sbjct: 958 NYVNVE 963
>XP_006724776
          Length = 1256

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NFVNVE 6
           N+VNVE
Sbjct: 958 NYVNVE 963
>XP_005253277
          Length = 453

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   NFVNVEKK 8
           NF+++EK+
Sbjct: 241 NFISLEKE 248
>XP_006718442
          Length = 675

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   NFVNVEKK 8
           NF+++EK+
Sbjct: 463 NFISLEKE 470
>XP_006718441
          Length = 690

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   NFVNVEKK 8
           NF+++EK+
Sbjct: 546 NFISLEKE 553
>XP_006718440
          Length = 698

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   NFVNVEKK 8
           NF+++EK+
Sbjct: 486 NFISLEKE 493
>XP_006718439
          Length = 698

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   NFVNVEKK 8
           NF+++EK+
Sbjct: 486 NFISLEKE 493
>XP_005253275
          Length = 720

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   NFVNVEKK 8
           NF+++EK+
Sbjct: 524 NFISLEKE 531
>XP_006718438
          Length = 742

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   NFVNVEKK 8
           NF+++EK+
Sbjct: 546 NFISLEKE 553
>XP_006718437
          Length = 758

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   NFVNVEKK 8
           NF+++EK+
Sbjct: 546 NFISLEKE 553
>NP_060499
          Length = 272

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NFVNVEKKS 9
           NF+ +EK S
Sbjct: 173 NFIALEKSS 181
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.304    0.121    0.303 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 530,457
Number of extensions: 631
Number of successful extensions: 41
Number of sequences better than 10000.0: 41
Number of HSP's gapped: 41
Number of HSP's successfully gapped: 41
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)

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