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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              20     390
NP_001075109                                                           20     390
NP_078808                                                              15    9605
NP_001020607                                                           15    9605
NP_060264                                                              15    9605
NP_000383                                                              15    9605
NP_690043                                                              15    9605
NP_000780                                                              15    9605
NP_001171528                                                           15    9605
NP_001177901                                                           15    9605
XP_006721800                                                           15    9605
XP_005257167                                                           15    9605
XP_006717693                                                           15    9605
XP_005251551                                                           15    9605
XP_006716857                                                           15    9605
XP_006712810                                                           15    9605
XP_006712809                                                           15    9605
XP_006712808                                                           15    9605
NP_114171                                                              15    9605

>NP_008835
          Length = 4128

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    NFLLKMTSM 9
            NFLL+MTSM
Sbjct: 2560 NFLLEMTSM 2568



>NP_001075109
          Length = 4097

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    NFLLKMTSM 9
            NFLL+MTSM
Sbjct: 2560 NFLLEMTSM 2568



>NP_078808
          Length = 631

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NFLLKM 6
           NFL+KM
Sbjct: 115 NFLIKM 120



>NP_001020607
          Length = 183

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NFLLKM 6
           NFL+KM
Sbjct: 115 NFLIKM 120



>NP_060264
          Length = 1801

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NFLLKM 6
           NF+LKM
Sbjct: 815 NFILKM 820



>NP_000383
          Length = 1545

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    NFLLKMTS 8
            N+L++MTS
Sbjct: 1253 NWLVRMTS 1260



>NP_690043
          Length = 732

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NFLLKM 6
           NFL+KM
Sbjct: 476 NFLMKM 481



>NP_000780
          Length = 1306

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    NFLLKM 6
            NFL+KM
Sbjct: 1050 NFLMKM 1055



>NP_001171528
          Length = 691

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NFLLKM 6
           NFL+KM
Sbjct: 476 NFLMKM 481



>NP_001177901
          Length = 117

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1  NFLLKMTSM 9
          +FLLK++S+
Sbjct: 82 DFLLKLSSV 90



>XP_006721800
          Length = 616

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NFLLKM 6
           NFL+KM
Sbjct: 360 NFLMKM 365



>XP_005257167
          Length = 1123

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NFLLKM 6
           NFL+KM
Sbjct: 867 NFLMKM 872



>XP_006717693
          Length = 1313

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    NFLLKMTS 8
            N+L++MTS
Sbjct: 1021 NWLVRMTS 1028



>XP_005251551
          Length = 1801

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NFLLKM 6
           NF+LKM
Sbjct: 815 NFILKM 820



>XP_006716857
          Length = 1834

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NFLLKM 6
           NF+LKM
Sbjct: 848 NFILKM 853



>XP_006712810
          Length = 321

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NFLLKM 6
           NFL+KM
Sbjct: 115 NFLIKM 120



>XP_006712809
          Length = 325

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NFLLKM 6
           NFL+KM
Sbjct: 115 NFLIKM 120



>XP_006712808
          Length = 347

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NFLLKM 6
           NFL+KM
Sbjct: 115 NFLIKM 120



>NP_114171
          Length = 798

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NFLLKMTSM 9
           N LLK TS+
Sbjct: 654 NVLLKKTSL 662



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.334    0.133    0.347 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 346,774
Number of extensions: 453
Number of successful extensions: 19
Number of sequences better than 10000.0: 19
Number of HSP's gapped: 19
Number of HSP's successfully gapped: 19
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)