Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001253974                                                           17    3301
NP_005780                                                              17    3301
NP_789839                                                              17    3301
NP_848592                                                              17    3301
XP_005252716                                                           17    3301
XP_005252715                                                           17    3301
XP_006717610                                                           17    3301
NP_006417                                                              17    3301
NP_005219                                                              17    4312
NP_612453                                                              17    4312
NP_056003                                                              17    4312
XP_006717795                                                           17    4312
NP_001121363                                                           16    5631
NP_004521                                                              16    5631
NP_003555                                                              16    5631
NP_001026885                                                           16    5631
NP_001264152                                                           16    5631
NP_001264153                                                           16    5631
NP_060538                                                              16    5631
NP_001129193                                                           16    5631
NP_001271278                                                           16    5631
XP_005273608                                                           16    5631
XP_005273607                                                           16    5631
NP_000104                                                              16    7355
NP_004111                                                              16    7355
NP_078878                                                              16    7355
NP_001269973                                                           16    7355
NP_940890                                                              16    7355
NP_940862                                                              16    7355
NP_055805                                                              16    7355
NP_001269972                                                           16    7355
NP_071442                                                              16    7355
NP_919277                                                              16    7355
XP_006725153                                                           16    7355
XP_006725152                                                           16    7355
XP_006725151                                                           16    7355
XP_006725150                                                           16    7355
XP_005267555                                                           16    7355
XP_006720166                                                           16    7355
XP_005267550                                                           16    7355
XP_005267549                                                           16    7355
XP_005267548                                                           16    7355
XP_006720165                                                           16    7355
XP_006720164                                                           16    7355
XP_006720163                                                           16    7355
XP_006720162                                                           16    7355
XP_006720161                                                           16    7355
XP_005250947                                                           16    7355
XP_005250946                                                           16    7355
XP_005250945                                                           16    7355
XP_005250944                                                           16    7355
XP_005270606                                                           16    7355
XP_006710456                                                           16    7355
NP_001001891                                                           15    9605
NP_110440                                                              15    9605
NP_001245323                                                           15    9605
NP_001245322                                                           15    9605
NP_001245321                                                           15    9605
XP_005274377                                                           15    9605
XP_005274129                                                           15    9605
XP_006718655                                                           15    9605
XP_005274128                                                           15    9605
XP_005274127                                                           15    9605
XP_006712621                                                           15    9605
NP_060327                                                              15    9605
>NP_001253974
          Length = 265

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  NFFPKR 6
          NFFPK+
Sbjct: 43 NFFPKK 48
>NP_005780
          Length = 254

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  NFFPKR 6
          NFFPK+
Sbjct: 32 NFFPKK 37
>NP_789839
          Length = 267

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  NFFPKR 6
          NFFPK+
Sbjct: 32 NFFPKK 37
>NP_848592
          Length = 130

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  NFFPKRARM 9
          +FFP+R R+
Sbjct: 73 HFFPRRPRI 81
>XP_005252716
          Length = 509

 Score = 16.9 bits (32), Expect =  3301
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   NFFPKRARM 9
           NF+P++ R+
Sbjct: 330 NFYPRKGRV 338
>XP_005252715
          Length = 509

 Score = 16.9 bits (32), Expect =  3301
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   NFFPKRARM 9
           NF+P++ R+
Sbjct: 330 NFYPRKGRV 338
>XP_006717610
          Length = 512

 Score = 16.9 bits (32), Expect =  3301
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   NFFPKRARM 9
           NF+P++ R+
Sbjct: 393 NFYPRKGRV 401
>NP_006417
          Length = 572

 Score = 16.9 bits (32), Expect =  3301
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   NFFPKRARM 9
           NF+P++ R+
Sbjct: 393 NFYPRKGRV 401
>NP_005219
          Length = 1210

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    NFFPKRAR 8
            +FFPK A+
Sbjct: 1175 DFFPKEAK 1182
>NP_612453
          Length = 325

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   NFFPKRARM 9
           ++FP+R+R+
Sbjct: 105 DYFPERSRL 113
>NP_056003
          Length = 775

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   NFFPKRAR 8
           N FPKR +
Sbjct: 325 NLFPKRTK 332
>XP_006717795
          Length = 841

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   NFFPKRAR 8
           N FPKR +
Sbjct: 418 NLFPKRTK 425
>NP_001121363
          Length = 610

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1  NFFPKRAR 8
          NFFP++ +
Sbjct: 61 NFFPRKPK 68
>NP_004521
          Length = 660

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   NFFPKRAR 8
           NFFP++ +
Sbjct: 111 NFFPRKPK 118
>NP_003555
          Length = 199

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   NFFPKRAR 8
           N+FPK+++
Sbjct: 149 NWFPKKSK 156
>NP_001026885
          Length = 1027

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   FFPKRAR 8
           FFPK+ R
Sbjct: 230 FFPKKQR 236
>NP_001264152
          Length = 199

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   NFFPKRAR 8
           N+FPK+++
Sbjct: 149 NWFPKKSK 156
>NP_001264153
          Length = 220

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   NFFPKRAR 8
           N+FPK+++
Sbjct: 170 NWFPKKSK 177
>NP_060538
          Length = 973

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   FFPKRA 7
           F+PKRA
Sbjct: 609 FYPKRA 614
>NP_001129193
          Length = 973

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   FFPKRA 7
           F+PKRA
Sbjct: 609 FYPKRA 614
>NP_001271278
          Length = 305

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  FFPKRAR 8
          FFPK+ R
Sbjct: 57 FFPKKQR 63
>XP_005273608
          Length = 804

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   FFPKRA 7
           F+PKRA
Sbjct: 609 FYPKRA 614
>XP_005273607
          Length = 937

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   FFPKRA 7
           F+PKRA
Sbjct: 609 FYPKRA 614
>NP_000104
          Length = 332

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   FFPKRARM 9
           FFPK  R+
Sbjct: 306 FFPKEERV 313
>NP_004111
          Length = 591

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2   FFPKR 6
           FFPKR
Sbjct: 227 FFPKR 231
>NP_078878
          Length = 861

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   NFFPKR 6
           NF PKR
Sbjct: 304 NFMPKR 309
>NP_001269973
          Length = 2083

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    NFFPKRAR 8
            NF+ +RAR
Sbjct: 2039 NFYEERAR 2046
>NP_940890
          Length = 1179

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   FPKRARM 9
           FPKRA +
Sbjct: 322 FPKRAHL 328
>NP_940862
          Length = 633

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2   FFPKR 6
           FFPKR
Sbjct: 227 FFPKR 231
>NP_055805
          Length = 2036

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    NFFPKRAR 8
            NF+ +RAR
Sbjct: 1992 NFYEERAR 1999
>NP_001269972
          Length = 2049

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    NFFPKRAR 8
            NF+ +RAR
Sbjct: 2005 NFYEERAR 2012
>NP_071442
          Length = 690

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   NFFPKR 6
           N FPKR
Sbjct: 280 NIFPKR 285
>NP_919277
          Length = 2083

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    NFFPKRAR 8
            NF+ +RAR
Sbjct: 1992 NFYEERAR 1999
>XP_006725153
          Length = 1179

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   FPKRARM 9
           FPKRA +
Sbjct: 322 FPKRAHL 328
>XP_006725152
          Length = 1179

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   FPKRARM 9
           FPKRA +
Sbjct: 322 FPKRAHL 328
>XP_006725151
          Length = 1185

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   FPKRARM 9
           FPKRA +
Sbjct: 328 FPKRAHL 334
>XP_006725150
          Length = 1198

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   FPKRARM 9
           FPKRA +
Sbjct: 341 FPKRAHL 347
>XP_005267555
          Length = 1325

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    NFFPKRAR 8
            NF+ +RAR
Sbjct: 1233 NFYEERAR 1240
>XP_006720166
          Length = 2083

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    NFFPKRAR 8
            NF+ +RAR
Sbjct: 2039 NFYEERAR 2046
>XP_005267550
          Length = 2084

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    NFFPKRAR 8
            NF+ +RAR
Sbjct: 1992 NFYEERAR 1999
>XP_005267549
          Length = 2130

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    NFFPKRAR 8
            NF+ +RAR
Sbjct: 2039 NFYEERAR 2046
>XP_005267548
          Length = 2131

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    NFFPKRAR 8
            NF+ +RAR
Sbjct: 2039 NFYEERAR 2046
>XP_006720165
          Length = 2542

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    NFFPKRAR 8
            NF+ +RAR
Sbjct: 2498 NFYEERAR 2505
>XP_006720164
          Length = 2542

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    NFFPKRAR 8
            NF+ +RAR
Sbjct: 2498 NFYEERAR 2505
>XP_006720163
          Length = 2543

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    NFFPKRAR 8
            NF+ +RAR
Sbjct: 2451 NFYEERAR 2458
>XP_006720162
          Length = 2589

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    NFFPKRAR 8
            NF+ +RAR
Sbjct: 2498 NFYEERAR 2505
>XP_006720161
          Length = 2590

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    NFFPKRAR 8
            NF+ +RAR
Sbjct: 2498 NFYEERAR 2505
>XP_005250947
          Length = 1179

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   FPKRARM 9
           FPKRA +
Sbjct: 322 FPKRAHL 328
>XP_005250946
          Length = 1179

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   FPKRARM 9
           FPKRA +
Sbjct: 322 FPKRAHL 328
>XP_005250945
          Length = 1185

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   FPKRARM 9
           FPKRA +
Sbjct: 328 FPKRAHL 334
>XP_005250944
          Length = 1198

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   FPKRARM 9
           FPKRA +
Sbjct: 341 FPKRAHL 347
>XP_005270606
          Length = 503

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2   FFPKR 6
           FFPKR
Sbjct: 97  FFPKR 101
>XP_006710456
          Length = 633

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2   FFPKR 6
           FFPKR
Sbjct: 227 FFPKR 231
>NP_001001891
          Length = 933

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   FFPKRAR 8
           +FP+R+R
Sbjct: 487 YFPERSR 493
>NP_110440
          Length = 707

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NFFPKRAR 8
           NF+ KRA+
Sbjct: 585 NFWAKRAK 592
>NP_001245323
          Length = 662

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NFFPKRAR 8
           NF+ KRA+
Sbjct: 540 NFWAKRAK 547
>NP_001245322
          Length = 648

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NFFPKRAR 8
           NF+ KRA+
Sbjct: 526 NFWAKRAK 533
>NP_001245321
          Length = 677

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NFFPKRAR 8
           NF+ KRA+
Sbjct: 555 NFWAKRAK 562
>XP_005274377
          Length = 678

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NFFPKRAR 8
           NF+ KRA+
Sbjct: 556 NFWAKRAK 563
>XP_005274129
          Length = 568

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   NFFPKR 6
           NF+P+R
Sbjct: 276 NFYPER 281
>XP_006718655
          Length = 596

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   NFFPKR 6
           NF+P+R
Sbjct: 304 NFYPER 309
>XP_005274128
          Length = 605

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   NFFPKR 6
           NF+P+R
Sbjct: 313 NFYPER 318
>XP_005274127
          Length = 668

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   NFFPKR 6
           NF+P+R
Sbjct: 376 NFYPER 381
>XP_006712621
          Length = 932

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   FFPKRAR 8
           +FP+R+R
Sbjct: 486 YFPERSR 492
>NP_060327
          Length = 659

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   NFFPKR 6
           NF+P+R
Sbjct: 367 NFYPER 372
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.344    0.146    0.482 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 623,278
Number of extensions: 823
Number of successful extensions: 65
Number of sequences better than 10000.0: 65
Number of HSP's gapped: 65
Number of HSP's successfully gapped: 65
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.7 bits)
S2: 28 (15.4 bits)

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