Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

XP_006721057                                                           19     869
NP_001020015                                                           18    1482
NP_002222                                                              18    1482
NP_000042                                                              18    1482
XP_005271621                                                           18    1482
XP_006718907                                                           18    1482
XP_006718906                                                           18    1482
XP_005271619                                                           18    1482
XP_005271618                                                           18    1482
XP_006718286                                                           18    1482
XP_006718285                                                           18    1482
NP_001080                                                              17    3301
NP_061198                                                              17    4312
XP_006720670                                                           17    4312
NP_001139320                                                           16    5631
NP_001139321                                                           16    5631
NP_001139323                                                           16    5631
NP_006008                                                              16    5631
NP_001139322                                                           16    5631
NP_001139324                                                           16    5631
NP_001139319                                                           16    5631
NP_001765                                                              16    5631
NP_976222                                                              16    5631
XP_005259492                                                           16    5631
XP_006714037                                                           16    5631
XP_005248253                                                           16    5631
XP_005248252                                                           16    5631
XP_006714036                                                           16    5631
NP_001122306                                                           16    7355
NP_000524                                                              16    7355
NP_955772                                                              16    7355
NP_001984                                                              16    7355
NP_848657                                                              16    7355
NP_001191140                                                           16    7355
NP_001191139                                                           16    7355
NP_149116                                                              16    7355
XP_006716023                                                           16    7355
XP_005267241                                                           16    7355
NP_055064                                                              15    9605
NP_057430                                                              15    9605
NP_036471                                                              15    9605
NP_060415                                                              15    9605
NP_078904                                                              15    9605
NP_001154814                                                           15    9605
NP_001182412                                                           15    9605
NP_063939                                                              15    9605
NP_775761                                                              15    9605
XP_005259084                                                           15    9605
XP_006723316                                                           15    9605
XP_005273481                                                           15    9605
XP_005248489                                                           15    9605
XP_005248488                                                           15    9605
NP_001027006                                                           15    9605
NP_060324                                                              15    9605
>XP_006721057
          Length = 257

 Score = 18.9 bits (37), Expect =   869
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   NFFLLGAII 9
           NF LLGA+I
Sbjct: 208 NFLLLGAVI 216
>NP_001020015
          Length = 242

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  FFLLGAII 9
          FF+LGA+I
Sbjct: 20 FFILGAVI 27
>NP_002222
          Length = 267

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  FFLLGAII 9
          FF+LGA+I
Sbjct: 20 FFILGAVI 27
>NP_000042
          Length = 3056

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NFFLLGAII 9
           NF LLGAII
Sbjct: 504 NFGLLGAII 512
>XP_005271621
          Length = 2708

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NFFLLGAII 9
           NF LLGAII
Sbjct: 156 NFGLLGAII 164
>XP_006718907
          Length = 3050

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NFFLLGAII 9
           NF LLGAII
Sbjct: 504 NFGLLGAII 512
>XP_006718906
          Length = 3056

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NFFLLGAII 9
           NF LLGAII
Sbjct: 504 NFGLLGAII 512
>XP_005271619
          Length = 3056

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NFFLLGAII 9
           NF LLGAII
Sbjct: 504 NFGLLGAII 512
>XP_005271618
          Length = 3056

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NFFLLGAII 9
           NF LLGAII
Sbjct: 504 NFGLLGAII 512
>XP_006718286
          Length = 267

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  FFLLGAII 9
          FF+LGA+I
Sbjct: 20 FFILGAVI 27
>XP_006718285
          Length = 308

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  FFLLGAII 9
          FF+LGA+I
Sbjct: 61 FFILGAVI 68
>NP_001080
          Length = 1704

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    NFFLLGA 7
            NFF LGA
Sbjct: 1200 NFFFLGA 1206
>NP_061198
          Length = 1742

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    FFLLGAI 8
            FFL+GA+
Sbjct: 1580 FFLIGAV 1586
>XP_006720670
          Length = 1712

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    FFLLGAI 8
            FFL+GA+
Sbjct: 1550 FFLIGAV 1556
>NP_001139320
          Length = 856

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NFFLLGA 7
           N FLLGA
Sbjct: 597 NIFLLGA 603
>NP_001139321
          Length = 842

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NFFLLGA 7
           N FLLGA
Sbjct: 606 NIFLLGA 612
>NP_001139323
          Length = 833

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NFFLLGA 7
           N FLLGA
Sbjct: 597 NIFLLGA 603
>NP_006008
          Length = 865

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NFFLLGA 7
           N FLLGA
Sbjct: 606 NIFLLGA 612
>NP_001139322
          Length = 834

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NFFLLGA 7
           N FLLGA
Sbjct: 606 NIFLLGA 612
>NP_001139324
          Length = 825

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NFFLLGA 7
           N FLLGA
Sbjct: 597 NIFLLGA 603
>NP_001139319
          Length = 856

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NFFLLGA 7
           N FLLGA
Sbjct: 597 NIFLLGA 603
>NP_001765
          Length = 281

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  FFLLGAII 9
          FF+LG++I
Sbjct: 22 FFVLGSLI 29
>NP_976222
          Length = 184

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  FLLGAII 9
          FLLGAI+
Sbjct: 4  FLLGAIL 10
>XP_005259492
          Length = 365

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  FFLLGAII 9
          FF+LG++I
Sbjct: 22 FFVLGSLI 29
>XP_006714037
          Length = 787

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NFFLLGA 7
           N FLLGA
Sbjct: 528 NIFLLGA 534
>XP_005248253
          Length = 825

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NFFLLGA 7
           N FLLGA
Sbjct: 597 NIFLLGA 603
>XP_005248252
          Length = 833

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NFFLLGA 7
           N FLLGA
Sbjct: 597 NIFLLGA 603
>XP_006714036
          Length = 856

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NFFLLGA 7
           N FLLGA
Sbjct: 597 NIFLLGA 603
>NP_001122306
          Length = 277

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  FFLLGAII 9
          FFL GA++
Sbjct: 79 FFLYGALL 86
>NP_000524
          Length = 277

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  FFLLGAII 9
          FFL GA++
Sbjct: 79 FFLYGALL 86
>NP_955772
          Length = 242

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  FFLLGAII 9
          FFL GA++
Sbjct: 79 FFLYGALL 86
>NP_001984
          Length = 295

 Score = 15.8 bits (29), Expect =  7355
 Identities = 3/8 (37%), Positives = 8/8 (100%)

Query: 2   FFLLGAII 9
           F+++GA++
Sbjct: 253 FYIIGAVV 260
>NP_848657
          Length = 283

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   NFFLLGAI 8
           N FLLG +
Sbjct: 235 NLFLLGGV 242
>NP_001191140
          Length = 196

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   FFLLGAII 9
           +FLLG+++
Sbjct: 105 YFLLGSVL 112
>NP_001191139
          Length = 223

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   FFLLGAII 9
           +FLLG+++
Sbjct: 132 YFLLGSVL 139
>NP_149116
          Length = 577

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   NFFLLGAI 8
           N FLLG +
Sbjct: 390 NLFLLGVV 397
>XP_006716023
          Length = 282

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   NFFLLGAI 8
           N FLLG +
Sbjct: 234 NLFLLGGV 241
>XP_005267241
          Length = 407

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   NFFLLGAI 8
           N FLLG +
Sbjct: 220 NLFLLGVV 227
>NP_055064
          Length = 410

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   FLLGAI 8
           FLLGAI
Sbjct: 261 FLLGAI 266
>NP_057430
          Length = 367

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   FLLGAI 8
           FLLGAI
Sbjct: 261 FLLGAI 266
>NP_036471
          Length = 294

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2  FFLLGAII 9
          F+L+GA++
Sbjct: 31 FWLIGALV 38
>NP_060415
          Length = 424

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  FLLGAI 8
          FLLGAI
Sbjct: 20 FLLGAI 25
>NP_078904
          Length = 714

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   FLLGAI 8
           FLLGAI
Sbjct: 284 FLLGAI 289
>NP_001154814
          Length = 323

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2  FFLLGAII 9
          FF+ GA++
Sbjct: 25 FFVFGAVL 32
>NP_001182412
          Length = 714

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   FLLGAI 8
           FLLGAI
Sbjct: 284 FLLGAI 289
>NP_063939
          Length = 486

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NFFLLGAI 8
           +FFL GA+
Sbjct: 338 DFFLTGAL 345
>NP_775761
          Length = 324

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2  FFLLGAII 9
          FF+ GA++
Sbjct: 25 FFVFGAVL 32
>XP_005259084
          Length = 99

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  FFLLGAI 8
          FFLLG +
Sbjct: 13 FFLLGGL 19
>XP_006723316
          Length = 120

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  FFLLGAI 8
          FFLLG +
Sbjct: 34 FFLLGGL 40
>XP_005273481
          Length = 726

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   FFLLGAII 9
           FF LG++I
Sbjct: 295 FFTLGSLI 302
>XP_005248489
          Length = 353

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2  FFLLGAII 9
          FF+ GA++
Sbjct: 54 FFVFGAVL 61
>XP_005248488
          Length = 352

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2  FFLLGAII 9
          FF+ GA++
Sbjct: 54 FFVFGAVL 61
>NP_001027006
          Length = 1149

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   FFLLGAII 9
           FF LG++I
Sbjct: 295 FFTLGSLI 302
>NP_060324
          Length = 188

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   FFLLGAI 8
           FFLLG +
Sbjct: 102 FFLLGGL 108
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.350    0.170    0.510 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 421,500
Number of extensions: 604
Number of successful extensions: 54
Number of sequences better than 10000.0: 54
Number of HSP's gapped: 54
Number of HSP's successfully gapped: 54
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.7 bits)
S2: 28 (15.4 bits)

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