Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              20     509
NP_671714                                                              20     509
XP_006715890                                                           20     509
XP_005250160                                                           20     509
XP_005250159                                                           20     509
NP_596870                                                              18    1935
NP_443198                                                              18    1935
NP_570824                                                              17    4312
NP_055907                                                              17    4312
NP_003072                                                              17    4312
XP_005260867                                                           17    4312
XP_005260865                                                           17    4312
XP_005255242                                                           17    4312
XP_005255241                                                           17    4312
XP_005255240                                                           17    4312
XP_005255239                                                           17    4312
NP_002473                                                              16    5631
NP_001182122                                                           16    5631
NP_689511                                                              16    5631
XP_005248250                                                           16    5631
XP_005270946                                                           16    5631
XP_005270945                                                           16    5631
NP_001121798                                                           16    5631
NP_444270                                                              16    5631
NP_001276089                                                           16    7355
NP_005922                                                              16    7355
NP_000238                                                              16    7355
NP_001276081                                                           16    7355
NP_001170990                                                           16    7355
NP_001276082                                                           16    7355
NP_055625                                                              16    7355
XP_006726158                                                           16    7355
XP_006726065                                                           16    7355
XP_006711734                                                           16    7355
XP_006711733                                                           16    7355
XP_006711732                                                           16    7355
XP_006711731                                                           16    7355
XP_006711730                                                           16    7355
XP_006711729                                                           16    7355
XP_005245695                                                           16    7355
NP_067632                                                              16    7355
NP_003752                                                              15    9605
NP_001186217                                                           15    9605
NP_001186216                                                           15    9605
NP_001186215                                                           15    9605
NP_001186214                                                           15    9605
NP_004696                                                              15    9605
NP_001008489                                                           15    9605
NP_057279                                                              15    9605
NP_001271188                                                           15    9605
XP_006714161                                                           15    9605
XP_006713927                                                           15    9605
NP_660334                                                              15    9605
>NP_005742
          Length = 3907

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NEMSQNIET 9
           NEMSQ IET
Sbjct: 672 NEMSQKIET 680
>NP_671714
          Length = 3899

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NEMSQNIET 9
           NEMSQ IET
Sbjct: 672 NEMSQKIET 680
>XP_006715890
          Length = 3860

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NEMSQNIET 9
           NEMSQ IET
Sbjct: 672 NEMSQKIET 680
>XP_005250160
          Length = 3939

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NEMSQNIET 9
           NEMSQ IET
Sbjct: 672 NEMSQKIET 680
>XP_005250159
          Length = 3952

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NEMSQNIET 9
           NEMSQ IET
Sbjct: 672 NEMSQKIET 680
>NP_596870
          Length = 5604

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    NEMSQNIET 9
            N+M QNIE+
Sbjct: 3778 NQMDQNIES 3786
>NP_443198
          Length = 928

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NEMSQNIET 9
           NE+SQN +T
Sbjct: 374 NEVSQNFQT 382
>NP_570824
          Length = 206

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NEMSQNIE 8
           NEM +N+E
Sbjct: 144 NEMDENLE 151
>NP_055907
          Length = 3661

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    MSQNIET 9
            M+QNIET
Sbjct: 2375 MTQNIET 2381
>NP_003072
          Length = 206

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NEMSQNIE 8
           NEM +N+E
Sbjct: 144 NEMDENLE 151
>XP_005260867
          Length = 206

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NEMSQNIE 8
           NEM +N+E
Sbjct: 144 NEMDENLE 151
>XP_005260865
          Length = 206

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NEMSQNIE 8
           NEM +N+E
Sbjct: 144 NEMDENLE 151
>XP_005255242
          Length = 3609

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    MSQNIET 9
            M+QNIET
Sbjct: 2323 MTQNIET 2329
>XP_005255241
          Length = 3637

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    MSQNIET 9
            M+QNIET
Sbjct: 2351 MTQNIET 2357
>XP_005255240
          Length = 3663

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    MSQNIET 9
            M+QNIET
Sbjct: 2377 MTQNIET 2383
>XP_005255239
          Length = 3689

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    MSQNIET 9
            M+QNIET
Sbjct: 2403 MTQNIET 2409
>NP_002473
          Length = 788

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NEMSQNIE 8
           NE+S+N+E
Sbjct: 753 NEVSENME 760
>NP_001182122
          Length = 724

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NEMSQNIE 8
           NE+S+N+E
Sbjct: 689 NEVSENME 696
>NP_689511
          Length = 449

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NEMSQNIE 8
           NE+S+N+E
Sbjct: 414 NEVSENME 421
>XP_005248250
          Length = 1074

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   EMSQNIET 9
           +MS NIET
Sbjct: 834 DMSPNIET 841
>XP_005270946
          Length = 451

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NEMSQNIE 8
           NE+S+N+E
Sbjct: 416 NEVSENME 423
>XP_005270945
          Length = 790

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NEMSQNIE 8
           NE+S+N+E
Sbjct: 755 NEVSENME 762
>NP_001121798
          Length = 132

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  NEMSQNIET 9
          NE+S  +ET
Sbjct: 25 NELSSTVET 33
>NP_444270
          Length = 1074

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   EMSQNIET 9
           +MS NIET
Sbjct: 834 DMSPNIET 841
>NP_001276089
          Length = 351

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MSQNIET 9
           +SQN+ET
Sbjct: 109 LSQNLET 115
>NP_005922
          Length = 383

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MSQNIET 9
           +SQN+ET
Sbjct: 141 LSQNLET 147
>NP_000238
          Length = 383

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MSQNIET 9
           +SQN+ET
Sbjct: 141 LSQNLET 147
>NP_001276081
          Length = 235

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  MSQNIET 9
          +SQN+ET
Sbjct: 44 LSQNLET 50
>NP_001170990
          Length = 332

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MSQNIET 9
           +SQN+ET
Sbjct: 141 LSQNLET 147
>NP_001276082
          Length = 235

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  MSQNIET 9
          +SQN+ET
Sbjct: 44 LSQNLET 50
>NP_055625
          Length = 3117

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    EMSQNIET 9
            E+SQ++ET
Sbjct: 2107 ELSQDLET 2114
>XP_006726158
          Length = 351

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MSQNIET 9
           +SQN+ET
Sbjct: 109 LSQNLET 115
>XP_006726065
          Length = 351

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MSQNIET 9
           +SQN+ET
Sbjct: 109 LSQNLET 115
>XP_006711734
          Length = 3136

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    EMSQNIET 9
            E+SQ++ET
Sbjct: 2126 ELSQDLET 2133
>XP_006711733
          Length = 3137

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    EMSQNIET 9
            E+SQ++ET
Sbjct: 2127 ELSQDLET 2134
>XP_006711732
          Length = 3154

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    EMSQNIET 9
            E+SQ++ET
Sbjct: 2144 ELSQDLET 2151
>XP_006711731
          Length = 3157

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    EMSQNIET 9
            E+SQ++ET
Sbjct: 2147 ELSQDLET 2154
>XP_006711730
          Length = 3157

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    EMSQNIET 9
            E+SQ++ET
Sbjct: 2147 ELSQDLET 2154
>XP_006711729
          Length = 3158

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    EMSQNIET 9
            E+SQ++ET
Sbjct: 2148 ELSQDLET 2155
>XP_005245695
          Length = 3158

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    EMSQNIET 9
            E+SQ++ET
Sbjct: 2148 ELSQDLET 2155
>NP_067632
          Length = 367

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   NEMSQNIE 8
           NE  QN+E
Sbjct: 169 NEQEQNLE 176
>NP_003752
          Length = 100

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  NEMSQNIE 8
          N M+QN+E
Sbjct: 25 NIMTQNVE 32
>NP_001186217
          Length = 241

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NEMSQNIE 8
           + MSQN+E
Sbjct: 209 SRMSQNLE 216
>NP_001186216
          Length = 241

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NEMSQNIE 8
           + MSQN+E
Sbjct: 209 SRMSQNLE 216
>NP_001186215
          Length = 241

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NEMSQNIE 8
           + MSQN+E
Sbjct: 209 SRMSQNLE 216
>NP_001186214
          Length = 241

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NEMSQNIE 8
           + MSQN+E
Sbjct: 209 SRMSQNLE 216
>NP_004696
          Length = 761

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NEMSQNIE 8
           NE+ +NIE
Sbjct: 522 NEVMENIE 529
>NP_001008489
          Length = 241

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NEMSQNIE 8
           + MSQN+E
Sbjct: 209 SRMSQNLE 216
>NP_057279
          Length = 1780

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   EMSQNIET 9
           E +QN+ET
Sbjct: 543 EETQNVET 550
>NP_001271188
          Length = 1820

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   EMSQNIET 9
           E +QN+ET
Sbjct: 543 EETQNVET 550
>XP_006714161
          Length = 1020

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  NEMSQNI 7
          NE++QNI
Sbjct: 5  NEINQNI 11
>XP_006713927
          Length = 765

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   EMSQNIET 9
           E+SQ IET
Sbjct: 598 ELSQAIET 605
>NP_660334
          Length = 765

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   EMSQNIET 9
           E+SQ IET
Sbjct: 598 ELSQAIET 605
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.287    0.104    0.241 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 357,788
Number of extensions: 634
Number of successful extensions: 53
Number of sequences better than 10000.0: 53
Number of HSP's gapped: 53
Number of HSP's successfully gapped: 53
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.9 bits)
S2: 28 (15.4 bits)

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