Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004251                                                              20     299
NP_001177370                                                           18    1482
NP_001258218                                                           17    3301
NP_001258217                                                           17    3301
NP_001258216                                                           17    3301
NP_001258247                                                           17    3301
NP_115810                                                              17    3301
NP_001258215                                                           17    3301
XP_005270370                                                           17    3301
XP_006717680                                                           17    3301
NP_694579                                                              17    3301
NP_002299                                                              16    5631
NP_033665                                                              16    5631
NP_694536                                                              16    5631
NP_003788                                                              16    5631
NP_004805                                                              16    5631
NP_001013725                                                           16    5631
NP_001361                                                              16    5631
NP_001157968                                                           16    5631
XP_006721955                                                           16    5631
XP_005254450                                                           16    5631
XP_005254449                                                           16    5631
XP_005274430                                                           16    5631
XP_006718794                                                           16    5631
XP_006718793                                                           16    5631
XP_005247674                                                           16    5631
XP_006713761                                                           16    5631
XP_006713760                                                           16    5631
XP_006713759                                                           16    5631
XP_006712019                                                           16    5631
NP_619542                                                              16    7355
NP_001265519                                                           16    7355
NP_006850                                                              16    7355
NP_739580                                                              16    7355
NP_055552                                                              16    7355
NP_114147                                                              16    7355
NP_775901                                                              16    7355
NP_001257609                                                           16    7355
NP_001257608                                                           16    7355
NP_612366                                                              16    7355
XP_005274600                                                           16    7355
XP_006723732                                                           16    7355
XP_006723731                                                           16    7355
XP_005260952                                                           16    7355
XP_005260150                                                           16    7355
XP_006722498                                                           16    7355
XP_006722497                                                           16    7355
XP_006714342                                                           16    7355
XP_005263203                                                           16    7355
XP_005263202                                                           16    7355
XP_005263201                                                           16    7355
XP_005263200                                                           16    7355
XP_006714053                                                           16    7355
NP_064611                                                              16    7355
NP_001092873                                                           16    7355
NP_115642                                                              15    9605
NP_776173                                                              15    9605
XP_005256370                                                           15    9605
XP_006719419                                                           15    9605
XP_006719417                                                           15    9605
XP_006719416                                                           15    9605
XP_006719415                                                           15    9605
XP_006719414                                                           15    9605
XP_006719413                                                           15    9605
XP_006719412                                                           15    9605
XP_006719411                                                           15    9605
NP_057293                                                              15    9605
>NP_004251
          Length = 1208

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    NELAFHLRS 9
            +ELAFHLRS
Sbjct: 1031 SELAFHLRS 1039
>NP_001177370
          Length = 142

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  NELAFHLR 8
          +E+AFHLR
Sbjct: 48 SEIAFHLR 55
>NP_001258218
          Length = 462

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LAFHLRS 9
           LA+HLRS
Sbjct: 197 LAYHLRS 203
>NP_001258217
          Length = 490

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LAFHLRS 9
           LA+HLRS
Sbjct: 225 LAYHLRS 231
>NP_001258216
          Length = 490

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LAFHLRS 9
           LA+HLRS
Sbjct: 225 LAYHLRS 231
>NP_001258247
          Length = 490

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LAFHLRS 9
           LA+HLRS
Sbjct: 225 LAYHLRS 231
>NP_115810
          Length = 567

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LAFHLRS 9
           LA+HLRS
Sbjct: 302 LAYHLRS 308
>NP_001258215
          Length = 538

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LAFHLRS 9
           LA+HLRS
Sbjct: 273 LAYHLRS 279
>XP_005270370
          Length = 340

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NELAFHLRS 9
           ++L FH+RS
Sbjct: 288 SDLVFHMRS 296
>XP_006717680
          Length = 340

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NELAFHLRS 9
           ++L FH+RS
Sbjct: 288 SDLVFHMRS 296
>NP_694579
          Length = 340

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NELAFHLRS 9
           ++L FH+RS
Sbjct: 288 SDLVFHMRS 296
>NP_002299
          Length = 323

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   NELAFHL 7
           N +AFHL
Sbjct: 230 NHIAFHL 236
>NP_033665
          Length = 355

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   NELAFHL 7
           N +AFHL
Sbjct: 262 NHIAFHL 268
>NP_694536
          Length = 400

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   NELAFHLR 8
           NEL FH R
Sbjct: 194 NELKFHPR 201
>NP_003788
          Length = 441

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   NELAFHLR 8
           NEL FH R
Sbjct: 194 NELKFHPR 201
>NP_004805
          Length = 357

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NELAFH 6
           NE+AFH
Sbjct: 331 NEVAFH 336
>NP_001013725
          Length = 1649

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   ELAFHLRS 9
           ELA+H++S
Sbjct: 125 ELAYHVQS 132
>NP_001361
          Length = 4158

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   NELAFHL 7
           NEL +HL
Sbjct: 320 NELCYHL 326
>NP_001157968
          Length = 1854

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    ELAFHLR 8
            ELA HLR
Sbjct: 1550 ELALHLR 1556
>XP_006721955
          Length = 311

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   NELAFHL 7
           N +AFHL
Sbjct: 218 NHIAFHL 224
>XP_005254450
          Length = 1505

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   ELAFHLRS 9
           ELA+H++S
Sbjct: 125 ELAYHVQS 132
>XP_005254449
          Length = 1626

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   ELAFHLRS 9
           ELA+H++S
Sbjct: 125 ELAYHVQS 132
>XP_005274430
          Length = 361

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   NELAFHLR 8
           NEL FH R
Sbjct: 194 NELKFHPR 201
>XP_006718794
          Length = 390

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   NELAFHLR 8
           NEL FH R
Sbjct: 194 NELKFHPR 201
>XP_006718793
          Length = 403

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   NELAFHLR 8
           NEL FH R
Sbjct: 194 NELKFHPR 201
>XP_005247674
          Length = 1089

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   ELAFHLR 8
           ELA HLR
Sbjct: 785 ELALHLR 791
>XP_006713761
          Length = 1554

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    ELAFHLR 8
            ELA HLR
Sbjct: 1250 ELALHLR 1256
>XP_006713760
          Length = 1847

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    ELAFHLR 8
            ELA HLR
Sbjct: 1543 ELALHLR 1549
>XP_006713759
          Length = 1898

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    ELAFHLR 8
            ELA HLR
Sbjct: 1594 ELALHLR 1600
>XP_006712019
          Length = 4158

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   NELAFHL 7
           NEL +HL
Sbjct: 320 NELCYHL 326
>NP_619542
          Length = 1041

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   NELAFHL 7
           NEL +HL
Sbjct: 899 NELRYHL 905
>NP_001265519
          Length = 329

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   NELAFH 6
           NEL FH
Sbjct: 293 NELGFH 298
>NP_006850
          Length = 372

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   NELAFH 6
           NEL FH
Sbjct: 336 NELGFH 341
>NP_739580
          Length = 326

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  NELAFHLRS 9
          NEL  HL++
Sbjct: 21 NELLLHLKT 29
>NP_055552
          Length = 326

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  NELAFHLRS 9
          NEL  HL++
Sbjct: 21 NELLLHLKT 29
>NP_114147
          Length = 703

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ELAFHLRS 9
           E+AF LRS
Sbjct: 420 EVAFQLRS 427
>NP_775901
          Length = 2226

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   LAFHLRS 9
           LAFHL S
Sbjct: 779 LAFHLTS 785
>NP_001257609
          Length = 302

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   NELAFHLR 8
           N+L +H+R
Sbjct: 105 NDLTYHVR 112
>NP_001257608
          Length = 330

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   NELAFHLR 8
           N+L +H+R
Sbjct: 154 NDLTYHVR 161
>NP_612366
          Length = 351

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   NELAFHLR 8
           N+L +H+R
Sbjct: 154 NDLTYHVR 161
>XP_005274600
          Length = 1059

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   NELAFHL 7
           NEL +HL
Sbjct: 917 NELRYHL 923
>XP_006723732
          Length = 326

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  NELAFHLRS 9
          NEL  HL++
Sbjct: 21 NELLLHLKT 29
>XP_006723731
          Length = 326

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  NELAFHLRS 9
          NEL  HL++
Sbjct: 21 NELLLHLKT 29
>XP_005260952
          Length = 326

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  NELAFHLRS 9
          NEL  HL++
Sbjct: 21 NELLLHLKT 29
>XP_005260150
          Length = 703

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ELAFHLRS 9
           E+AF LRS
Sbjct: 420 EVAFQLRS 427
>XP_006722498
          Length = 2059

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   LAFHLRS 9
           LAFHL S
Sbjct: 780 LAFHLTS 786
>XP_006722497
          Length = 2227

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   LAFHLRS 9
           LAFHL S
Sbjct: 780 LAFHLTS 786
>XP_006714342
          Length = 690

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   NELAFHLRS 9
           ++L +H+RS
Sbjct: 639 SDLVYHMRS 647
>XP_005263203
          Length = 689

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   NELAFHLRS 9
           ++L +H+RS
Sbjct: 638 SDLVYHMRS 646
>XP_005263202
          Length = 690

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   NELAFHLRS 9
           ++L +H+RS
Sbjct: 639 SDLVYHMRS 647
>XP_005263201
          Length = 690

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   NELAFHLRS 9
           ++L +H+RS
Sbjct: 639 SDLVYHMRS 647
>XP_005263200
          Length = 873

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   NELAFHLRS 9
           ++L +H+RS
Sbjct: 822 SDLVYHMRS 830
>XP_006714053
          Length = 226

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   NELAFH 6
           NEL FH
Sbjct: 190 NELGFH 195
>NP_064611
          Length = 689

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   NELAFHLRS 9
           ++L +H+RS
Sbjct: 638 SDLVYHMRS 646
>NP_001092873
          Length = 689

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   NELAFHLRS 9
           ++L +H+RS
Sbjct: 638 SDLVYHMRS 646
>NP_115642
          Length = 1984

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    ELAFHLR 8
            ELA HLR
Sbjct: 1317 ELAAHLR 1323
>NP_776173
          Length = 758

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NELAFH 6
           +ELAFH
Sbjct: 534 SELAFH 539
>XP_005256370
          Length = 164

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1  NELAFH 6
          NEL FH
Sbjct: 49 NELKFH 54
>XP_006719419
          Length = 443

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NELAFH 6
           +ELAFH
Sbjct: 219 SELAFH 224
>XP_006719417
          Length = 597

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NELAFH 6
           +ELAFH
Sbjct: 534 SELAFH 539
>XP_006719416
          Length = 615

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NELAFH 6
           +ELAFH
Sbjct: 391 SELAFH 396
>XP_006719415
          Length = 615

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NELAFH 6
           +ELAFH
Sbjct: 391 SELAFH 396
>XP_006719414
          Length = 708

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NELAFH 6
           +ELAFH
Sbjct: 484 SELAFH 489
>XP_006719413
          Length = 716

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NELAFH 6
           +ELAFH
Sbjct: 534 SELAFH 539
>XP_006719412
          Length = 724

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NELAFH 6
           +ELAFH
Sbjct: 534 SELAFH 539
>XP_006719411
          Length = 738

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NELAFH 6
           +ELAFH
Sbjct: 514 SELAFH 519
>NP_057293
          Length = 140

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1  NELAFH 6
          NEL FH
Sbjct: 24 NELKFH 29
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.327    0.135    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 489,485
Number of extensions: 854
Number of successful extensions: 67
Number of sequences better than 10000.0: 67
Number of HSP's gapped: 67
Number of HSP's successfully gapped: 67
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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