Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001136098                                                           20     390
NP_000360                                                              20     390
NP_001018046                                                           20     390
XP_005268096                                                           20     390
XP_006720308                                                           20     390
XP_005268094                                                           20     390
NP_000224                                                              19     665
NP_006176                                                              17    4312
NP_001273490                                                           17    4312
XP_006718633                                                           17    4312
XP_006718632                                                           17    4312
XP_006718631                                                           17    4312
XP_006718630                                                           17    4312
XP_006718629                                                           17    4312
XP_006718628                                                           17    4312
XP_006718627                                                           17    4312
XP_006718626                                                           17    4312
XP_006718625                                                           17    4312
NP_444267                                                              16    5631
XP_005265754                                                           16    5631
NP_001193933                                                           16    5631
NP_065170                                                              16    7355
NP_001124335                                                           15    9605
NP_006200                                                              15    9605
NP_001035181                                                           15    9605
NP_003130                                                              15    9605
NP_073728                                                              15    9605
XP_006716650                                                           15    9605
XP_006716649                                                           15    9605
XP_006716648                                                           15    9605
XP_006716647                                                           15    9605
XP_006712887                                                           15    9605
XP_005246168                                                           15    9605
NP_570603                                                              15    9605
NP_055018                                                              15    9605
>NP_001136098
          Length = 274

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NEALTLKLY 9
           NE LTLKLY
Sbjct: 177 NETLTLKLY 185
>NP_000360
          Length = 764

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NEALTLKLY 9
           NE LTLKLY
Sbjct: 177 NETLTLKLY 185
>NP_001018046
          Length = 253

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NEALTLKLY 9
           NE LTLKLY
Sbjct: 177 NETLTLKLY 185
>XP_005268096
          Length = 253

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NEALTLKLY 9
           NE LTLKLY
Sbjct: 177 NETLTLKLY 185
>XP_006720308
          Length = 274

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NEALTLKLY 9
           NE LTLKLY
Sbjct: 177 NETLTLKLY 185
>XP_005268094
          Length = 764

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NEALTLKLY 9
           NE LTLKLY
Sbjct: 177 NETLTLKLY 185
>NP_000224
          Length = 699

 Score = 19.2 bits (38), Expect =   665
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   NEALTLKLY 9
           NE++TLKLY
Sbjct: 174 NESVTLKLY 182
>NP_006176
          Length = 2115

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   NEALTLKLY 9
           NE+LT++L+
Sbjct: 285 NESLTMRLH 293
>NP_001273490
          Length = 2101

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   NEALTLKLY 9
           NE+LT++L+
Sbjct: 285 NESLTMRLH 293
>XP_006718633
          Length = 2101

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   NEALTLKLY 9
           NE+LT++L+
Sbjct: 285 NESLTMRLH 293
>XP_006718632
          Length = 2115

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   NEALTLKLY 9
           NE+LT++L+
Sbjct: 285 NESLTMRLH 293
>XP_006718631
          Length = 2115

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   NEALTLKLY 9
           NE+LT++L+
Sbjct: 285 NESLTMRLH 293
>XP_006718630
          Length = 2115

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   NEALTLKLY 9
           NE+LT++L+
Sbjct: 285 NESLTMRLH 293
>XP_006718629
          Length = 2115

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   NEALTLKLY 9
           NE+LT++L+
Sbjct: 285 NESLTMRLH 293
>XP_006718628
          Length = 2115

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   NEALTLKLY 9
           NE+LT++L+
Sbjct: 285 NESLTMRLH 293
>XP_006718627
          Length = 2115

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   NEALTLKLY 9
           NE+LT++L+
Sbjct: 285 NESLTMRLH 293
>XP_006718626
          Length = 2115

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   NEALTLKLY 9
           NE+LT++L+
Sbjct: 285 NESLTMRLH 293
>XP_006718625
          Length = 2115

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   NEALTLKLY 9
           NE+LT++L+
Sbjct: 285 NESLTMRLH 293
>NP_444267
          Length = 529

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  NEALTLKL 8
          N+A TLKL
Sbjct: 67 NDAFTLKL 74
>XP_005265754
          Length = 412

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  NEALTLKL 8
          N+A TLKL
Sbjct: 67 NDAFTLKL 74
>NP_001193933
          Length = 335

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  NEALTLKL 8
          N+A TLKL
Sbjct: 67 NDAFTLKL 74
>NP_065170
          Length = 369

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   NEALTLKLY 9
           NE L  KLY
Sbjct: 119 NEGLNQKLY 127
>NP_001124335
          Length = 888

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   EALTLKLY 9
           E LTLK Y
Sbjct: 873 EILTLKTY 880
>NP_006200
          Length = 915

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   EALTLKLY 9
           E LTLK Y
Sbjct: 900 EILTLKTY 907
>NP_001035181
          Length = 863

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   EALTLKLY 9
           E LTLK Y
Sbjct: 848 EILTLKTY 855
>NP_003130
          Length = 638

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  NEALTLK 7
          +EALTLK
Sbjct: 49 HEALTLK 55
>NP_073728
          Length = 1255

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NEALTLK 7
           NEA TLK
Sbjct: 598 NEAATLK 604
>XP_006716650
          Length = 911

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   EALTLKLY 9
           E LTLK Y
Sbjct: 896 EILTLKTY 903
>XP_006716649
          Length = 936

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   EALTLKLY 9
           E LTLK Y
Sbjct: 921 EILTLKTY 928
>XP_006716648
          Length = 936

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   EALTLKLY 9
           E LTLK Y
Sbjct: 921 EILTLKTY 928
>XP_006716647
          Length = 940

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   EALTLKLY 9
           E LTLK Y
Sbjct: 925 EILTLKTY 932
>XP_006712887
          Length = 1255

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NEALTLK 7
           NEA TLK
Sbjct: 598 NEAATLK 604
>XP_005246168
          Length = 1255

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NEALTLK 7
           NEA TLK
Sbjct: 598 NEAATLK 604
>NP_570603
          Length = 955

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   EALTLKL 8
           +ALTLKL
Sbjct: 829 QALTLKL 835
>NP_055018
          Length = 977

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   EALTLKL 8
           +ALTLKL
Sbjct: 851 QALTLKL 857
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.314    0.132    0.338 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 351,696
Number of extensions: 372
Number of successful extensions: 35
Number of sequences better than 10000.0: 35
Number of HSP's gapped: 35
Number of HSP's successfully gapped: 35
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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