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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004295                                                              17    2528
NP_055224                                                              17    2528
NP_001020418                                                           17    2528
XP_005273157                                                           17    2528
XP_005273158                                                           17    2528
XP_005273156                                                           17    2528
NP_003255                                                              16    7355
NP_001540                                                              16    7355
NP_001364                                                              16    7355
NP_001276688                                                           16    7355
NP_001276687                                                           16    7355
NP_001026853                                                           16    7355
XP_005263250                                                           16    7355
XP_005263252                                                           16    7355
XP_005263253                                                           16    7355
XP_005263254                                                           16    7355
XP_005263251                                                           16    7355
XP_006711798                                                           16    7355
NP_001538                                                              16    7355
NP_005637                                                              15    9605
XP_005273291                                                           15    9605

>NP_004295
          Length = 1620

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NEAAIEILL 9
           NEAA EILL
Sbjct: 369 NEAAREILL 377



>NP_055224
          Length = 589

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   NEAAIEIL 8
           NEAA+E+L
Sbjct: 235 NEAALEVL 242



>NP_001020418
          Length = 325

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   NEAAIEIL 8
           NEAA+E+L
Sbjct: 235 NEAALEVL 242



>XP_005273157
          Length = 497

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   NEAAIEIL 8
           NEAA+E+L
Sbjct: 143 NEAALEVL 150



>XP_005273158
          Length = 497

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   NEAAIEIL 8
           NEAA+E+L
Sbjct: 143 NEAALEVL 150



>XP_005273156
          Length = 589

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   NEAAIEIL 8
           NEAA+E+L
Sbjct: 235 NEAALEVL 242



>NP_003255
          Length = 784

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   NEAAIEILL 9
           N+AAI ILL
Sbjct: 729 NDAAILILL 737



>NP_001540
          Length = 490

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  NEAAIEIL 8
          NEAA+E L
Sbjct: 67 NEAALECL 74



>NP_001364
          Length = 4515

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    NEAAIEILL 9
            NEAA+E +L
Sbjct: 1126 NEAALEKML 1134



>NP_001276688
          Length = 438

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  NEAAIEIL 8
          NEAA+E L
Sbjct: 15 NEAALECL 22



>NP_001276687
          Length = 438

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  NEAAIEIL 8
          NEAA+E L
Sbjct: 15 NEAALECL 22



>NP_001026853
          Length = 490

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  NEAAIEIL 8
          NEAA+E L
Sbjct: 67 NEAALECL 74



>XP_005263250
          Length = 784

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   NEAAIEILL 9
           N+AAI ILL
Sbjct: 729 NDAAILILL 737



>XP_005263252
          Length = 784

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   NEAAIEILL 9
           N+AAI ILL
Sbjct: 729 NDAAILILL 737



>XP_005263253
          Length = 784

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   NEAAIEILL 9
           N+AAI ILL
Sbjct: 729 NDAAILILL 737



>XP_005263254
          Length = 784

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   NEAAIEILL 9
           N+AAI ILL
Sbjct: 729 NDAAILILL 737



>XP_005263251
          Length = 784

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   NEAAIEILL 9
           N+AAI ILL
Sbjct: 729 NDAAILILL 737



>XP_006711798
          Length = 4586

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    NEAAIEILL 9
            NEAA+E +L
Sbjct: 1126 NEAALEKML 1134



>NP_001538
          Length = 472

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  NEAAIEIL 8
          NEAA+E L
Sbjct: 67 NEAALECL 74



>NP_005637
          Length = 1621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   EAAIEIL 8
           EAA+E+L
Sbjct: 138 EAAVEVL 144



>XP_005273291
          Length = 1620

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   EAAIEIL 8
           EAA+E+L
Sbjct: 138 EAAVEVL 144



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.315    0.136    0.316 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 331,233
Number of extensions: 446
Number of successful extensions: 21
Number of sequences better than 10000.0: 21
Number of HSP's gapped: 21
Number of HSP's successfully gapped: 21
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)