Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              19     869
NP_671714                                                              19     869
XP_006715890                                                           19     869
XP_005250160                                                           19     869
XP_005250159                                                           19     869
NP_065988                                                              16    5631
NP_001135791                                                           16    5631
NP_079479                                                              16    5631
XP_005267986                                                           16    5631
NP_001258828                                                           16    7355
NP_001258877                                                           16    7355
NP_858061                                                              16    7355
NP_858060                                                              16    7355
NP_001258829                                                           16    7355
NP_858064                                                              16    7355
NP_858063                                                              16    7355
NP_858062                                                              16    7355
NP_000391                                                              16    7355
NP_001171062                                                           16    7355
NP_002708                                                              16    7355
NP_060931                                                              16    7355
NP_001278239                                                           16    7355
NP_055467                                                              16    7355
NP_006026                                                              16    7355
XP_005258696                                                           16    7355
XP_005268287                                                           16    7355
XP_005268286                                                           16    7355
XP_005268285                                                           16    7355
XP_005268284                                                           16    7355
XP_006717978                                                           16    7355
XP_005277984                                                           16    7355
XP_005273617                                                           16    7355
XP_005273616                                                           16    7355
XP_003846672                                                           16    7355
NP_950251                                                              15    9605
NP_056315                                                              15    9605
NP_004422                                                              15    9605
NP_055742                                                              15    9605
NP_001162118                                                           15    9605
XP_005274525                                                           15    9605
XP_005261164                                                           15    9605
XP_005261166                                                           15    9605
XP_005261163                                                           15    9605
XP_005245809                                                           15    9605
XP_005245808                                                           15    9605
>NP_005742
          Length = 3907

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    NDEISVSSM 9
            +DEISVSSM
Sbjct: 1566 HDEISVSSM 1574
>NP_671714
          Length = 3899

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    NDEISVSSM 9
            +DEISVSSM
Sbjct: 1566 HDEISVSSM 1574
>XP_006715890
          Length = 3860

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    NDEISVSSM 9
            +DEISVSSM
Sbjct: 1566 HDEISVSSM 1574
>XP_005250160
          Length = 3939

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    NDEISVSSM 9
            +DEISVSSM
Sbjct: 1566 HDEISVSSM 1574
>XP_005250159
          Length = 3952

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    NDEISVSSM 9
            +DEISVSSM
Sbjct: 1566 HDEISVSSM 1574
>NP_065988
          Length = 2048

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   NDEISVSS 8
           NDE SVSS
Sbjct: 950 NDEKSVSS 957
>NP_001135791
          Length = 622

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NDEISVSS 8
           N E+SVSS
Sbjct: 270 NSEVSVSS 277
>NP_079479
          Length = 721

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NDEISVSS 8
           N E+SVSS
Sbjct: 270 NSEVSVSS 277
>XP_005267986
          Length = 2022

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   NDEISVSS 8
           NDE SVSS
Sbjct: 924 NDEKSVSS 931
>NP_001258828
          Length = 449

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DEISVSSM 9
           DEISV S+
Sbjct: 408 DEISVDSL 415
>NP_001258877
          Length = 432

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DEISVSSM 9
           DEISV S+
Sbjct: 391 DEISVDSL 398
>NP_858061
          Length = 549

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DEISVSSM 9
           DEISV S+
Sbjct: 508 DEISVDSL 515
>NP_858060
          Length = 509

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DEISVSSM 9
           DEISV S+
Sbjct: 468 DEISVDSL 475
>NP_001258829
          Length = 501

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DEISVSSM 9
           DEISV S+
Sbjct: 460 DEISVDSL 467
>NP_858064
          Length = 432

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DEISVSSM 9
           DEISV S+
Sbjct: 391 DEISVDSL 398
>NP_858063
          Length = 423

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DEISVSSM 9
           DEISV S+
Sbjct: 382 DEISVDSL 389
>NP_858062
          Length = 446

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DEISVSSM 9
           DEISV S+
Sbjct: 405 DEISVDSL 412
>NP_000391
          Length = 760

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   NDEISVSS 8
           ND++++SS
Sbjct: 499 NDQVAISS 506
>NP_001171062
          Length = 457

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DEISVSSM 9
           DEISV S+
Sbjct: 416 DEISVDSL 423
>NP_002708
          Length = 447

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DEISVSSM 9
           DEISV S+
Sbjct: 406 DEISVDSL 413
>NP_060931
          Length = 453

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DEISVSSM 9
           DEISV S+
Sbjct: 412 DEISVDSL 419
>NP_001278239
          Length = 288

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DEISVSSM 9
           DEISV S+
Sbjct: 247 DEISVDSL 254
>NP_055467
          Length = 963

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NDEISVSSM 9
           N E+SVSS+
Sbjct: 540 NPELSVSSV 548
>NP_006026
          Length = 1711

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    NDEISVSSM 9
            +DE SVSS+
Sbjct: 1151 DDEFSVSSV 1159
>XP_005258696
          Length = 736

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   NDEISVSS 8
           ND++++SS
Sbjct: 475 NDQVAISS 482
>XP_005268287
          Length = 1490

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    NDEISVSSM 9
            +DE SVSS+
Sbjct: 1168 DDEFSVSSV 1176
>XP_005268286
          Length = 1685

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    NDEISVSSM 9
            +DE SVSS+
Sbjct: 1125 DDEFSVSSV 1133
>XP_005268285
          Length = 1702

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    NDEISVSSM 9
            +DE SVSS+
Sbjct: 1142 DDEFSVSSV 1150
>XP_005268284
          Length = 1728

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    NDEISVSSM 9
            +DE SVSS+
Sbjct: 1168 DDEFSVSSV 1176
>XP_006717978
          Length = 226

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DEISVSSM 9
           DEISV S+
Sbjct: 185 DEISVDSL 192
>XP_005277984
          Length = 288

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DEISVSSM 9
           DEISV S+
Sbjct: 247 DEISVDSL 254
>XP_005273617
          Length = 288

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DEISVSSM 9
           DEISV S+
Sbjct: 247 DEISVDSL 254
>XP_005273616
          Length = 446

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DEISVSSM 9
           DEISV S+
Sbjct: 405 DEISVDSL 412
>XP_003846672
          Length = 830

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NDEISVSSM 9
           N E+SVSS+
Sbjct: 540 NPELSVSSV 548
>NP_950251
          Length = 541

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DEISVSSM 9
           DE+S SSM
Sbjct: 449 DELSESSM 456
>NP_056315
          Length = 696

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DEISVSSM 9
           DE+S SSM
Sbjct: 604 DELSESSM 611
>NP_004422
          Length = 976

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   DEISVSS 8
           DEI+VSS
Sbjct: 148 DEITVSS 154
>NP_055742
          Length = 1034

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    NDEISVSS 8
            ND +S+SS
Sbjct: 1007 NDPLSISS 1014
>NP_001162118
          Length = 1004

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NDEISVSS 8
           ND +S+SS
Sbjct: 977 NDPLSISS 984
>XP_005274525
          Length = 985

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NDEISVSS 8
           ND +S+SS
Sbjct: 958 NDPLSISS 965
>XP_005261164
          Length = 541

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DEISVSSM 9
           DE+S SSM
Sbjct: 449 DELSESSM 456
>XP_005261166
          Length = 541

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DEISVSSM 9
           DE+S SSM
Sbjct: 449 DELSESSM 456
>XP_005261163
          Length = 614

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DEISVSSM 9
           DE+S SSM
Sbjct: 522 DELSESSM 529
>XP_005245809
          Length = 922

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   DEISVSS 8
           DEI+VSS
Sbjct: 94  DEITVSS 100
>XP_005245808
          Length = 965

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   DEISVSS 8
           DEI+VSS
Sbjct: 137 DEITVSS 143
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.292    0.108    0.242 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 405,708
Number of extensions: 540
Number of successful extensions: 45
Number of sequences better than 10000.0: 45
Number of HSP's gapped: 45
Number of HSP's successfully gapped: 45
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.0 bits)
S2: 28 (15.4 bits)

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