Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004110                                                              20     390
XP_005266922                                                           18    1482
NP_001025029                                                           18    1482
NP_005520                                                              17    4312
NP_001263995                                                           17    4312
NP_001263994                                                           17    4312
XP_005246745                                                           17    4312
XP_006710661                                                           17    4312
XP_005245920                                                           17    4312
XP_006710660                                                           17    4312
XP_006710659                                                           17    4312
XP_006710658                                                           17    4312
XP_006710657                                                           17    4312
NP_001139317                                                           16    5631
NP_598334                                                              16    5631
NP_002932                                                              16    5631
NP_001161052                                                           16    5631
NP_055663                                                              16    5631
XP_005255055                                                           16    5631
XP_005255054                                                           16    5631
XP_006713341                                                           16    5631
XP_006713340                                                           16    5631
XP_006713339                                                           16    5631
NP_001073935                                                           16    7355
NP_001120916                                                           15    9605
NP_001001547                                                           15    9605
NP_000063                                                              15    9605
NP_006197                                                              15    9605
NP_001001548                                                           15    9605
NP_001120915                                                           15    9605
NP_002600                                                              15    9605
NP_000213                                                              15    9605
NP_001087241                                                           15    9605
NP_001276838                                                           15    9605
NP_001276837                                                           15    9605
NP_001276840                                                           15    9605
NP_653223                                                              15    9605
NP_116178                                                              15    9605
NP_004451                                                              15    9605
NP_001029345                                                           15    9605
NP_060639                                                              15    9605
XP_006719162                                                           15    9605
XP_005253462                                                           15    9605
XP_005251138                                                           15    9605
XP_005250772                                                           15    9605
XP_005250771                                                           15    9605
XP_005250770                                                           15    9605
XP_005268521                                                           15    9605
XP_006714104                                                           15    9605
XP_005265800                                                           15    9605
XP_006714103                                                           15    9605
XP_006714102                                                           15    9605
XP_005265799                                                           15    9605
XP_005265798                                                           15    9605
XP_005265797                                                           15    9605
>NP_004110
          Length = 993

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   NATAYGIS 8
           NATAYGIS
Sbjct: 626 NATAYGIS 633
>XP_005266922
          Length = 408

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   ATAYGISN 9
           AT YGISN
Sbjct: 227 ATLYGISN 234
>NP_001025029
          Length = 408

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   ATAYGISN 9
           AT YGISN
Sbjct: 227 ATLYGISN 234
>NP_005520
          Length = 4391

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    NATAYGISN 9
            N TA+GIS+
Sbjct: 3836 NLTAHGISH 3844
>NP_001263995
          Length = 218

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NATAYGISN 9
           N + YGISN
Sbjct: 144 NDSLYGISN 152
>NP_001263994
          Length = 218

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NATAYGISN 9
           N + YGISN
Sbjct: 144 NDSLYGISN 152
>XP_005246745
          Length = 244

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NATAYGISN 9
           N + YGISN
Sbjct: 170 NDSLYGISN 178
>XP_006710661
          Length = 4057

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    NATAYGISN 9
            N TA+GIS+
Sbjct: 4025 NLTAHGISH 4033
>XP_005245920
          Length = 4392

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    NATAYGISN 9
            N TA+GIS+
Sbjct: 3837 NLTAHGISH 3845
>XP_006710660
          Length = 4397

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    NATAYGISN 9
            N TA+GIS+
Sbjct: 3842 NLTAHGISH 3850
>XP_006710659
          Length = 4556

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    NATAYGISN 9
            N TA+GIS+
Sbjct: 4001 NLTAHGISH 4009
>XP_006710658
          Length = 4563

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    NATAYGISN 9
            N TA+GIS+
Sbjct: 4008 NLTAHGISH 4016
>XP_006710657
          Length = 4579

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    NATAYGISN 9
            N TA+GIS+
Sbjct: 4024 NLTAHGISH 4032
>NP_001139317
          Length = 1551

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ATAYGISN 9
           A AYGIS+
Sbjct: 601 ANAYGISD 608
>NP_598334
          Length = 1606

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ATAYGISN 9
           A AYGIS+
Sbjct: 601 ANAYGISD 608
>NP_002932
          Length = 1651

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ATAYGISN 9
           A AYGIS+
Sbjct: 637 ANAYGISD 644
>NP_001161052
          Length = 532

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ATAYGISN 9
           ATA+GI N
Sbjct: 473 ATAFGILN 480
>NP_055663
          Length = 683

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ATAYGISN 9
           ATA+GI N
Sbjct: 624 ATAFGILN 631
>XP_005255055
          Length = 683

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ATAYGISN 9
           ATA+GI N
Sbjct: 624 ATAFGILN 631
>XP_005255054
          Length = 683

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ATAYGISN 9
           ATA+GI N
Sbjct: 624 ATAFGILN 631
>XP_006713341
          Length = 1600

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ATAYGISN 9
           A AYGIS+
Sbjct: 586 ANAYGISD 593
>XP_006713340
          Length = 1615

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ATAYGISN 9
           A AYGIS+
Sbjct: 601 ANAYGISD 608
>XP_006713339
          Length = 1616

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ATAYGISN 9
           A AYGIS+
Sbjct: 602 ANAYGISD 609
>NP_001073935
          Length = 478

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ATAYGISN 9
           AT YG+S+
Sbjct: 142 ATKYGVSS 149
>NP_001120916
          Length = 472

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   NATAYGI 7
           N T+YG+
Sbjct: 321 NCTSYGV 327
>NP_001001547
          Length = 472

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   NATAYGI 7
           N T+YG+
Sbjct: 321 NCTSYGV 327
>NP_000063
          Length = 472

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   NATAYGI 7
           N T+YG+
Sbjct: 321 NCTSYGV 327
>NP_006197
          Length = 1089

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   TAYGIS 8
           TAYG+S
Sbjct: 611 TAYGLS 616
>NP_001001548
          Length = 472

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   NATAYGI 7
           N T+YG+
Sbjct: 321 NCTSYGV 327
>NP_001120915
          Length = 472

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   NATAYGI 7
           N T+YG+
Sbjct: 321 NCTSYGV 327
>NP_002600
          Length = 1106

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   ATAYGISN 9
           ATA+G+S+
Sbjct: 617 ATAHGLSH 624
>NP_000213
          Length = 976

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   ATAYGI 7
           ATAYG+
Sbjct: 606 ATAYGL 611
>NP_001087241
          Length = 972

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   ATAYGI 7
           ATAYG+
Sbjct: 602 ATAYGL 607
>NP_001276838
          Length = 412

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   NATAYGI 7
           N T+YG+
Sbjct: 261 NCTSYGV 267
>NP_001276837
          Length = 433

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   NATAYGI 7
           N T+YG+
Sbjct: 282 NCTSYGV 288
>NP_001276840
          Length = 396

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   NATAYGI 7
           N T+YG+
Sbjct: 245 NCTSYGV 251
>NP_653223
          Length = 773

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  ATAYGI 7
          ATAYG+
Sbjct: 37 ATAYGL 42
>NP_116178
          Length = 1025

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   NATAYG 6
           NAT YG
Sbjct: 910 NATVYG 915
>NP_004451
          Length = 760

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NATAYGIS 8
           NA+ YG+S
Sbjct: 99  NASNYGLS 106
>NP_001029345
          Length = 923

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   NATAYGISN 9
           N+T YG+++
Sbjct: 849 NSTEYGLAS 857
>NP_060639
          Length = 1747

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   NATAYGIS 8
           N TA GIS
Sbjct: 699 NTTAVGIS 706
>XP_006719162
          Length = 1676

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   NATAYGIS 8
           N TA GIS
Sbjct: 699 NTTAVGIS 706
>XP_005253462
          Length = 1747

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   NATAYGIS 8
           N TA GIS
Sbjct: 699 NTTAVGIS 706
>XP_005251138
          Length = 1037

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   NATAYG 6
           NAT YG
Sbjct: 922 NATVYG 927
>XP_005250772
          Length = 472

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   NATAYGI 7
           N T+YG+
Sbjct: 321 NCTSYGV 327
>XP_005250771
          Length = 472

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   NATAYGI 7
           N T+YG+
Sbjct: 321 NCTSYGV 327
>XP_005250770
          Length = 472

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   NATAYGI 7
           N T+YG+
Sbjct: 321 NCTSYGV 327
>XP_005268521
          Length = 1042

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   ATAYGISN 9
           ATA+G+S+
Sbjct: 553 ATAHGLSH 560
>XP_006714104
          Length = 832

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   TAYGIS 8
           TAYG+S
Sbjct: 636 TAYGLS 641
>XP_005265800
          Length = 1089

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   TAYGIS 8
           TAYG+S
Sbjct: 611 TAYGLS 616
>XP_006714103
          Length = 1111

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   TAYGIS 8
           TAYG+S
Sbjct: 633 TAYGLS 638
>XP_006714102
          Length = 1114

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   TAYGIS 8
           TAYG+S
Sbjct: 636 TAYGLS 641
>XP_005265799
          Length = 973

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   ATAYGI 7
           ATAYG+
Sbjct: 603 ATAYGL 608
>XP_005265798
          Length = 976

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   ATAYGI 7
           ATAYG+
Sbjct: 607 ATAYGL 612
>XP_005265797
          Length = 977

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   ATAYGI 7
           ATAYG+
Sbjct: 607 ATAYGL 612
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.302    0.116    0.315 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 419,107
Number of extensions: 726
Number of successful extensions: 55
Number of sequences better than 10000.0: 55
Number of HSP's gapped: 55
Number of HSP's successfully gapped: 55
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)

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