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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001167558                                                           18    1135
NP_858045                                                              18    1135
NP_006525                                                              18    1135
NP_001167559                                                           18    1135
NP_001191195                                                           17    4312
NP_001191196                                                           17    4312
NP_000800                                                              17    4312
XP_006714066                                                           17    4312
NP_694972                                                              16    5631
NP_001277144                                                           16    7355
NP_001277143                                                           16    7355
NP_001077383                                                           16    7355
NP_001320                                                              16    7355
NP_073713                                                              16    7355
XP_005269629                                                           16    7355
XP_005269628                                                           16    7355
XP_005269627                                                           16    7355
XP_005269626                                                           16    7355
XP_006717706                                                           16    7355
XP_005269625                                                           16    7355
XP_005269624                                                           16    7355
XP_006717705                                                           16    7355
XP_005269622                                                           16    7355
XP_005269621                                                           16    7355
XP_006717704                                                           16    7355
XP_005269619                                                           16    7355
XP_005269618                                                           16    7355
NP_001098991                                                           15    9605
XP_006717843                                                           15    9605
XP_005252734                                                           15    9605
XP_005252733                                                           15    9605
XP_005252732                                                           15    9605
XP_005252731                                                           15    9605
XP_005252730                                                           15    9605
XP_005252729                                                           15    9605
XP_006714304                                                           15    9605
NP_006230                                                              15    9605
NP_001736                                                              15    9605

>NP_001167558
          Length = 1423

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NASLEMRQR 9
           NAS EMRQR
Sbjct: 212 NASPEMRQR 220



>NP_858045
          Length = 1424

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NASLEMRQR 9
           NAS EMRQR
Sbjct: 212 NASPEMRQR 220



>NP_006525
          Length = 1420

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NASLEMRQR 9
           NAS EMRQR
Sbjct: 212 NASPEMRQR 220



>NP_001167559
          Length = 1415

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NASLEMRQR 9
           NAS EMRQR
Sbjct: 212 NASPEMRQR 220



>NP_001191195
          Length = 535

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NASLEMRQR 9
           NA+L MR+R
Sbjct: 362 NANLNMRKR 370



>NP_001191196
          Length = 484

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NASLEMRQR 9
           NA+L MR+R
Sbjct: 311 NANLNMRKR 319



>NP_000800
          Length = 554

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NASLEMRQR 9
           NA+L MR+R
Sbjct: 381 NANLNMRKR 389



>XP_006714066
          Length = 544

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NASLEMRQR 9
           NA+L MR+R
Sbjct: 371 NANLNMRKR 379



>NP_694972
          Length = 1330

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    SLEMRQR 9
            SLEMR+R
Sbjct: 1119 SLEMRER 1125



>NP_001277144
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ASLEMRQ 8
           ASLEMR+
Sbjct: 329 ASLEMRE 335



>NP_001277143
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ASLEMRQ 8
           ASLEMR+
Sbjct: 329 ASLEMRE 335



>NP_001077383
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ASLEMRQ 8
           ASLEMR+
Sbjct: 329 ASLEMRE 335



>NP_001320
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ASLEMRQ 8
           ASLEMR+
Sbjct: 329 ASLEMRE 335



>NP_073713
          Length = 985

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ASLEMRQ 8
           ASLEMR+
Sbjct: 869 ASLEMRE 875



>XP_005269629
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ASLEMRQ 8
           ASLEMR+
Sbjct: 329 ASLEMRE 335



>XP_005269628
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ASLEMRQ 8
           ASLEMR+
Sbjct: 329 ASLEMRE 335



>XP_005269627
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ASLEMRQ 8
           ASLEMR+
Sbjct: 329 ASLEMRE 335



>XP_005269626
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ASLEMRQ 8
           ASLEMR+
Sbjct: 329 ASLEMRE 335



>XP_006717706
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ASLEMRQ 8
           ASLEMR+
Sbjct: 329 ASLEMRE 335



>XP_005269625
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ASLEMRQ 8
           ASLEMR+
Sbjct: 329 ASLEMRE 335



>XP_005269624
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ASLEMRQ 8
           ASLEMR+
Sbjct: 329 ASLEMRE 335



>XP_006717705
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ASLEMRQ 8
           ASLEMR+
Sbjct: 329 ASLEMRE 335



>XP_005269622
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ASLEMRQ 8
           ASLEMR+
Sbjct: 329 ASLEMRE 335



>XP_005269621
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ASLEMRQ 8
           ASLEMR+
Sbjct: 329 ASLEMRE 335



>XP_006717704
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ASLEMRQ 8
           ASLEMR+
Sbjct: 329 ASLEMRE 335



>XP_005269619
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ASLEMRQ 8
           ASLEMR+
Sbjct: 329 ASLEMRE 335



>XP_005269618
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ASLEMRQ 8
           ASLEMR+
Sbjct: 329 ASLEMRE 335



>NP_001098991
          Length = 844

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SLEMRQR 9
           ++EMRQR
Sbjct: 369 NIEMRQR 375



>XP_006717843
          Length = 844

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SLEMRQR 9
           ++EMRQR
Sbjct: 369 NIEMRQR 375



>XP_005252734
          Length = 846

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SLEMRQR 9
           ++EMRQR
Sbjct: 369 NIEMRQR 375



>XP_005252733
          Length = 916

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SLEMRQR 9
           ++EMRQR
Sbjct: 295 NIEMRQR 301



>XP_005252732
          Length = 976

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SLEMRQR 9
           ++EMRQR
Sbjct: 369 NIEMRQR 375



>XP_005252731
          Length = 988

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SLEMRQR 9
           ++EMRQR
Sbjct: 369 NIEMRQR 375



>XP_005252730
          Length = 990

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SLEMRQR 9
           ++EMRQR
Sbjct: 369 NIEMRQR 375



>XP_005252729
          Length = 990

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SLEMRQR 9
           ++EMRQR
Sbjct: 369 NIEMRQR 375



>XP_006714304
          Length = 598

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  ASLEMRQR 9
          A LEMR+R
Sbjct: 35 ARLEMRRR 42



>NP_006230
          Length = 753

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  ASLEMRQR 9
          A LEMR+R
Sbjct: 35 ARLEMRRR 42



>NP_001736
          Length = 296

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NASLEMRQR 9
           + +LE+RQR
Sbjct: 126 DVNLELRQR 134



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.315    0.118    0.283 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 369,272
Number of extensions: 343
Number of successful extensions: 38
Number of sequences better than 10000.0: 38
Number of HSP's gapped: 38
Number of HSP's successfully gapped: 38
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)