Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_444256                                                              19     869
NP_444254                                                              19     869
NP_444253                                                              19     869
NP_444255                                                              19     869
XP_005247551                                                           19     869
XP_005247550                                                           19     869
XP_006713712                                                           19     869
XP_005247549                                                           19     869
NP_002609                                                              17    3301
NP_000319                                                              16    7355
NP_940969                                                              16    7355
XP_005272690                                                           16    7355
XP_006724601                                                           16    7355
XP_005265966                                                           16    7355
XP_006712581                                                           15    9605
XP_005246132                                                           15    9605
NP_001073973                                                           15    9605
>NP_444256
          Length = 1794

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NAKTVESSK 9
           NAK VESSK
Sbjct: 935 NAKAVESSK 943
>NP_444254
          Length = 1845

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NAKTVESSK 9
           NAK VESSK
Sbjct: 935 NAKAVESSK 943
>NP_444253
          Length = 1914

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NAKTVESSK 9
            NAK VESSK
Sbjct: 1004 NAKAVESSK 1012
>NP_444255
          Length = 1863

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NAKTVESSK 9
            NAK VESSK
Sbjct: 1004 NAKAVESSK 1012
>XP_005247551
          Length = 1855

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NAKTVESSK 9
           NAK VESSK
Sbjct: 945 NAKAVESSK 953
>XP_005247550
          Length = 1856

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NAKTVESSK 9
            NAK VESSK
Sbjct: 1014 NAKAVESSK 1022
>XP_006713712
          Length = 1923

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NAKTVESSK 9
            NAK VESSK
Sbjct: 1014 NAKAVESSK 1022
>XP_005247549
          Length = 1924

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NAKTVESSK 9
            NAK VESSK
Sbjct: 1014 NAKAVESSK 1022
>NP_002609
          Length = 403

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   KTVESSK 9
           KTVESSK
Sbjct: 341 KTVESSK 347
>NP_000319
          Length = 815

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NAKTVESSK 9
           NA+ VES K
Sbjct: 655 NAENVESKK 663
>NP_940969
          Length = 457

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NAKTVE 6
           NAKTVE
Sbjct: 126 NAKTVE 131
>XP_005272690
          Length = 704

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NAKTVESSK 9
           NA+ VES K
Sbjct: 544 NAENVESKK 552
>XP_006724601
          Length = 1020

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NAKTVESSK 9
           NA+ VES K
Sbjct: 860 NAENVESKK 868
>XP_005265966
          Length = 891

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NAKTVE 6
           NAKTVE
Sbjct: 560 NAKTVE 565
>XP_006712581
          Length = 1047

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NAKTVESSK 9
           N K +ES+K
Sbjct: 963 NCKQIESAK 971
>XP_005246132
          Length = 1047

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NAKTVESSK 9
           N K +ES+K
Sbjct: 963 NCKQIESAK 971
>NP_001073973
          Length = 1052

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NAKTVESSK 9
           N K +ES+K
Sbjct: 968 NCKQIESAK 976
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.273   0.0907    0.194 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 230,776
Number of extensions: 184
Number of successful extensions: 17
Number of sequences better than 10000.0: 17
Number of HSP's gapped: 17
Number of HSP's successfully gapped: 17
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 18 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.5 bits)
S2: 28 (15.4 bits)

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