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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001155973                                                           18    1482
NP_001020014                                                           18    1482
NP_055903                                                              18    1482
NP_071332                                                              16    5631
NP_002009                                                              16    7355
NP_001243193                                                           16    7355
NP_001243194                                                           16    7355
XP_005256615                                                           16    7355
XP_005256613                                                           16    7355
XP_005256612                                                           16    7355
NP_003119                                                              15    9605
XP_005264575                                                           15    9605
XP_006712150                                                           15    9605
XP_005264574                                                           15    9605
NP_842565                                                              15    9605

>NP_001155973
          Length = 1833

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  NAKRVAVLN 9
          NAKRVAV N
Sbjct: 69 NAKRVAVPN 77



>NP_001020014
          Length = 1029

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NAKRVAVLN 9
           NAKRVAV N
Sbjct: 105 NAKRVAVPN 113



>NP_055903
          Length = 1723

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  NAKRVAVLN 9
          NAKRVAV N
Sbjct: 69 NAKRVAVPN 77



>NP_071332
          Length = 384

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NAKRVAVLN 9
           + KRV+VLN
Sbjct: 222 SVKRVSVLN 230



>NP_002009
          Length = 1269

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NAKRVAVLN 9
           NAK + VLN
Sbjct: 125 NAKNMLVLN 133



>NP_001243193
          Length = 1258

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NAKRVAVLN 9
           NAK + VLN
Sbjct: 114 NAKNMLVLN 122



>NP_001243194
          Length = 1214

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NAKRVAVLN 9
           NAK + VLN
Sbjct: 125 NAKNMLVLN 133



>XP_005256615
          Length = 1268

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NAKRVAVLN 9
           NAK + VLN
Sbjct: 125 NAKNMLVLN 133



>XP_005256613
          Length = 1295

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NAKRVAVLN 9
           NAK + VLN
Sbjct: 125 NAKNMLVLN 133



>XP_005256612
          Length = 1296

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NAKRVAVLN 9
           NAK + VLN
Sbjct: 125 NAKNMLVLN 133



>NP_003119
          Length = 2364

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AKRVAVLN 9
           A RVAV+N
Sbjct: 902 ASRVAVVN 909



>XP_005264575
          Length = 2351

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AKRVAVLN 9
           A RVAV+N
Sbjct: 889 ASRVAVVN 896



>XP_006712150
          Length = 2364

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AKRVAVLN 9
           A RVAV+N
Sbjct: 902 ASRVAVVN 909



>XP_005264574
          Length = 2364

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AKRVAVLN 9
           A RVAV+N
Sbjct: 902 ASRVAVVN 909



>NP_842565
          Length = 2155

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AKRVAVLN 9
           A RVAV+N
Sbjct: 889 ASRVAVVN 896



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.317    0.124    0.308 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 286,998
Number of extensions: 413
Number of successful extensions: 15
Number of sequences better than 10000.0: 15
Number of HSP's gapped: 15
Number of HSP's successfully gapped: 15
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)