Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_444256                                                              19     665
NP_444254                                                              19     665
NP_444253                                                              19     665
NP_444255                                                              19     665
NP_444260                                                              19     665
NP_444259                                                              19     665
XP_005247551                                                           19     665
XP_005247550                                                           19     665
XP_006713712                                                           19     665
XP_005247549                                                           19     665
NP_001131022                                                           17    3301
NP_003119                                                              17    3301
XP_006712913                                                           17    3301
XP_005246199                                                           17    3301
XP_005246198                                                           17    3301
XP_005246197                                                           17    3301
XP_005246196                                                           17    3301
XP_005246195                                                           17    3301
XP_005246194                                                           17    3301
XP_005264575                                                           17    3301
XP_006712150                                                           17    3301
XP_005264574                                                           17    3301
NP_001131023                                                           17    3301
NP_842565                                                              17    3301
NP_001269620                                                           17    4312
NP_001127841                                                           17    4312
NP_006300                                                              17    4312
NP_060194                                                              17    4312
NP_694580                                                              17    4312
XP_006713467                                                           17    4312
XP_005265614                                                           17    4312
XP_005265613                                                           17    4312
XP_005265612                                                           17    4312
XP_005265611                                                           17    4312
XP_006713465                                                           17    4312
XP_005265610                                                           17    4312
XP_006713464                                                           17    4312
XP_006713463                                                           17    4312
XP_006713462                                                           17    4312
XP_005265608                                                           17    4312
XP_005265607                                                           17    4312
XP_006713461                                                           17    4312
XP_006713460                                                           17    4312
XP_006713459                                                           17    4312
XP_006713458                                                           17    4312
XP_006713457                                                           17    4312
XP_006713456                                                           17    4312
XP_006713455                                                           17    4312
XP_006713454                                                           17    4312
XP_006713453                                                           17    4312
XP_006713452                                                           17    4312
XP_006713451                                                           17    4312
XP_006713450                                                           17    4312
XP_006713449                                                           17    4312
XP_006713448                                                           17    4312
XP_005265597                                                           17    4312
XP_005265596                                                           17    4312
XP_005265595                                                           17    4312
NP_065944                                                              16    5631
XP_005272199                                                           16    7355
NP_055644                                                              16    7355
NP_001265612                                                           15    9605
NP_057285                                                              15    9605
NP_001265614                                                           15    9605
NP_071400                                                              15    9605
NP_005642                                                              15    9605
NP_001028774                                                           15    9605
NP_060692                                                              15    9605
NP_775919                                                              15    9605
XP_006724655                                                           15    9605
XP_005253072                                                           15    9605
XP_006713718                                                           15    9605
XP_006713717                                                           15    9605
XP_005247577                                                           15    9605
XP_006712503                                                           15    9605
>NP_444256
          Length = 1794

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    NAEKLESE 8
            NAEKLESE
Sbjct: 1662 NAEKLESE 1669
>NP_444254
          Length = 1845

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    NAEKLESE 8
            NAEKLESE
Sbjct: 1713 NAEKLESE 1720
>NP_444253
          Length = 1914

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    NAEKLESE 8
            NAEKLESE
Sbjct: 1782 NAEKLESE 1789
>NP_444255
          Length = 1863

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    NAEKLESE 8
            NAEKLESE
Sbjct: 1731 NAEKLESE 1738
>NP_444260
          Length = 154

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1  NAEKLESE 8
          NAEKLESE
Sbjct: 22 NAEKLESE 29
>NP_444259
          Length = 153

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1  NAEKLESE 8
          NAEKLESE
Sbjct: 22 NAEKLESE 29
>XP_005247551
          Length = 1855

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    NAEKLESE 8
            NAEKLESE
Sbjct: 1723 NAEKLESE 1730
>XP_005247550
          Length = 1856

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    NAEKLESE 8
            NAEKLESE
Sbjct: 1724 NAEKLESE 1731
>XP_006713712
          Length = 1923

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    NAEKLESE 8
            NAEKLESE
Sbjct: 1792 NAEKLESE 1799
>XP_005247549
          Length = 1924

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    NAEKLESE 8
            NAEKLESE
Sbjct: 1792 NAEKLESE 1799
>NP_001131022
          Length = 640

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 586 NAEKIEDE 593
>NP_003119
          Length = 2364

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2    AEKLESE 8
            AEKLESE
Sbjct: 1019 AEKLESE 1025
>XP_006712913
          Length = 648

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 594 NAEKIEDE 601
>XP_005246199
          Length = 404

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 350 NAEKIEDE 357
>XP_005246198
          Length = 428

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 374 NAEKIEDE 381
>XP_005246197
          Length = 575

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 521 NAEKIEDE 528
>XP_005246196
          Length = 599

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 545 NAEKIEDE 552
>XP_005246195
          Length = 625

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 607 NAEKIEDE 614
>XP_005246194
          Length = 661

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 607 NAEKIEDE 614
>XP_005264575
          Length = 2351

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2    AEKLESE 8
            AEKLESE
Sbjct: 1006 AEKLESE 1012
>XP_006712150
          Length = 2364

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2    AEKLESE 8
            AEKLESE
Sbjct: 1019 AEKLESE 1025
>XP_005264574
          Length = 2364

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2    AEKLESE 8
            AEKLESE
Sbjct: 1019 AEKLESE 1025
>NP_001131023
          Length = 394

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 340 NAEKIEDE 347
>NP_842565
          Length = 2155

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2    AEKLESE 8
            AEKLESE
Sbjct: 1006 AEKLESE 1012
>NP_001269620
          Length = 503

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 449 NAEKVEDE 456
>NP_001127841
          Length = 424

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 370 NAEKVEDE 377
>NP_006300
          Length = 721

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 667 NAEKVEDE 674
>NP_060194
          Length = 400

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 346 NAEKVEDE 353
>NP_694580
          Length = 208

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   NAEKLESEA 9
           NA+KL SEA
Sbjct: 134 NAQKLLSEA 142
>XP_006713467
          Length = 415

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 361 NAEKVEDE 368
>XP_005265614
          Length = 465

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 411 NAEKVEDE 418
>XP_005265613
          Length = 465

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 411 NAEKVEDE 418
>XP_005265612
          Length = 489

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 435 NAEKVEDE 442
>XP_005265611
          Length = 489

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 435 NAEKVEDE 442
>XP_006713465
          Length = 489

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 435 NAEKVEDE 442
>XP_005265610
          Length = 489

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 435 NAEKVEDE 442
>XP_006713464
          Length = 504

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 450 NAEKVEDE 457
>XP_006713463
          Length = 504

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 450 NAEKVEDE 457
>XP_006713462
          Length = 505

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 451 NAEKVEDE 458
>XP_005265608
          Length = 540

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 486 NAEKVEDE 493
>XP_005265607
          Length = 577

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 523 NAEKVEDE 530
>XP_006713461
          Length = 625

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 571 NAEKVEDE 578
>XP_006713460
          Length = 649

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 595 NAEKVEDE 602
>XP_006713459
          Length = 685

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 631 NAEKVEDE 638
>XP_006713458
          Length = 690

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 636 NAEKVEDE 643
>XP_006713457
          Length = 690

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 636 NAEKVEDE 643
>XP_006713456
          Length = 691

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 637 NAEKVEDE 644
>XP_006713455
          Length = 697

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 643 NAEKVEDE 650
>XP_006713454
          Length = 698

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 644 NAEKVEDE 651
>XP_006713453
          Length = 704

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 650 NAEKVEDE 657
>XP_006713452
          Length = 704

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 650 NAEKVEDE 657
>XP_006713451
          Length = 705

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 651 NAEKVEDE 658
>XP_006713450
          Length = 706

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 652 NAEKVEDE 659
>XP_006713449
          Length = 707

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 653 NAEKVEDE 660
>XP_006713448
          Length = 720

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 666 NAEKVEDE 673
>XP_005265597
          Length = 721

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 667 NAEKVEDE 674
>XP_005265596
          Length = 721

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 667 NAEKVEDE 674
>XP_005265595
          Length = 721

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAEKLESE 8
           NAEK+E E
Sbjct: 667 NAEKVEDE 674
>NP_065944
          Length = 1701

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    NAEKLESE 8
            N EKLE+E
Sbjct: 1082 NMEKLENE 1089
>XP_005272199
          Length = 1032

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NAEKLE 6
           NAEKLE
Sbjct: 845 NAEKLE 850
>NP_055644
          Length = 1031

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NAEKLE 6
           NAEKLE
Sbjct: 845 NAEKLE 850
>NP_001265612
          Length = 837

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   EKLESE 8
           EKLESE
Sbjct: 404 EKLESE 409
>NP_057285
          Length = 780

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   EKLESE 8
           EKLESE
Sbjct: 346 EKLESE 351
>NP_001265614
          Length = 777

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   EKLESE 8
           EKLESE
Sbjct: 346 EKLESE 351
>NP_071400
          Length = 693

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   NAEKLES 7
           NAE++ES
Sbjct: 208 NAERMES 214
>NP_005642
          Length = 406

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  NAEKLESE 8
          NA++L+SE
Sbjct: 53 NAQELQSE 60
>NP_001028774
          Length = 1379

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   NAEKLES 7
           NA+K+ES
Sbjct: 525 NADKIES 531
>NP_060692
          Length = 412

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   EKLESE 8
           EKLESE
Sbjct: 174 EKLESE 179
>NP_775919
          Length = 1530

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   EKLESEA 9
           EKL+SEA
Sbjct: 306 EKLKSEA 312
>XP_006724655
          Length = 694

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   NAEKLES 7
           NAE++ES
Sbjct: 208 NAERMES 214
>XP_005253072
          Length = 328

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  EKLESE 8
          EKLESE
Sbjct: 90 EKLESE 95
>XP_006713718
          Length = 779

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   EKLESE 8
           EKLESE
Sbjct: 346 EKLESE 351
>XP_006713717
          Length = 779

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   EKLESE 8
           EKLESE
Sbjct: 346 EKLESE 351
>XP_005247577
          Length = 780

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   EKLESE 8
           EKLESE
Sbjct: 346 EKLESE 351
>XP_006712503
          Length = 1470

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   EKLESEA 9
           EKL+SEA
Sbjct: 306 EKLKSEA 312
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.274   0.0983    0.211 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 482,058
Number of extensions: 920
Number of successful extensions: 75
Number of sequences better than 10000.0: 75
Number of HSP's gapped: 75
Number of HSP's successfully gapped: 75
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 18 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.4 bits)
S2: 28 (15.4 bits)

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