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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              20     509
NP_001075109                                                           20     509
NP_001127907                                                           16    5631
NP_115639                                                              16    5631
XP_006713848                                                           16    5631
XP_006713847                                                           16    5631
NP_387510                                                              16    7355
NP_001247403                                                           16    7355
NP_061139                                                              16    7355
XP_006726460                                                           16    7355
XP_006724022                                                           16    7355
XP_005275982                                                           16    7355
NP_078904                                                              15    9605
NP_001182412                                                           15    9605
XP_005248180                                                           15    9605
XP_006714090                                                           15    9605
NP_003206                                                              15    9605

>NP_008835
          Length = 4128

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MYDIIAAEK 9
            M+DIIAAEK
Sbjct: 1303 MHDIIAAEK 1311



>NP_001075109
          Length = 4097

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MYDIIAAEK 9
            M+DIIAAEK
Sbjct: 1303 MHDIIAAEK 1311



>NP_001127907
          Length = 443

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   YDIIAAEK 9
           YDIIA E+
Sbjct: 210 YDIIAREE 217



>NP_115639
          Length = 443

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   YDIIAAEK 9
           YDIIA E+
Sbjct: 210 YDIIAREE 217



>XP_006713848
          Length = 345

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   YDIIAAEK 9
           YDIIA E+
Sbjct: 112 YDIIAREE 119



>XP_006713847
          Length = 369

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   YDIIAAEK 9
           YDIIA E+
Sbjct: 136 YDIIAREE 143



>NP_387510
          Length = 412

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  MYDIIAAEK 9
          +YD  AAEK
Sbjct: 37 IYDCSAAEK 45



>NP_001247403
          Length = 411

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  MYDIIAAEK 9
          +YD  AAEK
Sbjct: 37 IYDCSAAEK 45



>NP_061139
          Length = 412

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  MYDIIAAEK 9
          +YD  AAEK
Sbjct: 37 IYDCSAAEK 45



>XP_006726460
          Length = 247

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  YDIIAAEK 9
          YD++AA K
Sbjct: 92 YDLVAAGK 99



>XP_006724022
          Length = 412

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  MYDIIAAEK 9
          +YD  AAEK
Sbjct: 37 IYDCSAAEK 45



>XP_005275982
          Length = 187

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  YDIIAAEK 9
          YD++AA K
Sbjct: 32 YDLVAAGK 39



>NP_078904
          Length = 714

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   YDIIAAEK 9
           YD + AEK
Sbjct: 297 YDFLIAEK 304



>NP_001182412
          Length = 714

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   YDIIAAEK 9
           YD + AEK
Sbjct: 297 YDFLIAEK 304



>XP_005248180
          Length = 551

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   MYDIIAAE 8
           MYD  AAE
Sbjct: 107 MYDFQAAE 114



>XP_006714090
          Length = 551

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   MYDIIAAE 8
           MYD  AAE
Sbjct: 107 MYDFQAAE 114



>NP_003206
          Length = 631

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   MYDIIAAE 8
           MYD  AAE
Sbjct: 187 MYDFQAAE 194



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.323    0.135    0.351 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 369,396
Number of extensions: 295
Number of successful extensions: 17
Number of sequences better than 10000.0: 17
Number of HSP's gapped: 17
Number of HSP's successfully gapped: 17
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)