Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001129490                                                           17    2528
NP_001278092                                                           17    2528
NP_000111                                                              17    2528
XP_006720531                                                           17    3301
XP_005254344                                                           17    3301
NP_056312                                                              16    5631
NP_001273416                                                           16    5631
NP_001005169                                                           16    5631
NP_001005170                                                           16    5631
NP_000584                                                              16    7355
NP_078966                                                              16    7355
NP_001231800                                                           16    7355
NP_001334                                                              16    7355
NP_000258                                                              16    7355
NP_001035957                                                           16    7355
NP_005572                                                              16    7355
NP_056362                                                              16    7355
NP_001013275                                                           16    7355
XP_006721991                                                           16    7355
XP_006721990                                                           16    7355
XP_006721989                                                           16    7355
XP_005258041                                                           16    7355
XP_006721988                                                           16    7355
XP_006721987                                                           16    7355
XP_006721986                                                           16    7355
XP_005258040                                                           16    7355
XP_006721985                                                           16    7355
XP_006716315                                                           16    7355
XP_006710090                                                           16    7355
NP_001269117                                                           15    9605
NP_001269116                                                           15    9605
NP_001269115                                                           15    9605
NP_006630                                                              15    9605
NP_579875                                                              15    9605
NP_758847                                                              15    9605
NP_775796                                                              15    9605
XP_005260679                                                           15    9605
XP_006711370                                                           15    9605
NP_001119601                                                           15    9605
NP_055557                                                              15    9605
>NP_001129490
          Length = 455

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1 MWISISLTS 9
         MW+ I LTS
Sbjct: 1 MWLEILLTS 9
>NP_001278092
          Length = 455

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1 MWISISLTS 9
         MW+ I LTS
Sbjct: 1 MWLEILLTS 9
>NP_000111
          Length = 455

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1 MWISISLTS 9
         MW+ I LTS
Sbjct: 1 MWLEILLTS 9
>XP_006720531
          Length = 415

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   MWISISLT 8
           +WI ISLT
Sbjct: 236 LWIFISLT 243
>XP_005254344
          Length = 556

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   MWISISLT 8
           +WI ISLT
Sbjct: 377 LWIFISLT 384
>NP_056312
          Length = 555

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   WISISLT 8
           WI ISLT
Sbjct: 377 WIFISLT 383
>NP_001273416
          Length = 494

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   WISISLT 8
           WI ISLT
Sbjct: 316 WIFISLT 322
>NP_001005169
          Length = 324

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1  MWISISLTS 9
          +W++ISL++
Sbjct: 29 LWLAISLSA 37
>NP_001005170
          Length = 350

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1  MWISISLTS 9
          +W++ISL++
Sbjct: 55 LWLAISLSA 63
>NP_000584
          Length = 808

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   MWISISLT 8
           +W S+SLT
Sbjct: 381 LWGSVSLT 388
>NP_078966
          Length = 14507

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    WISISLT 8
            W+S SLT
Sbjct: 1460 WLSTSLT 1466
>NP_001231800
          Length = 749

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   MWISISLT 8
           +W++ISL+
Sbjct: 93  IWVNISLS 100
>NP_001334
          Length = 770

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   MWISISLT 8
           +W++ISL+
Sbjct: 93  IWVNISLS 100
>NP_000258
          Length = 2818

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    WISISLTS 9
            W S++LTS
Sbjct: 1538 WSSLNLTS 1545
>NP_001035957
          Length = 2839

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    WISISLTS 9
            W S++LTS
Sbjct: 1559 WSSLNLTS 1566
>NP_005572
          Length = 628

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   WISISLT 8
           W+S SLT
Sbjct: 502 WVSSSLT 508
>NP_056362
          Length = 607

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 2   WISISLT 8
           W+ +SLT
Sbjct: 546 WVRVSLT 552
>NP_001013275
          Length = 588

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   WISISLT 8
           W+S SLT
Sbjct: 502 WVSSSLT 508
>XP_006721991
          Length = 1632

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    WISISLTS 9
            W S++LTS
Sbjct: 1569 WSSLNLTS 1576
>XP_006721990
          Length = 2823

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    WISISLTS 9
            W S++LTS
Sbjct: 1569 WSSLNLTS 1576
>XP_006721989
          Length = 2823

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    WISISLTS 9
            W S++LTS
Sbjct: 1569 WSSLNLTS 1576
>XP_005258041
          Length = 2836

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    WISISLTS 9
            W S++LTS
Sbjct: 1538 WSSLNLTS 1545
>XP_006721988
          Length = 2846

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    WISISLTS 9
            W S++LTS
Sbjct: 1548 WSSLNLTS 1555
>XP_006721987
          Length = 2849

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    WISISLTS 9
            W S++LTS
Sbjct: 1569 WSSLNLTS 1576
>XP_006721986
          Length = 2854

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    WISISLTS 9
            W S++LTS
Sbjct: 1556 WSSLNLTS 1563
>XP_005258040
          Length = 2857

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    WISISLTS 9
            W S++LTS
Sbjct: 1559 WSSLNLTS 1566
>XP_006721985
          Length = 2867

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    WISISLTS 9
            W S++LTS
Sbjct: 1569 WSSLNLTS 1576
>XP_006716315
          Length = 442

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   WISISLT 8
           W S+SLT
Sbjct: 412 WSSVSLT 418
>XP_006710090
          Length = 255

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  MWISISLT 8
          +WI +SLT
Sbjct: 3  IWIFLSLT 10
>NP_001269117
          Length = 337

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   MWISISLTS 9
           +WI + LTS
Sbjct: 147 IWIFVILTS 155
>NP_001269116
          Length = 337

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   MWISISLTS 9
           +WI + LTS
Sbjct: 147 IWIFVILTS 155
>NP_001269115
          Length = 337

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   MWISISLTS 9
           +WI + LTS
Sbjct: 147 IWIFVILTS 155
>NP_006630
          Length = 337

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   MWISISLTS 9
           +WI + LTS
Sbjct: 147 IWIFVILTS 155
>NP_579875
          Length = 436

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   WISISLTS 9
           W+ IS+T+
Sbjct: 366 WVIISMTT 373
>NP_758847
          Length = 425

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   WISISLTS 9
           W+ IS+T+
Sbjct: 355 WVIISMTT 362
>NP_775796
          Length = 333

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   WISISLTS 9
           W+ + LTS
Sbjct: 170 WLQLGLTS 177
>XP_005260679
          Length = 625

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 1   MWISISL 7
           MW  ISL
Sbjct: 438 MWFGISL 444
>XP_006711370
          Length = 232

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   MWISISLT 8
           +W SISL+
Sbjct: 219 IWTSISLS 226
>NP_001119601
          Length = 255

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  MWISISLTS 9
          +W++ +LTS
Sbjct: 76 IWLTAALTS 84
>NP_055557
          Length = 642

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 1   MWISISL 7
           MW  ISL
Sbjct: 455 MWFGISL 461
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.322    0.120    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 479,523
Number of extensions: 443
Number of successful extensions: 40
Number of sequences better than 10000.0: 40
Number of HSP's gapped: 40
Number of HSP's successfully gapped: 40
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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