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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              18    1135
NP_001075109                                                           18    1135
NP_065783                                                              17    4312
NP_059989                                                              17    4312
XP_006715590                                                           17    4312
XP_005267126                                                           17    4312
XP_006715589                                                           17    4312
XP_006715588                                                           17    4312
XP_006715587                                                           17    4312
XP_006715586                                                           17    4312
NP_001026                                                              15    9605
XP_006714629                                                           15    9605
XP_006711873                                                           15    9605
XP_006711871                                                           15    9605
XP_006711870                                                           15    9605
XP_006711869                                                           15    9605
XP_006711868                                                           15    9605
XP_006711867                                                           15    9605
XP_006711866                                                           15    9605
XP_006711865                                                           15    9605

>NP_008835
          Length = 4128

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    MVSAVLNSM 9
            MVSAVLN M
Sbjct: 1592 MVSAVLNGM 1600



>NP_001075109
          Length = 4097

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    MVSAVLNSM 9
            MVSAVLN M
Sbjct: 1592 MVSAVLNGM 1600



>NP_065783
          Length = 2249

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    VSAVLNSM 9
            +SAVLNS+
Sbjct: 2227 ISAVLNSL 2234



>NP_059989
          Length = 2236

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    VSAVLNSM 9
            +SAVLNS+
Sbjct: 2214 ISAVLNSL 2221



>XP_006715590
          Length = 2277

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    VSAVLNSM 9
            +SAVLNS+
Sbjct: 2255 ISAVLNSL 2262



>XP_005267126
          Length = 2316

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    VSAVLNSM 9
            +SAVLNS+
Sbjct: 2294 ISAVLNSL 2301



>XP_006715589
          Length = 2320

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    VSAVLNSM 9
            +SAVLNS+
Sbjct: 2298 ISAVLNSL 2305



>XP_006715588
          Length = 2330

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    VSAVLNSM 9
            +SAVLNS+
Sbjct: 2308 ISAVLNSL 2315



>XP_006715587
          Length = 2359

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    VSAVLNSM 9
            +SAVLNS+
Sbjct: 2337 ISAVLNSL 2344



>XP_006715586
          Length = 2373

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    VSAVLNSM 9
            +SAVLNS+
Sbjct: 2351 ISAVLNSL 2358



>NP_001026
          Length = 4967

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    VSAVLNSM 9
            +SAVLNS+
Sbjct: 4694 LSAVLNSI 4701



>XP_006714629
          Length = 409

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  SAVLNSM 9
          SAVLN+M
Sbjct: 31 SAVLNAM 37



>XP_006711873
          Length = 4954

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    VSAVLNSM 9
            +SAVLNS+
Sbjct: 4681 LSAVLNSI 4688



>XP_006711871
          Length = 4906

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    VSAVLNSM 9
            +SAVLNS+
Sbjct: 4633 LSAVLNSI 4640



>XP_006711870
          Length = 4965

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    VSAVLNSM 9
            +SAVLNS+
Sbjct: 4692 LSAVLNSI 4699



>XP_006711869
          Length = 4973

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    VSAVLNSM 9
            +SAVLNS+
Sbjct: 4700 LSAVLNSI 4707



>XP_006711868
          Length = 4975

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    VSAVLNSM 9
            +SAVLNS+
Sbjct: 4702 LSAVLNSI 4709



>XP_006711867
          Length = 4977

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    VSAVLNSM 9
            +SAVLNS+
Sbjct: 4704 LSAVLNSI 4711



>XP_006711866
          Length = 4984

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    VSAVLNSM 9
            +SAVLNS+
Sbjct: 4711 LSAVLNSI 4718



>XP_006711865
          Length = 4985

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    VSAVLNSM 9
            +SAVLNS+
Sbjct: 4712 LSAVLNSI 4719



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.320    0.116    0.262 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 258,959
Number of extensions: 176
Number of successful extensions: 20
Number of sequences better than 10000.0: 20
Number of HSP's gapped: 20
Number of HSP's successfully gapped: 20
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)