Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_444256                                                              19     665
NP_444254                                                              19     665
NP_444253                                                              19     665
NP_444255                                                              19     665
NP_444260                                                              19     665
NP_444259                                                              19     665
XP_005247551                                                           19     665
XP_005247550                                                           19     665
XP_006713712                                                           19     665
XP_005247549                                                           19     665
NP_775924                                                              16    5631
NP_001129145                                                           16    5631
NP_001244220                                                           16    5631
NP_115980                                                              16    5631
NP_071769                                                              16    5631
XP_006724627                                                           16    5631
XP_005272741                                                           16    5631
XP_005272739                                                           16    5631
XP_005272738                                                           16    5631
XP_005272737                                                           16    5631
XP_006712135                                                           16    5631
XP_006712134                                                           16    5631
XP_006712133                                                           16    5631
NP_004095                                                              15    9605
NP_775871                                                              15    9605
XP_006723528                                                           15    9605
XP_006723527                                                           15    9605
NP_001004478                                                           15    9605
>NP_444256
          Length = 1794

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    MVMISGLSG 9
            M MISGLSG
Sbjct: 1641 MAMISGLSG 1649
>NP_444254
          Length = 1845

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    MVMISGLSG 9
            M MISGLSG
Sbjct: 1692 MAMISGLSG 1700
>NP_444253
          Length = 1914

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    MVMISGLSG 9
            M MISGLSG
Sbjct: 1761 MAMISGLSG 1769
>NP_444255
          Length = 1863

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    MVMISGLSG 9
            M MISGLSG
Sbjct: 1710 MAMISGLSG 1718
>NP_444260
          Length = 154

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1 MVMISGLSG 9
         M MISGLSG
Sbjct: 1 MAMISGLSG 9
>NP_444259
          Length = 153

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1 MVMISGLSG 9
         M MISGLSG
Sbjct: 1 MAMISGLSG 9
>XP_005247551
          Length = 1855

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    MVMISGLSG 9
            M MISGLSG
Sbjct: 1702 MAMISGLSG 1710
>XP_005247550
          Length = 1856

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    MVMISGLSG 9
            M MISGLSG
Sbjct: 1703 MAMISGLSG 1711
>XP_006713712
          Length = 1923

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    MVMISGLSG 9
            M MISGLSG
Sbjct: 1771 MAMISGLSG 1779
>XP_005247549
          Length = 1924

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    MVMISGLSG 9
            M MISGLSG
Sbjct: 1771 MAMISGLSG 1779
>NP_775924
          Length = 645

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   MVMISGLSG 9
           M+M SGL G
Sbjct: 434 MMMFSGLRG 442
>NP_001129145
          Length = 583

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MVMISGLSG 9
           M +ISGL+G
Sbjct: 485 MYIISGLTG 493
>NP_001244220
          Length = 726

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   MVMISGLSG 9
           M+M SGL G
Sbjct: 483 MMMFSGLRG 491
>NP_115980
          Length = 725

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   MVMISGLSG 9
           M+M SGL G
Sbjct: 482 MMMFSGLRG 490
>NP_071769
          Length = 579

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MVMISGLSG 9
           M +ISGL+G
Sbjct: 485 MYIISGLTG 493
>XP_006724627
          Length = 706

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   MVMISGLSG 9
           M+M SGL G
Sbjct: 483 MMMFSGLRG 491
>XP_005272741
          Length = 757

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   MVMISGLSG 9
           M+M SGL G
Sbjct: 483 MMMFSGLRG 491
>XP_005272739
          Length = 726

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   MVMISGLSG 9
           M+M SGL G
Sbjct: 483 MMMFSGLRG 491
>XP_005272738
          Length = 726

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   MVMISGLSG 9
           M+M SGL G
Sbjct: 483 MMMFSGLRG 491
>XP_005272737
          Length = 737

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   MVMISGLSG 9
           M+M SGL G
Sbjct: 483 MMMFSGLRG 491
>XP_006712135
          Length = 529

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MVMISGLSG 9
           M +ISGL+G
Sbjct: 431 MYIISGLTG 439
>XP_006712134
          Length = 565

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MVMISGLSG 9
           M +ISGL+G
Sbjct: 467 MYIISGLTG 475
>XP_006712133
          Length = 578

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MVMISGLSG 9
           M +ISGL+G
Sbjct: 480 MYIISGLTG 488
>NP_004095
          Length = 2511

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  VMISGLSG 9
          V+I+G+SG
Sbjct: 4  VVIAGMSG 11
>NP_775871
          Length = 8384

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    ISGLSG 9
            ISGLSG
Sbjct: 5212 ISGLSG 5217
>XP_006723528
          Length = 649

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  MISGLSG 9
          ++SGLSG
Sbjct: 24 LLSGLSG 30
>XP_006723527
          Length = 661

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  MISGLSG 9
          ++SGLSG
Sbjct: 24 LLSGLSG 30
>NP_001004478
          Length = 313

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  MISGLSG 9
          M+SGL+G
Sbjct: 81 MLSGLAG 87
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.324    0.138    0.359 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 395,904
Number of extensions: 443
Number of successful extensions: 28
Number of sequences better than 10000.0: 28
Number of HSP's gapped: 28
Number of HSP's successfully gapped: 28
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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