Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006197                                                              17    2528
XP_006714104                                                           17    2528
XP_005265800                                                           17    2528
XP_006714103                                                           17    2528
XP_006714102                                                           17    2528
NP_064522                                                              16    7355
XP_005272685                                                           16    7355
NP_001032961                                                           15    9605
NP_057400                                                              15    9605
XP_006712656                                                           15    9605
XP_005264025                                                           15    9605
XP_006712655                                                           15    9605

>NP_006197
          Length = 1089

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  VLEVSSAS 9
          VLEVSSAS
Sbjct: 84 VLEVSSAS 91



>XP_006714104
          Length = 832

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   VLEVSSAS 9
           VLEVSSAS
Sbjct: 109 VLEVSSAS 116



>XP_005265800
          Length = 1089

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  VLEVSSAS 9
          VLEVSSAS
Sbjct: 84 VLEVSSAS 91



>XP_006714103
          Length = 1111

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   VLEVSSAS 9
           VLEVSSAS
Sbjct: 106 VLEVSSAS 113



>XP_006714102
          Length = 1114

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   VLEVSSAS 9
           VLEVSSAS
Sbjct: 109 VLEVSSAS 116



>NP_064522
          Length = 841

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   LEVSSAS 9
           LEVSSAS
Sbjct: 732 LEVSSAS 738



>XP_005272685
          Length = 815

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   LEVSSAS 9
           LEVSSAS
Sbjct: 706 LEVSSAS 712



>NP_001032961
          Length = 1250

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LEVSSAS 9
           LE+SSAS
Sbjct: 878 LEISSAS 884



>NP_057400
          Length = 1251

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LEVSSAS 9
           LE+SSAS
Sbjct: 879 LEISSAS 885



>XP_006712656
          Length = 761

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LEVSSAS 9
           LE+SSAS
Sbjct: 389 LEISSAS 395



>XP_005264025
          Length = 1181

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LEVSSAS 9
           LE+SSAS
Sbjct: 809 LEISSAS 815



>XP_006712655
          Length = 1251

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LEVSSAS 9
           LE+SSAS
Sbjct: 879 LEISSAS 885



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.299    0.106    0.217 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 231,166
Number of extensions: 168
Number of successful extensions: 12
Number of sequences better than 10000.0: 12
Number of HSP's gapped: 12
Number of HSP's successfully gapped: 12
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)