Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              18    1482
NP_671714                                                              18    1482
XP_006715890                                                           18    1482
XP_005250160                                                           18    1482
XP_005250159                                                           18    1482
NP_006874                                                              16    5631
NP_000442                                                              16    5631
NP_005520                                                              16    7355
NP_001186692                                                           16    7355
NP_001159635                                                           16    7355
NP_001159632                                                           16    7355
NP_001159634                                                           16    7355
NP_001159633                                                           16    7355
NP_002837                                                              16    7355
NP_001186693                                                           16    7355
XP_006716111                                                           16    7355
XP_006716110                                                           16    7355
XP_005250542                                                           16    7355
XP_006716109                                                           16    7355
XP_006710661                                                           16    7355
XP_005245920                                                           16    7355
XP_006710660                                                           16    7355
XP_006710659                                                           16    7355
XP_006710658                                                           16    7355
XP_006710657                                                           16    7355
NP_060120                                                              16    7355
NP_001135760                                                           15    9605
NP_006058                                                              15    9605
XP_006726587                                                           15    9605
XP_006710006                                                           15    9605

>NP_005742
          Length = 3907

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    MTSRVAEGS 9
            MTSR AEGS
Sbjct: 1026 MTSRGAEGS 1034



>NP_671714
          Length = 3899

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    MTSRVAEGS 9
            MTSR AEGS
Sbjct: 1026 MTSRGAEGS 1034



>XP_006715890
          Length = 3860

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    MTSRVAEGS 9
            MTSR AEGS
Sbjct: 1026 MTSRGAEGS 1034



>XP_005250160
          Length = 3939

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    MTSRVAEGS 9
            MTSR AEGS
Sbjct: 1026 MTSRGAEGS 1034



>XP_005250159
          Length = 3952

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    MTSRVAEGS 9
            MTSR AEGS
Sbjct: 1026 MTSRGAEGS 1034



>NP_006874
          Length = 225

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  TSRVAEG 8
          T+RVAEG
Sbjct: 87 TARVAEG 93



>NP_000442
          Length = 292

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  TSRVAEG 8
          T+RVAEG
Sbjct: 87 TARVAEG 93



>NP_005520
          Length = 4391

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    TSRVAEG 8
            +SRVAEG
Sbjct: 2835 SSRVAEG 2841



>NP_001186692
          Length = 950

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SRVAEGS 9
           SRV+EGS
Sbjct: 220 SRVSEGS 226



>NP_001159635
          Length = 1090

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    MTSRVAEGS 9
            MTS  AEG+
Sbjct: 1079 MTSTTAEGA 1087



>NP_001159632
          Length = 1374

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    MTSRVAEGS 9
            MTS  AEG+
Sbjct: 1363 MTSTTAEGA 1371



>NP_001159634
          Length = 1253

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    MTSRVAEGS 9
            MTS  AEG+
Sbjct: 1242 MTSTTAEGA 1250



>NP_001159633
          Length = 1296

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    MTSRVAEGS 9
            MTS  AEG+
Sbjct: 1285 MTSTTAEGA 1293



>NP_002837
          Length = 979

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SRVAEGS 9
           SRV+EGS
Sbjct: 220 SRVSEGS 226



>NP_001186693
          Length = 889

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SRVAEGS 9
           SRV+EGS
Sbjct: 130 SRVSEGS 136



>XP_006716111
          Length = 701

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   MTSRVAEGS 9
           MTS  AEG+
Sbjct: 690 MTSTTAEGA 698



>XP_006716110
          Length = 1141

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    MTSRVAEGS 9
            MTS  AEG+
Sbjct: 1130 MTSTTAEGA 1138



>XP_005250542
          Length = 1357

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    MTSRVAEGS 9
            MTS  AEG+
Sbjct: 1346 MTSTTAEGA 1354



>XP_006716109
          Length = 1365

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    MTSRVAEGS 9
            MTS  AEG+
Sbjct: 1354 MTSTTAEGA 1362



>XP_006710661
          Length = 4057

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    TSRVAEG 8
            +SRVAEG
Sbjct: 3018 SSRVAEG 3024



>XP_005245920
          Length = 4392

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    TSRVAEG 8
            +SRVAEG
Sbjct: 2836 SSRVAEG 2842



>XP_006710660
          Length = 4397

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    TSRVAEG 8
            +SRVAEG
Sbjct: 2835 SSRVAEG 2841



>XP_006710659
          Length = 4556

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    TSRVAEG 8
            +SRVAEG
Sbjct: 2994 SSRVAEG 3000



>XP_006710658
          Length = 4563

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    TSRVAEG 8
            +SRVAEG
Sbjct: 3001 SSRVAEG 3007



>XP_006710657
          Length = 4579

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    TSRVAEG 8
            +SRVAEG
Sbjct: 3017 SSRVAEG 3023



>NP_060120
          Length = 1098

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    MTSRVAEGS 9
            MTS  AEG+
Sbjct: 1087 MTSTTAEGA 1095



>NP_001135760
          Length = 126

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   MTSRVAEG 8
           + SR+AEG
Sbjct: 111 VASRIAEG 118



>NP_006058
          Length = 210

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   MTSRVAEG 8
           + SR+AEG
Sbjct: 111 VASRIAEG 118



>XP_006726587
          Length = 218

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   RVAEGS 9
           RVAEGS
Sbjct: 208 RVAEGS 213



>XP_006710006
          Length = 218

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   RVAEGS 9
           RVAEGS
Sbjct: 208 RVAEGS 213



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.300    0.109    0.259 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 378,336
Number of extensions: 391
Number of successful extensions: 30
Number of sequences better than 10000.0: 30
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)