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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              20     390
XP_005262424                                                           20     390
XP_005258838                                                           17    4312
NP_775904                                                              17    4312
NP_878908                                                              16    5631
NP_982272                                                              16    5631
NP_055994                                                              16    5631
NP_001138586                                                           16    5631
NP_001177387                                                           16    5631
NP_001180465                                                           16    5631
NP_001271266                                                           16    5631
XP_006726924                                                           16    5631
NP_001186054                                                           16    7355
NP_003644                                                              16    7355
XP_005256899                                                           16    7355
XP_005256898                                                           16    7355
XP_005256897                                                           16    7355
XP_005256896                                                           16    7355
XP_005256895                                                           16    7355
XP_005256894                                                           16    7355
NP_006454                                                              15    9605
NP_998787                                                              15    9605
NP_964010                                                              15    9605
NP_061859                                                              15    9605
XP_006722961                                                           15    9605
XP_006713954                                                           15    9605
XP_006713953                                                           15    9605
XP_006713952                                                           15    9605
XP_005248031                                                           15    9605
XP_006713951                                                           15    9605
XP_005264148                                                           15    9605
XP_005264145                                                           15    9605
XP_006711984                                                           15    9605
XP_006711983                                                           15    9605

>NP_722576
          Length = 3080

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    MTLTHVMLQ 9
            M LTHVMLQ
Sbjct: 2508 MNLTHVMLQ 2516



>XP_005262424
          Length = 2875

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    MTLTHVMLQ 9
            M LTHVMLQ
Sbjct: 2303 MNLTHVMLQ 2311



>XP_005258838
          Length = 563

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   TLTHVML 8
           TLTHV+L
Sbjct: 302 TLTHVVL 308



>NP_775904
          Length = 493

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   TLTHVML 8
           TLTHV+L
Sbjct: 288 TLTHVVL 294



>NP_878908
          Length = 465

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   MTLTHVML 8
           +T THVML
Sbjct: 196 VTKTHVML 203



>NP_982272
          Length = 2099

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    MTLTHVMLQ 9
            +TLTH  LQ
Sbjct: 1768 LTLTHSKLQ 1776



>NP_055994
          Length = 1297

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MTLTHVML 8
           MTL+HV++
Sbjct: 334 MTLSHVLV 341



>NP_001138586
          Length = 1236

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MTLTHVML 8
           MTL+HV++
Sbjct: 273 MTLSHVLV 280



>NP_001177387
          Length = 1999

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    MTLTHVMLQ 9
            +TLTH  LQ
Sbjct: 1668 LTLTHSKLQ 1676



>NP_001180465
          Length = 2031

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    MTLTHVMLQ 9
            +TLTH  LQ
Sbjct: 1700 LTLTHSKLQ 1708



>NP_001271266
          Length = 1197

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MTLTHVML 8
           MTL+HV++
Sbjct: 234 MTLSHVLV 241



>XP_006726924
          Length = 451

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   MTLTHVML 8
           +T THVML
Sbjct: 182 VTKTHVML 189



>NP_001186054
          Length = 403

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   MTLTHVMLQ 9
           M ++H+ML+
Sbjct: 194 MAVSHIMLE 202



>NP_003644
          Length = 423

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   MTLTHVMLQ 9
           M ++H+ML+
Sbjct: 214 MAVSHIMLE 222



>XP_005256899
          Length = 293

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  MTLTHVMLQ 9
          M ++H+ML+
Sbjct: 84 MAVSHIMLE 92



>XP_005256898
          Length = 293

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  MTLTHVMLQ 9
          M ++H+ML+
Sbjct: 84 MAVSHIMLE 92



>XP_005256897
          Length = 293

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  MTLTHVMLQ 9
          M ++H+ML+
Sbjct: 84 MAVSHIMLE 92



>XP_005256896
          Length = 356

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   MTLTHVMLQ 9
           M ++H+ML+
Sbjct: 147 MAVSHIMLE 155



>XP_005256895
          Length = 365

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   MTLTHVMLQ 9
           M ++H+ML+
Sbjct: 214 MAVSHIMLE 222



>XP_005256894
          Length = 403

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   MTLTHVMLQ 9
           M ++H+ML+
Sbjct: 194 MAVSHIMLE 202



>NP_006454
          Length = 424

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   TLTHVML 8
           T+THV++
Sbjct: 295 TITHVLI 301



>NP_998787
          Length = 424

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   TLTHVML 8
           T+THV++
Sbjct: 295 TITHVLI 301



>NP_964010
          Length = 424

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   TLTHVML 8
           T+THV++
Sbjct: 295 TITHVLI 301



>NP_061859
          Length = 1336

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   THVMLQ 9
           THV+LQ
Sbjct: 946 THVLLQ 951



>XP_006722961
          Length = 214

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  TLTHVMLQ 9
          T+TH +LQ
Sbjct: 17 TMTHRLLQ 24



>XP_006713954
          Length = 1043

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   THVMLQ 9
           THV+LQ
Sbjct: 653 THVLLQ 658



>XP_006713953
          Length = 1043

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   THVMLQ 9
           THV+LQ
Sbjct: 653 THVLLQ 658



>XP_006713952
          Length = 1165

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   THVMLQ 9
           THV+LQ
Sbjct: 775 THVLLQ 780



>XP_005248031
          Length = 1260

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   THVMLQ 9
           THV+LQ
Sbjct: 870 THVLLQ 875



>XP_006713951
          Length = 1309

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   THVMLQ 9
           THV+LQ
Sbjct: 919 THVLLQ 924



>XP_005264148
          Length = 289

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   TLTHVML 8
           T+THV++
Sbjct: 160 TITHVLI 166



>XP_005264145
          Length = 424

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   TLTHVML 8
           T+THV++
Sbjct: 295 TITHVLI 301



>XP_006711984
          Length = 457

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   TLTHVML 8
           T+THV++
Sbjct: 342 TITHVLI 348



>XP_006711983
          Length = 471

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   TLTHVML 8
           T+THV++
Sbjct: 342 TITHVLI 348



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.338    0.130    0.360 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 506,986
Number of extensions: 611
Number of successful extensions: 34
Number of sequences better than 10000.0: 34
Number of HSP's gapped: 34
Number of HSP's successfully gapped: 34
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.6 bits)
S2: 28 (15.4 bits)