Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              19     665
XP_005262424                                                           19     665
NP_689777                                                              17    3301
NP_060360                                                              17    3301
NP_056058                                                              17    3301
XP_006716574                                                           17    3301
XP_006716573                                                           17    3301
XP_005250858                                                           17    3301
XP_005250857                                                           17    3301
NP_001714                                                              15    9605
NP_149020                                                              15    9605
NP_149438                                                              15    9605
NP_001014765                                                           15    9605
XP_006724673                                                           15    9605
XP_005249383                                                           15    9605
XP_006711109                                                           15    9605
XP_006711108                                                           15    9605
XP_005263595                                                           15    9605
XP_006711107                                                           15    9605
NP_061979                                                              15    9605
NP_001070710                                                           15    9605
NP_663786                                                              15    9605
>NP_722576
          Length = 3080

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   MTIQEKST 8
           MTIQEKST
Sbjct: 234 MTIQEKST 241
>XP_005262424
          Length = 2875

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1  MTIQEKST 8
          MTIQEKST
Sbjct: 29 MTIQEKST 36
>NP_689777
          Length = 3997

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   TIQEKST 8
           T+QEKST
Sbjct: 568 TVQEKST 574
>NP_060360
          Length = 4022

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   TIQEKST 8
           T+QEKST
Sbjct: 568 TVQEKST 574
>NP_056058
          Length = 863

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   TIQEKST 8
           T+QEKST
Sbjct: 568 TVQEKST 574
>XP_006716574
          Length = 968

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   TIQEKST 8
           T+QEKST
Sbjct: 568 TVQEKST 574
>XP_006716573
          Length = 1776

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   TIQEKST 8
           T+QEKST
Sbjct: 568 TVQEKST 574
>XP_005250858
          Length = 4022

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   TIQEKST 8
           T+QEKST
Sbjct: 568 TVQEKST 574
>XP_005250857
          Length = 4022

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   TIQEKST 8
           T+QEKST
Sbjct: 568 TVQEKST 574
>NP_001714
          Length = 2649

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    IQEKSTA 9
            IQ+KSTA
Sbjct: 1867 IQKKSTA 1873
>NP_149020
          Length = 1395

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   MTIQEKST 8
           + +QEKST
Sbjct: 711 LALQEKST 718
>NP_149438
          Length = 255

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TIQEKSTA 9
           TIQ+KS A
Sbjct: 197 TIQQKSDA 204
>NP_001014765
          Length = 255

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TIQEKSTA 9
           TIQ+KS A
Sbjct: 197 TIQQKSDA 204
>XP_006724673
          Length = 1396

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   MTIQEKST 8
           + +QEKST
Sbjct: 711 LALQEKST 718
>XP_005249383
          Length = 3186

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    IQEKSTA 9
            IQ+KSTA
Sbjct: 2404 IQKKSTA 2410
>XP_006711109
          Length = 255

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TIQEKSTA 9
           TIQ+KS A
Sbjct: 197 TIQQKSDA 204
>XP_006711108
          Length = 255

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TIQEKSTA 9
           TIQ+KS A
Sbjct: 197 TIQQKSDA 204
>XP_005263595
          Length = 255

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TIQEKSTA 9
           TIQ+KS A
Sbjct: 197 TIQQKSDA 204
>XP_006711107
          Length = 255

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TIQEKSTA 9
           TIQ+KS A
Sbjct: 197 TIQQKSDA 204
>NP_061979
          Length = 350

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MTIQEKS 7
           MT++EKS
Sbjct: 212 MTLEEKS 218
>NP_001070710
          Length = 511

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  TIQEKSTA 9
          T+QEK+ A
Sbjct: 3  TVQEKAAA 10
>NP_663786
          Length = 358

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MTIQEKS 7
           MT++EKS
Sbjct: 212 MTLEEKS 218
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.297    0.102    0.225 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 429,253
Number of extensions: 163
Number of successful extensions: 22
Number of sequences better than 10000.0: 22
Number of HSP's gapped: 22
Number of HSP's successfully gapped: 22
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)

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