Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_789765                                                              20     390
NP_001123914                                                           20     390
NP_005334                                                              20     390
NP_004976                                                              20     390
NP_203524                                                              20     390
NP_002515                                                              20     390
XP_006725199                                                           20     390
XP_006719132                                                           20     390
XP_006718280                                                           20     390
NP_006266                                                              17    2528
NP_001983                                                              17    2528
NP_005617                                                              17    2528
NP_001136408                                                           17    3301
NP_631918                                                              17    3301
NP_001238851                                                           17    4312
NP_001238847                                                           17    4312
NP_001238846                                                           17    4312
NP_056461                                                              17    4312
NP_001238850                                                           17    4312
NP_001010942                                                           17    4312
XP_006710866                                                           17    4312
NP_001010935                                                           17    4312
NP_002875                                                              17    4312
NP_060109                                                              16    5631
NP_036382                                                              16    7355
NP_055621                                                              15    9605
NP_001129471                                                           15    9605
NP_056098                                                              15    9605
NP_076407                                                              15    9605
>NP_789765
          Length = 170

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1 MTEYRLVVV 9
         MTEY+LVVV
Sbjct: 1 MTEYKLVVV 9
>NP_001123914
          Length = 189

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1 MTEYRLVVV 9
         MTEY+LVVV
Sbjct: 1 MTEYKLVVV 9
>NP_005334
          Length = 189

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1 MTEYRLVVV 9
         MTEY+LVVV
Sbjct: 1 MTEYKLVVV 9
>NP_004976
          Length = 188

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1 MTEYRLVVV 9
         MTEY+LVVV
Sbjct: 1 MTEYKLVVV 9
>NP_203524
          Length = 189

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1 MTEYRLVVV 9
         MTEY+LVVV
Sbjct: 1 MTEYKLVVV 9
>NP_002515
          Length = 189

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1 MTEYRLVVV 9
         MTEY+LVVV
Sbjct: 1 MTEYKLVVV 9
>XP_006725199
          Length = 170

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1 MTEYRLVVV 9
         MTEY+LVVV
Sbjct: 1 MTEYKLVVV 9
>XP_006719132
          Length = 189

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1 MTEYRLVVV 9
         MTEY+LVVV
Sbjct: 1 MTEYKLVVV 9
>XP_006718280
          Length = 170

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1 MTEYRLVVV 9
         MTEY+LVVV
Sbjct: 1 MTEYKLVVV 9
>NP_006266
          Length = 344

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   TEYRLVV 8
           TEYRL+V
Sbjct: 108 TEYRLIV 114
>NP_001983
          Length = 425

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  MTEYRLVVV 9
          +TEYRLV +
Sbjct: 66 LTEYRLVSI 74
>NP_005617
          Length = 494

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   TEYRLVV 8
           TEYRL+V
Sbjct: 102 TEYRLIV 108
>NP_001136408
          Length = 543

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   EYRLVVV 9
           EYRLV+V
Sbjct: 305 EYRLVIV 311
>NP_631918
          Length = 672

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   EYRLVVV 9
           EYRLV+V
Sbjct: 434 EYRLVIV 440
>NP_001238851
          Length = 137

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1 MTEYRLVVV 9
         M EY+LVV+
Sbjct: 1 MREYKLVVL 9
>NP_001238847
          Length = 142

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1 MTEYRLVVV 9
         M EY+LVV+
Sbjct: 1 MREYKLVVL 9
>NP_001238846
          Length = 142

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1 MTEYRLVVV 9
         M EY+LVV+
Sbjct: 1 MREYKLVVL 9
>NP_056461
          Length = 184

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1 MTEYRLVVV 9
         M EY+LVV+
Sbjct: 1 MREYKLVVL 9
>NP_001238850
          Length = 165

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1 MTEYRLVVV 9
         M EY+LVV+
Sbjct: 1 MREYKLVVL 9
>NP_001010942
          Length = 184

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1 MTEYRLVVV 9
         M EY+LVV+
Sbjct: 1 MREYKLVVL 9
>XP_006710866
          Length = 184

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1 MTEYRLVVV 9
         M EY+LVV+
Sbjct: 1 MREYKLVVL 9
>NP_001010935
          Length = 184

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1 MTEYRLVVV 9
         M EY+LVV+
Sbjct: 1 MREYKLVVL 9
>NP_002875
          Length = 184

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1 MTEYRLVVV 9
         M EY+LVV+
Sbjct: 1 MREYKLVVL 9
>NP_060109
          Length = 2916

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    MTEYRLVV 8
            MTEY L++
Sbjct: 1958 MTEYELLI 1965
>NP_036382
          Length = 204

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  EYRLVVV 9
          +YRLVVV
Sbjct: 14 KYRLVVV 20
>NP_055621
          Length = 1516

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   MTEYRL 6
           +TEYRL
Sbjct: 843 LTEYRL 848
>NP_001129471
          Length = 1516

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   MTEYRL 6
           +TEYRL
Sbjct: 843 LTEYRL 848
>NP_056098
          Length = 675

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  MTEYRLVV 8
          +TEY LV+
Sbjct: 92 LTEYPLVI 99
>NP_076407
          Length = 309

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   MTEYRLVV 8
           MT+Y+L V
Sbjct: 260 MTKYKLAV 267
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.336    0.143    0.372 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 425,286
Number of extensions: 488
Number of successful extensions: 29
Number of sequences better than 10000.0: 29
Number of HSP's gapped: 29
Number of HSP's successfully gapped: 29
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)

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