Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              19     665
NP_671714                                                              19     665
XP_006715890                                                           19     665
XP_005250160                                                           19     665
XP_005250159                                                           19     665
NP_001186217                                                           17    2528
NP_001186216                                                           17    2528
NP_001186215                                                           17    2528
NP_001186214                                                           17    2528
NP_001008489                                                           17    2528
NP_758442                                                              17    3301
XP_005252703                                                           17    3301
XP_005252702                                                           17    3301
NP_036575                                                              17    3301
NP_001240784                                                           17    3301
NP_001276089                                                           17    4312
NP_005922                                                              17    4312
NP_055907                                                              17    4312
XP_006726158                                                           17    4312
XP_006726065                                                           17    4312
XP_005255242                                                           17    4312
XP_005255241                                                           17    4312
XP_005255240                                                           17    4312
XP_005255239                                                           17    4312
NP_000238                                                              16    5631
NP_001165132                                                           16    5631
NP_001123291                                                           16    5631
NP_015565                                                              16    5631
NP_001165131                                                           16    5631
NP_000389                                                              16    5631
NP_078796                                                              16    7355
NP_001276081                                                           16    7355
NP_001170990                                                           16    7355
NP_001276082                                                           16    7355
XP_005271964                                                           16    7355
XP_006714621                                                           16    7355
NP_078850                                                              16    7355
NP_689761                                                              16    7355
NP_060579                                                              15    9605
NP_056094                                                              15    9605
XP_006719364                                                           15    9605
XP_006719363                                                           15    9605
>NP_005742
          Length = 3907

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   MSQNIETMQ 9
           MSQ IETMQ
Sbjct: 674 MSQKIETMQ 682
>NP_671714
          Length = 3899

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   MSQNIETMQ 9
           MSQ IETMQ
Sbjct: 674 MSQKIETMQ 682
>XP_006715890
          Length = 3860

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   MSQNIETMQ 9
           MSQ IETMQ
Sbjct: 674 MSQKIETMQ 682
>XP_005250160
          Length = 3939

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   MSQNIETMQ 9
           MSQ IETMQ
Sbjct: 674 MSQKIETMQ 682
>XP_005250159
          Length = 3952

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   MSQNIETMQ 9
           MSQ IETMQ
Sbjct: 674 MSQKIETMQ 682
>NP_001186217
          Length = 241

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MSQNIETMQ 9
           MSQN+E M+
Sbjct: 211 MSQNLEPME 219
>NP_001186216
          Length = 241

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MSQNIETMQ 9
           MSQN+E M+
Sbjct: 211 MSQNLEPME 219
>NP_001186215
          Length = 241

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MSQNIETMQ 9
           MSQN+E M+
Sbjct: 211 MSQNLEPME 219
>NP_001186214
          Length = 241

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MSQNIETMQ 9
           MSQN+E M+
Sbjct: 211 MSQNLEPME 219
>NP_001008489
          Length = 241

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MSQNIETMQ 9
           MSQN+E M+
Sbjct: 211 MSQNLEPME 219
>NP_758442
          Length = 458

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  QNIETMQ 9
          QNIET+Q
Sbjct: 32 QNIETLQ 38
>XP_005252703
          Length = 451

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  QNIETMQ 9
          QNIET+Q
Sbjct: 32 QNIETLQ 38
>XP_005252702
          Length = 506

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  QNIETMQ 9
          QNIET+Q
Sbjct: 32 QNIETLQ 38
>NP_036575
          Length = 509

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  QNIETMQ 9
          QNIET+Q
Sbjct: 32 QNIETLQ 38
>NP_001240784
          Length = 484

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  QNIETMQ 9
          QNIET+Q
Sbjct: 10 QNIETLQ 16
>NP_001276089
          Length = 351

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MSQNIETMQ 9
           +SQN+ET +
Sbjct: 109 LSQNLETQE 117
>NP_005922
          Length = 383

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MSQNIETMQ 9
           +SQN+ET +
Sbjct: 141 LSQNLETQE 149
>NP_055907
          Length = 3661

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    MSQNIET 7
            M+QNIET
Sbjct: 2375 MTQNIET 2381
>XP_006726158
          Length = 351

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MSQNIETMQ 9
           +SQN+ET +
Sbjct: 109 LSQNLETQE 117
>XP_006726065
          Length = 351

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MSQNIETMQ 9
           +SQN+ET +
Sbjct: 109 LSQNLETQE 117
>XP_005255242
          Length = 3609

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    MSQNIET 7
            M+QNIET
Sbjct: 2323 MTQNIET 2329
>XP_005255241
          Length = 3637

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    MSQNIET 7
            M+QNIET
Sbjct: 2351 MTQNIET 2357
>XP_005255240
          Length = 3663

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    MSQNIET 7
            M+QNIET
Sbjct: 2377 MTQNIET 2383
>XP_005255239
          Length = 3689

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    MSQNIET 7
            M+QNIET
Sbjct: 2403 MTQNIET 2409
>NP_000238
          Length = 383

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MSQNIETMQ 9
           +SQN+ET +
Sbjct: 141 LSQNLETKE 149
>NP_001165132
          Length = 278

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MSQNIETMQ 9
           MSQ +E+MQ
Sbjct: 104 MSQYLESMQ 112
>NP_001123291
          Length = 278

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MSQNIETMQ 9
           MSQ +E+MQ
Sbjct: 104 MSQYLESMQ 112
>NP_015565
          Length = 278

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MSQNIETMQ 9
           MSQ +E+MQ
Sbjct: 104 MSQYLESMQ 112
>NP_001165131
          Length = 334

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MSQNIETMQ 9
           MSQ +E+MQ
Sbjct: 160 MSQYLESMQ 168
>NP_000389
          Length = 301

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MSQNIETMQ 9
           MSQ +E+MQ
Sbjct: 127 MSQYLESMQ 135
>NP_078796
          Length = 291

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SQNIETMQ 9
           +QN+ET Q
Sbjct: 252 NQNVETKQ 259
>NP_001276081
          Length = 235

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  MSQNIET 7
          +SQN+ET
Sbjct: 44 LSQNLET 50
>NP_001170990
          Length = 332

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MSQNIET 7
           +SQN+ET
Sbjct: 141 LSQNLET 147
>NP_001276082
          Length = 235

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  MSQNIET 7
          +SQN+ET
Sbjct: 44 LSQNLET 50
>XP_005271964
          Length = 232

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MSQNIETMQ 9
           +SQNIE ++
Sbjct: 174 LSQNIEILE 182
>XP_006714621
          Length = 368

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  MSQNIETMQ 9
          +SQNIE ++
Sbjct: 90 LSQNIEILE 98
>NP_078850
          Length = 568

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MSQNIETMQ 9
           +SQN+E +Q
Sbjct: 397 LSQNVEGIQ 405
>NP_689761
          Length = 452

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MSQNIETMQ 9
           +SQNIE ++
Sbjct: 174 LSQNIEILE 182
>NP_060579
          Length = 582

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SQNIETMQ 9
           SQN ET++
Sbjct: 431 SQNTETLE 438
>NP_056094
          Length = 761

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   MSQNIETMQ 9
           +SQ+++T+Q
Sbjct: 238 LSQHLDTLQ 246
>XP_006719364
          Length = 924

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   MSQNIETMQ 9
           +SQ+++T+Q
Sbjct: 401 LSQHLDTLQ 409
>XP_006719363
          Length = 944

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   MSQNIETMQ 9
           +SQ+++T+Q
Sbjct: 401 LSQHLDTLQ 409
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.305    0.108    0.249 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 350,611
Number of extensions: 494
Number of successful extensions: 42
Number of sequences better than 10000.0: 42
Number of HSP's gapped: 42
Number of HSP's successfully gapped: 42
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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