Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001185951                                                           17    3301
NP_055526                                                              17    3301
XP_006721168                                                           17    3301
XP_005255778                                                           17    3301
XP_005255776                                                           17    3301
NP_002542                                                              17    4312
NP_056372                                                              17    4312
NP_036505                                                              17    4312
NP_001166246                                                           16    7355
NP_113614                                                              16    7355
NP_690004                                                              16    7355
NP_001172025                                                           16    7355
NP_001172024                                                           16    7355
NP_940931                                                              16    7355
XP_006721943                                                           16    7355
XP_006721942                                                           16    7355
NP_005742                                                              15    9605
NP_671714                                                              15    9605
NP_079143                                                              15    9605
NP_653323                                                              15    9605
XP_005268211                                                           15    9605
XP_006715890                                                           15    9605
XP_005250160                                                           15    9605
XP_005250159                                                           15    9605
XP_005248435                                                           15    9605
XP_006714564                                                           15    9605
XP_006714563                                                           15    9605
XP_005248434                                                           15    9605
XP_005248433                                                           15    9605
NP_001092872                                                           15    9605
>NP_001185951
          Length = 1012

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MSLQKMMKR 9
           MSLQ +MK+
Sbjct: 226 MSLQNLMKK 234
>NP_055526
          Length = 991

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MSLQKMMKR 9
           MSLQ +MK+
Sbjct: 226 MSLQNLMKK 234
>XP_006721168
          Length = 971

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MSLQKMMKR 9
           MSLQ +MK+
Sbjct: 226 MSLQNLMKK 234
>XP_005255778
          Length = 991

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MSLQKMMKR 9
           MSLQ +MK+
Sbjct: 226 MSLQNLMKK 234
>XP_005255776
          Length = 1012

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MSLQKMMKR 9
           MSLQ +MK+
Sbjct: 226 MSLQNLMKK 234
>NP_002542
          Length = 321

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1 MSLQKMMK 8
         MSLQK+M+
Sbjct: 1 MSLQKLME 8
>NP_056372
          Length = 1954

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    MSLQKMMKR 9
            M LQKM+K+
Sbjct: 1027 MLLQKMLKK 1035
>NP_036505
          Length = 321

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1 MSLQKMMK 8
         MSLQK+M+
Sbjct: 1 MSLQKLME 8
>NP_001166246
          Length = 627

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MSLQKMMKR 9
           +SLQK M+R
Sbjct: 492 ISLQKHMRR 500
>NP_113614
          Length = 150

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1  MSLQKMMKR 9
          MSL++MM++
Sbjct: 70 MSLKRMMEK 78
>NP_690004
          Length = 628

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MSLQKMMKR 9
           +SLQK M+R
Sbjct: 493 ISLQKHMRR 501
>NP_001172025
          Length = 102

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1  MSLQKMMKR 9
          MSL++MM++
Sbjct: 70 MSLKRMMEK 78
>NP_001172024
          Length = 176

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   MSLQKMMKR 9
           MSL++MM++
Sbjct: 96  MSLKRMMEK 104
>NP_940931
          Length = 1503

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    LQKMMK 8
            LQKMMK
Sbjct: 1284 LQKMMK 1289
>XP_006721943
          Length = 1456

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    LQKMMK 8
            LQKMMK
Sbjct: 1284 LQKMMK 1289
>XP_006721942
          Length = 1503

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    LQKMMK 8
            LQKMMK
Sbjct: 1284 LQKMMK 1289
>NP_005742
          Length = 3907

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    MSLQKMMKR 9
            ++LQK +KR
Sbjct: 3660 LTLQKSLKR 3668
>NP_671714
          Length = 3899

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    MSLQKMMKR 9
            ++LQK +KR
Sbjct: 3652 LTLQKSLKR 3660
>NP_079143
          Length = 1822

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   MSLQKMMKR 9
           +  QKMMK+
Sbjct: 229 LEAQKMMKK 237
>NP_653323
          Length = 514

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   MSLQKMMKR 9
           +  QKMMK+
Sbjct: 229 LEAQKMMKK 237
>XP_005268211
          Length = 580

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SLQKMMK 8
           SLQ MMK
Sbjct: 446 SLQSMMK 452
>XP_006715890
          Length = 3860

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    MSLQKMMKR 9
            ++LQK +KR
Sbjct: 3613 LTLQKSLKR 3621
>XP_005250160
          Length = 3939

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    MSLQKMMKR 9
            ++LQK +KR
Sbjct: 3692 LTLQKSLKR 3700
>XP_005250159
          Length = 3952

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    MSLQKMMKR 9
            ++LQK +KR
Sbjct: 3705 LTLQKSLKR 3713
>XP_005248435
          Length = 972

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   MSLQKMMKR 9
           +  QKMMK+
Sbjct: 229 LEAQKMMKK 237
>XP_006714564
          Length = 1537

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   MSLQKMMKR 9
           +  QKMMK+
Sbjct: 229 LEAQKMMKK 237
>XP_006714563
          Length = 1766

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   MSLQKMMKR 9
           +  QKMMK+
Sbjct: 172 LEAQKMMKK 180
>XP_005248434
          Length = 1818

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   MSLQKMMKR 9
           +  QKMMK+
Sbjct: 229 LEAQKMMKK 237
>XP_005248433
          Length = 1823

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   MSLQKMMKR 9
           +  QKMMK+
Sbjct: 229 LEAQKMMKK 237
>NP_001092872
          Length = 580

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SLQKMMK 8
           SLQ MMK
Sbjct: 446 SLQSMMK 452
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.333    0.123    0.293 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 303,085
Number of extensions: 454
Number of successful extensions: 30
Number of sequences better than 10000.0: 30
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.5 bits)
S2: 28 (15.4 bits)

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