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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              19     869
NP_001075109                                                           19     869
NP_055423                                                              16    7355
NP_001274739                                                           16    7355
NP_056169                                                              16    7355
NP_001032409                                                           16    7355
NP_001028200                                                           16    7355
XP_005272600                                                           16    7355
NP_001032410                                                           16    7355
NP_055191                                                              16    7355
NP_060596                                                              15    9605
NP_004696                                                              15    9605
NP_009166                                                              15    9605
XP_005248174                                                           15    9605
XP_005248173                                                           15    9605

>NP_008835
          Length = 4128

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   MSLFEVLLK 9
           +SLFEVLLK
Sbjct: 291 VSLFEVLLK 299



>NP_001075109
          Length = 4097

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   MSLFEVLLK 9
           +SLFEVLLK
Sbjct: 291 VSLFEVLLK 299



>NP_055423
          Length = 1253

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    MSLFEVLL 8
            +S+FEV+L
Sbjct: 1090 LSMFEVIL 1097



>NP_001274739
          Length = 1253

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    MSLFEVLL 8
            +S+FEV+L
Sbjct: 1090 LSMFEVIL 1097



>NP_056169
          Length = 1749

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    MSLFEVLLK 9
            MSL E LLK
Sbjct: 1401 MSLMEQLLK 1409



>NP_001032409
          Length = 1253

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    MSLFEVLL 8
            +S+FEV+L
Sbjct: 1089 LSMFEVIL 1096



>NP_001028200
          Length = 822

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MSLFEVLL 8
           +S+FEV+L
Sbjct: 659 LSMFEVIL 666



>XP_005272600
          Length = 1253

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    MSLFEVLL 8
            +S+FEV+L
Sbjct: 1090 LSMFEVIL 1097



>NP_001032410
          Length = 1253

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    MSLFEVLL 8
            +S+FEV+L
Sbjct: 1089 LSMFEVIL 1096



>NP_055191
          Length = 1253

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    MSLFEVLL 8
            +S+FEV+L
Sbjct: 1089 LSMFEVIL 1096



>NP_060596
          Length = 247

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   MSLFEVLL 8
           MS+F VLL
Sbjct: 110 MSIFPVLL 117



>NP_004696
          Length = 761

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   SLFEVLL 8
           SLFE+L+
Sbjct: 168 SLFEILI 174



>NP_009166
          Length = 962

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SLFEVLLK 9
           ++FEVLL+
Sbjct: 771 AIFEVLLR 778



>XP_005248174
          Length = 247

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   MSLFEVLL 8
           MS+F VLL
Sbjct: 110 MSIFPVLL 117



>XP_005248173
          Length = 247

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   MSLFEVLL 8
           MS+F VLL
Sbjct: 110 MSIFPVLL 117



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.337    0.148    0.363 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 338,861
Number of extensions: 545
Number of successful extensions: 15
Number of sequences better than 10000.0: 15
Number of HSP's gapped: 15
Number of HSP's successfully gapped: 15
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)