Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000213                                                              19     665
NP_001087241                                                           19     665
XP_005265799                                                           19     665
XP_005265798                                                           19     665
XP_005265797                                                           19     665
NP_001275896                                                           18    1935
NP_001275894                                                           18    1935
NP_077817                                                              18    1935
NP_001275893                                                           18    1935
XP_005247863                                                           18    1935
NP_001011544                                                           17    4312
NP_005357                                                              17    4312
NP_001258681                                                           17    4312
NP_005859                                                              16    5631
NP_056054                                                              16    7355
NP_057646                                                              16    7355
XP_005251905                                                           16    7355
XP_005251904                                                           16    7355
NP_071745                                                              16    7355
NP_001123612                                                           16    7355
NP_573403                                                              15    9605
NP_036604                                                              15    9605
NP_055085                                                              15    9605
NP_001229965                                                           15    9605
NP_001119807                                                           15    9605
NP_877496                                                              15    9605
XP_005260559                                                           15    9605
XP_005260558                                                           15    9605
XP_006723918                                                           15    9605
XP_005260557                                                           15    9605
XP_005260556                                                           15    9605
XP_005260555                                                           15    9605
XP_005260554                                                           15    9605
XP_006723917                                                           15    9605
XP_005260553                                                           15    9605
XP_006723055                                                           15    9605
XP_005258525                                                           15    9605
XP_006716601                                                           15    9605
>NP_000213
          Length = 976

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   MSKLKVLSY 9
           MS+LKVLSY
Sbjct: 638 MSELKVLSY 646
>NP_001087241
          Length = 972

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   MSKLKVLSY 9
           MS+LKVLSY
Sbjct: 634 MSELKVLSY 642
>XP_005265799
          Length = 973

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   MSKLKVLSY 9
           MS+LKVLSY
Sbjct: 635 MSELKVLSY 643
>XP_005265798
          Length = 976

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   MSKLKVLSY 9
           MS+LKVLSY
Sbjct: 639 MSELKVLSY 647
>XP_005265797
          Length = 977

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   MSKLKVLSY 9
           MS+LKVLSY
Sbjct: 639 MSELKVLSY 647
>NP_001275896
          Length = 445

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   MSKLKVLSY 9
           + KLKVLSY
Sbjct: 437 VKKLKVLSY 445
>NP_001275894
          Length = 577

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   MSKLKVLSY 9
           + KLKVLSY
Sbjct: 569 VKKLKVLSY 577
>NP_077817
          Length = 597

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   MSKLKVLSY 9
           + KLKVLSY
Sbjct: 589 VKKLKVLSY 597
>NP_001275893
          Length = 579

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   MSKLKVLSY 9
           + KLKVLSY
Sbjct: 571 VKKLKVLSY 579
>XP_005247863
          Length = 597

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   MSKLKVLSY 9
           + KLKVLSY
Sbjct: 589 VKKLKVLSY 597
>NP_001011544
          Length = 400

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SKLKVLSY 9
           SK+KVL Y
Sbjct: 366 SKMKVLEY 373
>NP_005357
          Length = 429

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SKLKVLSY 9
           SK+KVL Y
Sbjct: 395 SKMKVLEY 402
>NP_001258681
          Length = 275

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SKLKVLSY 9
           SK+KVL Y
Sbjct: 213 SKMKVLEY 220
>NP_005859
          Length = 118

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  MSKLKVLS 8
          M KLK+LS
Sbjct: 82 MGKLKILS 89
>NP_056054
          Length = 1186

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1 MSKLKVL 7
         MSKLKV+
Sbjct: 1 MSKLKVI 7
>NP_057646
          Length = 1049

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   MSKLKVLS 8
           ++KLKVLS
Sbjct: 203 LTKLKVLS 210
>XP_005251905
          Length = 1226

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1 MSKLKVL 7
         MSKLKV+
Sbjct: 1 MSKLKVI 7
>XP_005251904
          Length = 1226

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1 MSKLKVL 7
         MSKLKV+
Sbjct: 1 MSKLKVI 7
>NP_071745
          Length = 960

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KLKVLSY 9
           +LKVLSY
Sbjct: 136 ELKVLSY 142
>NP_001123612
          Length = 960

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KLKVLSY 9
           +LKVLSY
Sbjct: 136 ELKVLSY 142
>NP_573403
          Length = 720

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SKLKVLSY 9
           SKL+ LSY
Sbjct: 497 SKLRSLSY 504
>NP_036604
          Length = 466

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  MSKLKVLSY 9
          +SKLK L Y
Sbjct: 62 VSKLKKLEY 70
>NP_055085
          Length = 487

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LKVLSY 9
           LKVLSY
Sbjct: 338 LKVLSY 343
>NP_001229965
          Length = 487

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LKVLSY 9
           LKVLSY
Sbjct: 338 LKVLSY 343
>NP_001119807
          Length = 487

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LKVLSY 9
           LKVLSY
Sbjct: 338 LKVLSY 343
>NP_877496
          Length = 720

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SKLKVLSY 9
           SKL+ LSY
Sbjct: 497 SKLRSLSY 504
>XP_005260559
          Length = 537

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SKLKVLSY 9
           SKL+ LSY
Sbjct: 314 SKLRSLSY 321
>XP_005260558
          Length = 537

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SKLKVLSY 9
           SKL+ LSY
Sbjct: 314 SKLRSLSY 321
>XP_006723918
          Length = 632

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SKLKVLSY 9
           SKL+ LSY
Sbjct: 409 SKLRSLSY 416
>XP_005260557
          Length = 632

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SKLKVLSY 9
           SKL+ LSY
Sbjct: 409 SKLRSLSY 416
>XP_005260556
          Length = 632

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SKLKVLSY 9
           SKL+ LSY
Sbjct: 409 SKLRSLSY 416
>XP_005260555
          Length = 632

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SKLKVLSY 9
           SKL+ LSY
Sbjct: 409 SKLRSLSY 416
>XP_005260554
          Length = 632

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SKLKVLSY 9
           SKL+ LSY
Sbjct: 409 SKLRSLSY 416
>XP_006723917
          Length = 720

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SKLKVLSY 9
           SKL+ LSY
Sbjct: 497 SKLRSLSY 504
>XP_005260553
          Length = 720

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SKLKVLSY 9
           SKL+ LSY
Sbjct: 497 SKLRSLSY 504
>XP_006723055
          Length = 260

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LKVLSY 9
           LKVLSY
Sbjct: 111 LKVLSY 116
>XP_005258525
          Length = 278

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LKVLSY 9
           LKVLSY
Sbjct: 129 LKVLSY 134
>XP_006716601
          Length = 469

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  MSKLKVLSY 9
          +SKLK L Y
Sbjct: 65 VSKLKKLEY 73
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.319    0.127    0.308 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 339,030
Number of extensions: 540
Number of successful extensions: 38
Number of sequences better than 10000.0: 38
Number of HSP's gapped: 38
Number of HSP's successfully gapped: 38
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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