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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004963                                                              18    1482
XP_005251512                                                           18    1482
NP_001243000                                                           16    7355
XP_005257603                                                           16    7355
XP_006722058                                                           16    7355
XP_005257602                                                           16    7355
NP_001073351                                                           15    9605
NP_001073350                                                           15    9605
NP_001073349                                                           15    9605
NP_057541                                                              15    9605
XP_005258773                                                           15    9605

>NP_004963
          Length = 1132

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  MSALMSETE 9
          M ALMSETE
Sbjct: 84 MFALMSETE 92



>XP_005251512
          Length = 1132

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  MSALMSETE 9
          M ALMSETE
Sbjct: 84 MFALMSETE 92



>NP_001243000
          Length = 5207

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    MSALMSETE 9
            +SAL S+TE
Sbjct: 2143 LSALRSDTE 2151



>XP_005257603
          Length = 5256

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    MSALMSETE 9
            +SAL S+TE
Sbjct: 2192 LSALRSDTE 2200



>XP_006722058
          Length = 5256

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    MSALMSETE 9
            +SAL S+TE
Sbjct: 2192 LSALRSDTE 2200



>XP_005257602
          Length = 5256

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    MSALMSETE 9
            +SAL S+TE
Sbjct: 2192 LSALRSDTE 2200



>NP_001073351
          Length = 721

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SALMSETE 9
           SALM E+E
Sbjct: 181 SALMDESE 188



>NP_001073350
          Length = 878

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SALMSETE 9
           SALM E+E
Sbjct: 338 SALMDESE 345



>NP_001073349
          Length = 878

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SALMSETE 9
           SALM E+E
Sbjct: 338 SALMDESE 345



>NP_057541
          Length = 878

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SALMSETE 9
           SALM E+E
Sbjct: 338 SALMDESE 345



>XP_005258773
          Length = 721

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SALMSETE 9
           SALM E+E
Sbjct: 181 SALMDESE 188



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.293   0.0995    0.211 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 243,435
Number of extensions: 187
Number of successful extensions: 11
Number of sequences better than 10000.0: 11
Number of HSP's gapped: 11
Number of HSP's successfully gapped: 11
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)