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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001275701                                                           19     665
NP_004561                                                              19     665
NP_001275703                                                           19     665
XP_005253450                                                           19     665
XP_006719151                                                           19     665
NP_000089                                                              15    9605
NP_000121                                                              15    9605
NP_055365                                                              15    9605
NP_444283                                                              15    9605
NP_003584                                                              15    9605
XP_005258103                                                           15    9605
XP_005270541                                                           15    9605

>NP_001275701
          Length = 1486

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MSAINPAKS 9
            MSAI+PAKS
Sbjct: 1220 MSAISPAKS 1228



>NP_004561
          Length = 1445

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MSAINPAKS 9
            MSAI+PAKS
Sbjct: 1179 MSAISPAKS 1187



>NP_001275703
          Length = 1264

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MSAINPAKS 9
            MSAI+PAKS
Sbjct: 998  MSAISPAKS 1006



>XP_005253450
          Length = 1486

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MSAINPAKS 9
            MSAI+PAKS
Sbjct: 1220 MSAISPAKS 1228



>XP_006719151
          Length = 1486

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MSAINPAKS 9
            MSAI+PAKS
Sbjct: 1220 MSAISPAKS 1228



>NP_000089
          Length = 658

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   INPAKS 9
           +NPAKS
Sbjct: 178 LNPAKS 183



>NP_000121
          Length = 2224

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    SAINPAK 8
            SA+NP K
Sbjct: 1732 SAVNPEK 1738



>NP_055365
          Length = 1032

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  AINPAK 8
          A+NPAK
Sbjct: 41 ALNPAK 46



>NP_444283
          Length = 240

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   INPAKS 9
           +NPAKS
Sbjct: 233 LNPAKS 238



>NP_003584
          Length = 648

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   AINPAK 8
           A+NPAK
Sbjct: 347 ALNPAK 352



>XP_005258103
          Length = 648

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   AINPAK 8
           A+NPAK
Sbjct: 347 ALNPAK 352



>XP_005270541
          Length = 635

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   INPAKS 9
           +NPAKS
Sbjct: 178 LNPAKS 183



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.300    0.107    0.270 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 581,172
Number of extensions: 275
Number of successful extensions: 12
Number of sequences better than 10000.0: 12
Number of HSP's gapped: 12
Number of HSP's successfully gapped: 12
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)